full-length transcript annotation of Fusarium graminearum, revealing the regulatory functions of alternative splicing and variable polyadenylation
Time of Update: 2022-05-17
This paper uses a variety of technologies such as full-length transcriptome Iso-Seq, strand-specific RNA-Seq and gene function verification to create a comprehensive and complete set of filamentous plant pathogenic fungi, Fusarium graminearum.
Single-cell structural and functional requirements for alternative splicing during hematopoietic stem cell formation
Time of Update: 2022-01-26
Here, we used single-cell full-length transcriptomic data to construct a subtype-based transcriptional profile of mouse endothelial cell-to-hematopoietic stem cell (HSC) transformation that was able to identify blood-derived signature subtypes and stage-specific AS events .
mediated by long non-coding RNA alternative splicing isoforms
Time of Update: 2021-08-16
On the basis of the preliminary work of the research group, on June 10, 2021, Wang Xiangting's research group published a titled Long non-coding RNA PM maintains cerebellar synaptic integrity and Cbln1 activation via Pax6/Mll1-mediated H3K4me3 on PLOS Biology and published it in the journal The official website is highly recommended .
Imaging Alternative Splicing in Living Cells
Time of Update: 2020-12-26
This method has broad applications, including the study of other alternativelyspliced genes in tissue culture and in whole animals, and may be useful in creating imaging markers for the study of tumorprogression and metastasis.
In this chapter, the authors present one example of this method using fluorescence reporters.
Using Single-Strand Conformational Polymorphism Gel Electrophoresis to Analyze Mutually Exclusive Alternative Splicing
Time of Update: 2020-12-12
We have expanded the utility of this technique to analyze easily the alternative splicing of pre-mRNAs containingmultiple mutually exclusive exons of the same size.
The ease and the quantitative nature of this technique should be very useful.
Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis
Time of Update: 2020-11-19
BecauseRPS4 expression levels are very low and the pattern ofRPS4 splicing is complex, existing mRNA quantification methods were not adequate.
This method is especially suitable for quantification of alternative transcripts of low-expressed genes exhibiting complex splicing patterns.