-
Quick and reliable method to analyze meiotic segregation patterns in Coprinus cinereus using the polymerase chain reaction
Time of Update: 2021-02-18
The following components were added to the indicated concentrations after the first denaturation step for PCR-amplification (final volume 100 microliters): 67 mM Tris-HCl (pH 8.8), 16 mM (NH4)2SO4, 10 mM 2-mercaptoethanol, 3 mM MgCl2, 2 glycerol, 1 micromole of each primer (see below), dATP, dCTP, dGTP (each 200 uM), dUTP (500 uM), and 2.5 units of Amplitaq DNA Polymerase (Perkin Elmer Cetus).
-
Plasmon-Waveguide Resonance Spectroscopy Studies of Lateral Segregation in Solid-Supported Proteolipid Bilayers
Time of Update: 2020-12-05
Resonance excitation by a polarized continuous wave (CW) laser above the critical angle for total internal reflection generatesplasmon and waveguide modes, whose evanescent electromagnetic fields are localized on the outer surface and interact withthe immobilized sample (in the present case a proteolipid bilayer).
-
Langmuir Films to Determine Lateral Surface Pressure on Lipid Segregation
Time of Update: 2020-12-02
In the present chapter, some detailed protocols are described on how to prepare phospholipid Langmuir films, obtain structural information from their compression isotherms, and study their structure eitherin situ at the interface or on transfer onto solid supports by applying different microscopy techniques.
-
Beijing New Origin Market Centralized Segregation Personnel Will Implement Classification To Lift Segregation Measures in Batches
Time of Update: 2020-07-12
Today (6th) afternoon, Beijing held the 143rd outbreak prevention and control press conference At the meeting, The Beijing Municipal Center for Disease Prevention and Control Center, Pang Xingxing