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    biotinylated dna probe

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    • Detection of DNA Sequences Using Biotinylated Probes

      Time of Update: 2021-02-28
      It was found (1 ) that biotinylated analogs of dUTP, which contain a biotin molecule covalently bound to the C-5 position of the pyrimidine via an allylamine linker arm, can be used as substrates forDNA polymerase. Figure 1 shows the structure of biotin-11-dUTP, a commonly used substrate for biotinylation by the method of nick translation (2 ).

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