TotalDNA restriction pattern analysis is one of the techniques commonly used to identify and group bacteria ( 1 – 11 ). This technique can be used to separate species belonging to the same genus (
Bacterial Identification and Subtyping Using DNA Microarray and DNA Sequencing
Time of Update: 2021-02-17
In this chapter, we will review (1) the use of DNA microarray using fluorescence and infrared imaging detection for identification of pathogenic bacteria, and (2) use of pyrosequencing in DNA cluster analysis to fingerprint bacterial phylogenetic trees.
Preparation of Bacterial Genomic DNA
Time of Update: 2021-02-16
The isolation of genomicDNA from a microorganism generally comprises three stages: cultivation of the cells, disruption to release cell contents, and chemical purification of the DNA. Two widely used methods
Fungal Genomic DNA Extraction
Time of Update: 2021-02-16
Transfer top phase to fresh 1.5mL microfuge tube, add 1/2 vol.
Preparation of Bacterial Plasmid DNA
Time of Update: 2021-02-15
The methods presented here were developed for isolating plasmids from the Gram-negative bacteriumLegionella pneumophila .
Extraction of DNA from Mycoplasmas
Time of Update: 2021-02-14
The manipulation of genetic material for the purpose of diagnosis or analysis almost always requires the preparation of sample to expose genomic nucleic acid or the extraction and purification of DNA .
A novel method of growing fungi for DNA extraction
Time of Update: 2021-01-30
Miniprep DNA (b-d), ITS 1-4 amplification (e-g) and HinfI restriction digests (h-j) from fungi grown on malt extract reverse agar.
Bacterial DNA extraction scheme
Time of Update: 2021-01-21
CTAB/NaCl method extracts DNA with higher purity, fewer protein impurities, and long shelf life, and editors prefer to add the final concentration of 20 μg/ml RnaseA to step 5, so that there is no RnaseA contamination in the end.
DNA Extraction from Vegetative Tissue for Next-Generation Sequencing
Time of Update: 2021-01-15
In this chapter we describe a simple two-reagent DNA extraction protocol which yields a high quality and quantity of DNA which can be used for different applications including NGS.
Geminivirus Isolation and DNA Extraction
Time of Update: 2021-01-13
Geminiviruses, named for their unique geminate capsid morphology, have small single-stranded (ss) circular DNA genomes that replicate in the nuclei of infected cells via a double stranded (ds) DNA intermediate. They are responsible
DNA Directed in Vitro Protein Synthesis with Escherichia coli S-30 Extracts
Time of Update: 2020-12-29
This extract contains the machinery necessary for the transcription and translation, i.e., ribosomes and RNA polymerase.
Experts at the Chinese Academy of Sciences extracted DNA information from the tomb 2,600 years ago
Time of Update: 2020-12-11
) for bone sample collection, in order to extract relevant DNA information, to explore the integration of this ethnic group and the Central Plains population, interactive behavior, for national integration to provide more reference basis. recently