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    Home > Biochemistry News > Biotechnology News > Zhejiang University uses the Microwell-seq platform to build the world's first mammalian cell map.

    Zhejiang University uses the Microwell-seq platform to build the world's first mammalian cell map.

    • Last Update: 2020-08-11
    • Source: Internet
    • Author: User
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    Professor Guo Guoxuan of the Center for Stem Cell and Regenerative Medicine at Zhejiang University School of Medicine developed microwell-seq, a low-cost, high-efficiency, and fully domesticized high-throughput single-cell sequencing platform, and used it in a short period of time to map the world's first mammalian cell map.
    the results were published in the international academic journal Cell on the 23rd.
    cells are the smallest independent genetic units of life.
    traditional sequencing techniques "look" at a group of cells, "read" is the mean of a group of cell genetic signals, so the specific performance of individual cells is easy to ignore. According to Guo Guoxuan,
    , single-cell histology technology enables humans to accurately analyze cell differentiation, regeneration, aging and lesions from the perspective of a single cell, "and this kind of technology is leading to a methodological revolution in cell detection, classification and identification".
    "We used microporous matrix, molecular marker and amplification technology to achieve single-cell horizontal analysis with high throughput and high precision, and solved the problem of small, easy to lose and high analytical cost of a single cell in traditional sequencing.
    ," Guo said.
    researchers showed reporters a three-centimeter-long square sheet of a 100,000-diameter 30-micron-diameter "small pit" of agar sugar micropores.
    experiment, scientists used digestive enzymes to dissodivide a relatively tight cell into a single cell's suspension, dumped on a garsosugar microporous plate, about 10,000 cells will "one radish a pit" into the "pit." After
    , the researchers labeled each of the captured cells a "magnetic bead index" and tens of thousands of beads 25 microns in diameter were dumped into a "pit" and the DNA index was labeled at the same time as sealing a single cell. The third step of the
    is a routine sequencing process in which the expression spectrum of each cell is parsed.
    technology can test all messenger RNA in a single cell, an important substance that translates genetic information from cell DNA into proteins.
    using this high-throughput single-cell sequencing platform, the researchers systematically analyzed more than 400,000 cells in nearly 50 organ tissues in different stages of life in mice to create the first mammalian cell map.
    experts believe that the completion of mouse cell mapping will be the next step in the construction of human cell map guidance, and benefit from cell biology, developmental biology, neurobiology, hematology and regenerative medicine and other fields.
    Zhejiang University used the Microwell-seq platform to build a map of mouse cells Without two identical leaves and two identical cells.
    scientific advances make it possible for us to "read" every cell in the life. On February 23,
    , cell, an internationally renowned academic journal, published the findings of Professor Guo Guoxuan of the Center for Stem Cell and Regenerative Medicine at Zhejiang University School of Medicine: using microplate sequencing technology to map mouse cells.
    the team developed the low-cost, high-efficiency, fully domestically-made high-throughput single-cell sequencing platform Microwell-seq, and used it in a short period of time to build the cell map of the world's first mammalian.
    from looking at a group of cells to seeing a single cell cell is the smallest independent genetic unit of life.
    the life of the organism begins to divide from a fertilized egg and gradually develops into an individual, with the differences between cells growing and taking on different functions.
    there are differences in cells with the same function.
    extreme example is that tumor cells are originally formed by a mutation in a normal cell.
    traditional sequencing techniques "look" at a group of swarms of cells, "read" to the mean of a group of cell genetic signals, so the specific performance of individual cells is easily ignored.
    and single-cell sequencing, by "reading" the genetic information of a single cell, to deal well with the problem of cell population heterogeneity.
    that is why single-cell sequencing technology has become a hot topic for scientists in various countries, and has further promoted human understanding of the mysteries of life. according to
    Guo Guoxuan, single-cell histological technology, which enables humans to more accurately analyze cell differentiation, regeneration, aging and lesions from the perspective of a single cell, "is bringing about a methodological revolution in cell detection, classification and identification".
    October 2017, U.S. scientists, in conjunction with multinational scientists, launched the Human Cell Atlas (HCA), which envisions the classification and sequencing of all cells in the human body, the systematic mapping of human cells, and the key to deepen understanding of disease diagnosis, monitoring and treatment.
    Facebook founder Mark Zuckerberg has funded the project and called it an international research project comparable to the Human Genome Project.
    in the past few years, single-cell sequencing has shown great promise in detecting tumor mutations, prenatal diagnosis, and so on.
    how to detect individual cells quickly and efficiently in the face of the ambitious ambitions of the cell mapping program, science and technology has developed rapidly in recent years, scientists from all over the world are scrambling to develop a high-level single-cell sequencing platform: one of the key questions is: how to quickly and efficiently detect individual cells in order to finish this huge amount of super-"jigsaw puzzles"? Professor Guo guoxuan's team introduced his approach to the world on CELL: a high-volume, low-cost, simple-to-operate, fully domestic microwell-seq single-cell sequencing platform. 'We used microporous matrix, molecular marker and amplification technology to achieve single-cell horizontal analysis with high throughput and high precision, and solved the problem that a single cell nucleic acid material in traditional sequencing is too little, easy to lose, and high in cost analysis, ' said Guo Guoxuan,
    .
    Dr. Han Xiaoping (first author) showed reporters a three-centimeter-long square sheet , a microporous plate made of agar sugar, "with 100,000 'small holes' with a diameter of 30 microns."
    ", in the study, scientists used digestive enzymes to dissodivide a relatively tight cell into a single cell's suspension and then dumpititited it onto a plate of agar sugar micropores, "about 10,000 cells would fall into the pit 'one radish pit'."
    " this is the first step: capturing individual cells.
    , scientists will put a "number" on each of the cells captured, called a bead index.
    tens of thousands of beads 25 microns in diameter fall into the pit, sealing individual cells and marking them with DNA indexes.
    third step is a routine sequencing process in which the expression spectrum of each cell is parsed.
    in the technology of Professor Guo Guoxuan's team, the team measured mRNAs owned by individual cells, a genetic material that translates proteins.
    initially in preparation for the index beads, the team looked for all of China's biotechnology companies, not one can do, so the team decided to synthesize their own. "In the process, we found a large number of index primers incomplete, but we couldn't find a reason, " said Guo Guoxuan,
    .
    " After more than a month of investigation, it was found that the wrong DNA polymerase was used.
    switched to a hi-fi enzyme DNA polymerase, and everything worked out.
    Guo Guoxuan believes that the Microwell-seq technology platform is simple to operate and inexpensive, and will certainly promote the popularization and application of cutting-edge single-cell sequencing technology in basic scientific research and clinical diagnosis.
    ": "It used to take about 100 yuan to analyze a cell, but now we're developing a platform that costs less than 2 yuan.
    and experiment operation is faster and easier.
    ," Guo said.
    Guo Guoxuan's team constructed the first mammalian cell map using Microwell-seq, the team conducted systematic single-cell transcription already on more than 400,000 cells from nearly 50 organ tissues from different stages of life in mice, and constructed the first mammalian cell map.
    Guo Guoxuan called the map a "cell map".
    in the study, the team described in detail the main cell types in mice, tissue cell subtypes, matrix cell subtypes, vascular endothelial cell subtypes, and immune cell subtypes in each organ.
    click on the website now and you can see this cell map.
    website not only has an interactive data display and gene search interface, but also provides a single-cell data comparison system.
    any single-cell expression spectrum data can be analyzed through single-cell comparison to find the cell type and source of it.
    study found that matrix cells from different tissues have completely different gene expression characteristics and play an important regulatory role in tissue-specific microenvironments.
    this suggests that scientists must not only consider tissue cells in tissue engineering and organ repair, but also repair the corresponding matrix cell system.
    "Humans' perception of life is like the exploration of the vast universe, single-cell sequencing, giving us a more special 'observation' tool to explore important substances that have not been discovered in the 'starry sky' of life."
    ," Guo said.
    " scientists could use this platform to conduct institutional studies of cell fate decisions, and the identification of pre-transplant cell identification in regenerative medicine, as well as cell-level diagnosis of clinical diseases, could have far-reaching implications.
    " in the course of review, cell three review experts gave a consistent evaluation of the work "impressive", and that "mouse map will become a widely used biological data resource."
    the scientific community believes that the completion of mouse cell mapping will also be the next step in the construction of human cell map guidance, and benefit from cell biology, developmental biology, neurobiology, hematology and regenerative medicine and other fields.
    ", "Our team has actually completed a huge project on our own, and We hope that we have confidence in our life sciences industry, technology and scientists."
    Chinese is fully capable and technical in mapping a Chinese cell map of its own. "This single-cell sequencing technology is inexpensive, and all reagents and materials are fully domestic, and the commercialization prospects are very broad, " said Guo Guoxuan,
    .
    our team will continue to work hard to develop better and more grounded gas technology to contribute to the development of China's biotechnology industry.
    "Source:Zhejiang News.
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