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Measurement of Plant Hormones by Liquid ChromatographyMass Spectrometry
Time of Update: 2021-01-18
Accurate and sensitive quantification of ABA and its metabolites has made a significant contribution to the knowledge of the role of this hormone, and also of its relationship to the induction of numerous ABA-induced genes in plants.
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Transformation of Cereals by Microprojectile Bombardment of Immature Inflorescence and Scutellum Tissues
Time of Update: 2021-01-18
It was not until 1988 and 1989 that the first transgenic cereal plants (maize [ 2 ]; rice [ 3 ]) were produced by the use of directDNA -transfer into protoplasts.
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Plastid Transformation as an Expression Tool for Plant-Derived Biopharmaceuticals
Time of Update: 2021-01-18
Further, the plastid transformation technology is an environmentally friendly method because plastid and their genetic information are maternally inherited in many species with a consequent lack of transmission of plastidDNA by pollen.
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Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System
Time of Update: 2021-01-18
We report here the use of transient RNAi to isolate regulatory factor genes involved in isoquinoline alkaloid biosynthesis inCoptis japonica protoplasts.
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Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes
Time of Update: 2021-01-18
Here we describe a method for engineering zinc-finger nucleases (ZFNs) for the purpose of creating targeted mutations in the paleopolyploid soybean genome.
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Elementary Flux Modes, Flux Balance Analysis, and Their Application to Plant Metabolism
Time of Update: 2021-01-18
Here, we describe two approaches for identification of all feasible routes through the network (elementary mode analysis) and for simulation of flux distribution in the network based on plant physiological uptake and excretion rates (flux balance analysis).
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Formulation and Application of Plant Pathogens for Biological Weed Control
Time of Update: 2021-01-18
The public’s demand for safe, biologically based weed controls has created an impetus to commercialize biological weed-control agents.
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Characterization of Rice Genes Using a Heterologous Full-Length cDNA Expression System
Time of Update: 2021-01-18
This system was applied to high-throughput analysis of rice genes through heterologous expression inArabidopsis (rice FOXArabidopsis lines).
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Isolation and Analysis of Small RNAs from Virus-Infected Plants
Time of Update: 2021-01-18
In this chapter, we detail some of the methods available to the researcher for isolating and analyzing virus-derived small RNAs (vsRNAs).
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Formulations of Biopesticides
Time of Update: 2021-01-18
ability of field performance, costs of production, and a number of technologi cal challenges, including fermentation, formulation, and delivery systems ( 1 – 42 ). Selection of the appropriate formulations that can improve product
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Induction of Embryogenesis in Callus Culture
Time of Update: 2021-01-18
It seems that the influence of a hormone, such as auxin, rejuvenates the cell so much that it reacquires the capacity of a zygote to produce an embryo.
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Organogenesis in Callus Culture
Time of Update: 2021-01-18
Research during the last 30 yr has demonstrated that successful organogenesis in callus cultures can be achieved by the correct choice of medium components, selection of a suitable inoculum, and control of the physical environment ( 1 ).
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In Vivo NMR for 13C metabolic Flux Analysis
Time of Update: 2021-01-18
The kinetic data can be used as input for dynamic (nonstationary) Metabolic Flux Analysis.
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Mutation and Mutation Screening
Time of Update: 2021-01-18
The techniques of mutation induction, dose optimization, and TILLING are explained.
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Methods for Rice Phenomics Studies
Time of Update: 2021-01-18
Thus, data on mutant lines, phenotypes, and segregation rate for all kinds of mutant populations, as well as integration sites for insertional mutant populations, would be searchable, and the collection would be a good resource for rice functional genomics study.
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Engineering High Yields of Secondary Metabolites in Rubia Cell Cultures Through Transformation with Rol Genes
Time of Update: 2021-01-18
cells with therol genes as an example of metabolic engineering of secondary metabolites.
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Biological Chemistry of Virus-Encoded Suppressors of RNA Silencing: An Overview
Time of Update: 2021-01-18
As an efficient counter-defensive strategy, most plant viruses evolved the ability to encode specific proteins capable of interfering with RNAi, and this process is commonly known as RNA silencing suppression.
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High-Frequency and Efficient Agrobacterium-Mediated Transformation ofArabidopsis thaliana Ecotypes C24 and Landsberg erecta Using Agrobacterium tumefaciens
Time of Update: 2021-01-18
Many reports have indicated the high regeneration potential of cotyledon explants at various stages of development in maturing embryos or after seed germination ( 14 – 17 ).
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Micropropagation of Helleborus through Axillary Budding
Time of Update: 2021-01-18
The described protocol provides information which can contribute to the commercial production ofHelleborus plants.
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Manipulation of Shoot Formation in Cultured Explants
Time of Update: 2021-01-18
Subsequently, their observations have been applied, extended, and found general application with numerous genera and species.
