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Dissecting mitosis by RNAi inDrosophila tissue culture cells
Time of Update: 2021-03-06
In this paper we focused particularly on the procedures for the proper phenotypic analysis of cells after RNAi-mediated depletion of proteins required for mitosis, the process by which the genetic information is segregated equally between daughter cells.
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Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells
Time of Update: 2021-03-05
In this chapter, I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential.
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Pyrophosphate-Linked Oligosaccharides Pathway in Cell Cultures and Animal Tissues
Time of Update: 2021-03-02
It is feasible to analyze almost any component in the LLO pathway with the application FACE, from sugar precursors to mature LLO (Glc 3 Man 9 GlcNAc 2 -P-P-dolichol).
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Phosphopeptide mapping of proteins ectopically expressed in tissue culture cell lines
Time of Update: 2021-02-27
We generated phosphopeptide maps of the phosphorylated HAND1 using the methods described below and linked these modifications to changes in HAND1 biological function.
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, Reliable High-Throughput Indices of Reactive Nitrogen Species in Cell-Culture Systems
Time of Update: 2021-02-24
Reactive nitrogen species (RNS) are generated as part of inflammatory reactions, mostly as a consequence of inducible nitric oxide synthase (iNOS) (reviewed in refs. 1 – 3 ).
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The study found that common glue components can be used to culture hematopoietic stem cells
Time of Update: 2021-02-22
, to help treat leukemia and other diseases. hematopoietic stem cells can differentiate into white and red blood cells in the blood and can be used to treat diseases such as leukemia. However, in the past, attempts to cultivate hematopoietic stem
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Insect Cell- Culture Techniques in Serum- Containing Medium
Time of Update: 2021-02-22
The establishment of new lines is now routine, and the culture of hundreds of cell lines from a variety of insect species have to date been reported ( 2 ,4 ,5 ,6 ).
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Expression of Tissue Inhibitor of Metalloproteinases by Recombinant Baculovirus-Infected Insect Cells Cultured in an Airlift Fermentor
Time of Update: 2021-02-22
In this system, the gene to be expressed is cloned into a plasmid transfer vector, downstream of a strong baculovirus gene promoter, and cotransfected with wildtype virusDNA into insect cells.
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A Method for Short-Term Culture of Human Gastric Epithelial Cells to Study the Effects of Helicobacter pylori
Time of Update: 2021-02-22
Here we describe a method for isolation and short-term culture of human primary gastric epithelial cells obtained from gastric biopsy specimens, and the use of these cells to evaluate the effect ofH.
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Detection of Mycoplasmas in Cell Culture by Fluorescence Methods
Time of Update: 2021-02-19
The fluorescence stains used most often for mycoplasma detection are DNA binding fluorochromes (DNAFs) and fluoresceinated antibodies.
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Rotavirus Entry into Tissue Culture Cells
Time of Update: 2021-02-16
Proteolytic cleavage of VP4 into two noncovalently associated subunits, VP8* and VP5* ( 2 , 9 , 10 ), significantly enhances viral infectivity ( 11 – 13 ).
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Short-Term Culture of CD8 Cells and Intracellular Cytokine Staining
Time of Update: 2021-02-15
In this chapter, we provide protocols for the generation of polarized populations of CD8 T effector cells using polyclonal stimulation and for their subsequent analysis by intracellular cytokine staining.
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Cell Rep: Successfully cultured conjunctival tissue in a laboratory environment
Time of Update: 2021-02-15
a new study, researchers at Osaka University produced functional conjunctivular tissue in-body by identifying key protein factors that drive the development and maturation of conjunctivular cells.
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Detection of Mycoplasmas in Cell Cultures by Cultural Methods
Time of Update: 2021-02-12
However,Mycoplasma hyorhinis DBS 1050, a strain that commonly infects cell cultures, is not readily grown on the commonly used agar preparations ( 1 ).
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Integrated Cell Culture/PCR for Detection of Enteric Viruses in Environmental Samples
Time of Update: 2021-01-31
that control measures may be developed with true virus occurrence data. The ICC/PCR approach combines two previously applied virus detection methods, conventional cell culture and PCR amplification, utilizing the major advantages and overcoming the major limitations of each methodology when used alone.
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Basic techniques for sterile operation in microbial and cell culture
Time of Update: 2021-01-21
/> technology, etc. aseptic room, microbial laboratory dedicated to a small room, outdoor set up a buffer room, buffer room door and sterile room door do not face the same direction, so as not to air flow into the clutter. Aseptic rooms
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Selection of Media for Tissue and Cell Culture
Time of Update: 2021-01-19
This chapter deals with the selection of growth media for the induction of callus and initiation of liquid suspension cultures from plants.
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Engineering High Yields of Secondary Metabolites in Rubia Cell Cultures Through Transformation with Rol Genes
Time of Update: 2021-01-18
cells with therol genes as an example of metabolic engineering of secondary metabolites.
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Large-Scale Culture of Plant Cells
Time of Update: 2021-01-16
It was soon found, however, that although plant cell suspensions appear to be similar in many ways to microbial cultures, there are, in fact, key differences that can have a significant influence on large-scale cultivation.
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Establishment and Maintenance of Cell Suspension Cultures
Time of Update: 2021-01-15
for quantitative or qualitative analysis of growth responses and metabolism of novel chemicals, as well as for studies of cell cycle under standard conditions ( 2 , 3 ). In addition, cell suspensions serve as an ideal material
