Zushi Majiasu

Zushi Majiasu

Daphnetin

[Synonym] daphnetin , 7,8-Dihydroxycoumarin

【Structural formula】

【Molecular formula and molecular weight】C ₉ H ₆ O ₄ ;178.

[Source] The dried stem bark and root bark of Daphne giraldii Nitsche, a plant of Daphne giraldii Nitsche

【Properties】Slightly yellow fine needle-like crystals

This product is easily soluble in methanol and water

Melting point: 265～267℃

【Purity Check】

TLC

1.

Developing solvent chloroform - acetone - carboxylic acid (7:1: 0.

Check under UV light (254nm, 365nm);

After 1% FeCl ₃ ethanol solution develops color, inspect under visible light

2.

Developing agent toluene -ethyl formate - formic acid (5:4:1)

Check under UV light (254nm, 365nm);

After 1% FeCl ₃ ethanol solution develops color, inspect under visible light

High performance liquid chromatography

Column HypersilC ₁₈ , 5um (4.

Mobile phase acetonitrile-0.

Detection wavelength 330nm

【Structural identification】206,263,324

3508, 3116, 1680 (C=O), 1581, 1506, 1414, 1302, 1169

EI-MS m/z: 178[M] ⁺ , 150,143,132,122

¹ H-NMR (DMSO) δ: 10.
10 (1H, s, 7-OH), 9.
34 (1H, s, 8-OH), 7.
88 (1H, d, J=9.
6 Hz, H-4), 7.
00 (1H , D, J=8.
4 Hz, H-5), 6.
78 (1H, d, J=8.
4 Hz, H-6), 6.
16 (1H, d, J=9.
6 Hz, H-3)
.

¹³ C-NMR (DMSO) δ: 160.
4(C-2), 111.
2(C-3), 143.
7(C-4), 118.
8(C-5), 112.
5(C-6), 149.
7(C-7) , 132.
1(C-8), 145.
1(C-9), 112.
0(C-10)
.

【Storage】Dry and airtight
.