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Science Sub-Journal: Using neurons from iPS cells can be used to test treatments for Christiansen syndrome
Time of Update: 2021-02-27
study was published in the February 10, 2021 issue of the Journal of Science Translational Medicine under the title "Human neurons from Christianson syndrome ipsCs reveal mutation-specific responses to rescue strategies." images from Science Translational Medicine, 2021, doi:10.1126/scitranslmed.aaw0682.
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Quantitative Analysis of Mucins in Mucosal Secretions Using Indirect Enzyme-Linked Immunosorbent Assay
Time of Update: 2021-02-27
These protocols allow a rapid, reproducible, and sensitive assay for these large molecules.
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Diagnosis of Krabbe Disease by Use of a Natural Substrate
Time of Update: 2021-02-27
To obviate this a modified assay is used where only the radiolabeled substrate is included in the incubation.
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Analysis of Receptor Tyrosine Kinase Internalization Using Flow Cytometry
Time of Update: 2021-02-27
To understand how cMet signaling for normal cell growth is controlled by endocytosis and how these mechanisms are dysregulated in metastatic cancers, we developed flow cytometry-based assays to examine cMet internalization.
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Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes
Time of Update: 2021-02-27
Inspired by a natural fusion cytochrome P450 (P450) containing a monooxygenase domain and a reductasedomain, we have developed a heterotrimeric protein-utilized method to form a multienzyme complex composed of a bacterial P450and its catalytically essential two redox proteins.
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Cell: Tumor immunotherapy can be used to treat malignant brain cancer
Time of Update: 2021-02-27
February 15, 2021 /--- -- Scientists have identified a new target for a potentially malignant brain tumor immunotherapy, according to a recent paper published in the journal Cell.
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CD19 CAR-T therapy! Gilead Yescarta's use of corticosteroids before infusion can reduce cytokine release syndrome and neurological events!
Time of Update: 2021-02-27
from the CD19 CAR-T cell therapy Yescarta (axicabtagene ciloleucel) trial for recurring or resusciable large B-cell lymphoma (LBLC) adult patients were presented at the Conference on Transplantation and Cell Therapy. In the ZUMA-1 trial of a new
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Expression and Purification of Recombinant Proteins Using the pET System
Time of Update: 2021-02-26
Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals; expression is induced by providing a source of T7 RNA polymerase in the host cell ( 1 – 3 ).
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One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and Identification of 3H-Labeled RNA by Fluorography
Time of Update: 2021-02-26
Possible applications include examining products of in vitro synthesis, hybrid-selected RNAs from total cellular nucleic acids, and Northern blots.
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Exploring Membrane Domains Using Native Membrane Sheets and Transmission Electron Microscopy
Time of Update: 2021-02-26
Membrane sheets on EM grids are fixed, labeled with functionalized nanoprobes, and imaged by transmission electron microscopy.
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The EU has expanded the label for the use of GSK/Innoviva's slow-stop pulmonary inhalers
Time of Update: 2021-02-26
The European Commission has expanded glaxoSmithKline/Innoviva's once-a-day Trelegy Ellipta label to make it the first single-inhaled triple therapy for patients with moderate to severe slow-blocking lungs (LCPs), who are treated with corticosteroids
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NEJM: Avacopan is used to treat ANCA-related vasculitis
Time of Update: 2021-02-26
the researchers conducted a Phase 3 randomized trial (ADVOCATE) that compared the role of avacopan and the d diminishing number of Ponieson in patients with ANCA-related vasculitis who were treated with immunosuppressive drugs at the same time.
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Abcam Finance is used to expand the research bio-reagent sales platform
Time of Update: 2021-02-26
HSBC has also provided abcam with revolving credit to help it develop new technologies and expand its market.
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San Francisco followed Germany's example of using waterproof paint to deal with uncivilized
Time of Update: 2021-02-26
It that this method has recently been used by the Municipality of Hamburg, Germany.
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The Preparation of Phenyl-stilbene Derivatives Using the Safety Catch Linker
Time of Update: 2021-02-26
One ion channel that controls lymphocyte differentiation is the highly T cell specific Kv1.3 channel.
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New Crown Vaccine Express! WHO adds AstraZeneta AZD1222 to its list of emergency uses: routine
Time of Update: 2021-02-26
mentioning that the COVID-19 Vaccine AstraZeneca vaccine can be stored, transported and treated for at least 6 months under conventional refrigeration conditions (2-8 degrees C/36-46 degrees Fahrenheit) and used in existing medical settings.
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Preparation of Oriented, Fully Hydrated Lipid Samples for Structure Determination Using X-Ray Scattering
Time of Update: 2021-02-26
This chapter describes a method of sample preparation called “the rock and roll method,” which is basically a solvent evaporation technique with controlled manual sample movement during evaporation of solvent from lipid/solvent mixtures
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Chiral Separation by Capillary Electrophoresis Using Polysaccharides
Time of Update: 2021-02-26
Capillary electrophoresis (CE) offers numerous advantages, such as high separation efficiency, analysis speed, instrumentation simplicity, and reduced operating costs, compared with other separation analytical methods.
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BMJ: Nearly 3 million population data reveal the relationship between opioid use during pregnancy and congenital malformations in offspring
Time of Update: 2021-02-26
, the study showed that the use of prescription opioids in early pregnancy was not associated with the risk of most considered types of malformation, but led to an increased risk of cleft palate and cleft mouth in future generations.
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An effective method of completely removing contaminating genomic DNA from an RNA sample to be used for PCR
Time of Update: 2021-02-25
This method produces an RNA sample that is negative for genomic DNA byPCR .
