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Measuring solution viscosity and its effect on enzyme activity
Time of Update: 2021-03-07
According to Kramers theory, solvent viscosity results in friction against proteins in solution, and this should result in decreased motion, inhibiting catalysis in motile enzymes.
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By force cdmo gradually become the pharmaceutical enterprises research and development production activities of the optimal solution
Time of Update: 2021-03-06
CDMO's hardware and software capabilities are reflected differently in meeting the core requirements of the two main types of customers of large pharmaceutical companies and innovative small and medium-sized pharmaceutical enterprises.
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Functional Analysis of Rho GTPase Activation and Inhibition in a Bead-Based Miniaturized Format
Time of Update: 2021-03-05
Here, we describe a bead-based protein–protein interaction assay for overexpressed HA-tagged Rho GTPases to study the GTPγS-dependent interaction with the regulatory protein RhoGDIα.
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Biopharmaceutical companies are active in IPOs, and another batch of pharmaceutical companies have filed applications
Time of Update: 2021-03-05
/> it is understood that a group of biopharmaceutical enterprises that have declared the IPO of the Company are mainly involved in innovative pharmaceutical research and development, innovative pharmaceutical services, original drugs, in-body diagnostic products
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Large-scale deciphering of human tissue gene activity
Time of Update: 2021-03-05
are expressed. However, there is still limited research on differences in gene regulation and gene expression between different human tissues and individuals. The Genotype-Tissue Expression (GTEx) Research Alliance collected and studied more
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Libang Tmall Home Wear Festival strong attack value discount activities colorful
Time of Update: 2021-03-03
China Paint Network: melting snow and ice, the temperature rebounded, the home improvement market also began to gradually heat up, ushered in the peak decoration. Ali Taobao recently "heavy hit", the strong launch of Tmall decoration season
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A Fluorescence-Based Assay for Core 1 3Galactosyltransferase (T-Synthase) Activity
Time of Update: 2021-03-03
Emerging evidence has shown the importance of O-glycans and the absolute requirement of T-synthase in this pathway.
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Nanning City Bureau of Industry and Commerce to carry out building materials consumption 3.15 theme publicity activities
Time of Update: 2021-03-03
on display, through the circuit press for unqualified aluminum profile products unified crushing treatment, to show the community the law enforcement authority of the industrial and commercial sector, reflecting the determination of the industrial
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Lipase Activation and Stabilization in Room-Temperature Ionic Liquids
Time of Update: 2021-03-03
Although lipase is used as a model enzyme, this approach may be effective in activating and stabilizing virtually any enzyme in RTILs.
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Methods for Measuring Class I Membrane-Bound Hyaluronan Synthase Activity
Time of Update: 2021-03-03
Three radioactive and nonradioactive assay methods are described that can give the amount of HA made with or without information about the distribution of product sizes.
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Imaging -Galactosidase Activity In Vivo Using Sequential Reporter-Enzyme Luminescence
Time of Update: 2021-03-02
In order to expand the application of bioluminescence imaging to other enzymes, we characterized a sequential reporter-enzyme luminescence (SRL) technology for the in vivo detection of β-galactosidase (β-gal) activity.
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Measurement of Nitric Oxide Synthase Activity In Vivo and In Vitro by Gas Chromatography-Mass Spectrometry
Time of Update: 2021-03-02
Thus, the GC-MS nitrite/nitrate assay provides information about biologically relevant NOS activity.
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High-Throughput Screening of Phosphodiesterase Activity in Living Cells
Time of Update: 2021-03-02
We present here a sensitive optical method for monitoring cAMP levels and PDE activity near the membrane, using cyclic nucleotide-gated (CNG) ion channels as sensors.
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Assay of Intracellular Hydrogen Peroxide Generation in Activated Cytometry Individual Neutrophils by Flow
Time of Update: 2021-03-01
It provides a sensitive assay to measure intracellular ROS in individual neutrophils ( 2 , 3 ).
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In Vitro Assays for Kinetoplastid U Insertion-Deletion Editing and Associated Activities
Time of Update: 2021-03-01
This chapter describes biochemical assays that have been used in analyzing RNA editing in kinetoplastid mitochondria and to characterize the general mechanism of editing by the editosome. Studies using these assays have shown
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Beijing: People don't wear masks for outdoor activities
Time of Update: 2021-03-01
According to the guidelines, members of the public are not allowed to wear masks when outdoor activities and when they are in close contact with people other than their peers.
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Application of a colorimetric assay to identify putative ribofuranosylaminobenzene 5-phosphate synthase genes expressed with activity inEscherichia coli
Time of Update: 2021-03-01
Given the importance of RFAP synthase in H 4 MPT biosynthesis, the identification of putative RFAP synthase genes and measurement of RFAP synthase activity would provide an indication of the presence of H4MPT in untested microorganisms.
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Erythropoietin and Engineered Innate Repair Activators
Time of Update: 2021-02-28
Recently, engineered molecules based on EPO’s structure–activity relationshipshave been developed that are devoid of hematopoietic effects.
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De Gaule held a security day activities: full participation in the continuous promotion of interdependence
Time of Update: 2021-02-28
Security Day: Full participation in the continuous promotion of interdependence Chairman Mr. Eric Bergé's review of the 2014 safety performance of the Pailey Group worldwide and acknowledged the Progress made by the Group in its security work.
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Cancer Res: The mechanism by which tumor-related macrophage activity is inhibited
Time of Update: 2021-02-27
once LXR is activated, the gene expression spectrum of tumor-related macrophages changes significantly, resulting in a decrease in their ability to produce immune-functioning molecules in the tumor micro environment," the study said.
