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coli recombination system

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Generation of Recombinant Adenovirus Using the Escherichia coli BJ5183 Recombination System

 Time of Update: 2021-01-26

The method described here uses homologous recombination machinery ofEscherichia coli BJ5183 to construct plasmids used in generation of adenoviral vectors. With this method, no ligation steps are involved in generating the plasmids, and any region of the adenoviral genome can be easily modified.

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