ex vivo vivo

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Protocol for Ex Vivo Incubation of Drosophila Primary Post-embryonic Haemocytes for Real-Time Analyses

 Time of Update: 2021-03-04

The current method employs an ex vivo system, in which post-embryonic haemocytes are isolated for short-term analysis, in order to investigate various aspects of their behaviour during events requiring cytoskeleton dynamics and Rho GTPase signalling.
In Vivo Detection of Transition Metals and Nitrosyl-Heme Complexes Using Ex Vivo Electron Paramagnetic Resonance Spectroscopy

 Time of Update: 2021-03-01

Under normal (nonstress) conditions, the cell maintains a strong reducing environment that favors reductive over highly compartmentalized oxidative biochemistry. Environmental stress can shift the redox balance away from reductive biochemistry however, promoting transition metal activation, and nonprogrammed oxidative and/or nitrosative reactions.
An Ex Vivo Erythrocyte Model for Investigating Oxidative Metabolism

 Time of Update: 2020-12-21

Human and animal erythrocytes can be loaded with different kinds of molecules (enzymes, metabolites, immunoglobulins, exogenous substances, etc.) to modify cellular metabolism, through procedures easy to perform, reproducible, and highly conservative ( 1 , 2 ). Use of these procedures, notably those based on hypotonic hemolysis, isotonic resealing, and reannealing leads to intact and fully viable erythrocytes.

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