-
Synthesis by high-efficiency liquid-phase (HELP) method of oligonucleotides conjugated with high-molecular weight polyethylene glycols (PEGs)
Time of Update: 2021-03-06
In addition to chemical alterations of the backbone and of the heterocyclic bases, their conjugation with amphiphylic moieties, such as the polyethylene glycol has been proposed.
-
Detection of Estrogen Receptor by In Situ Hybridization
Time of Update: 2021-03-02
Furthermore, we reported the expression of estrogen receptor mRNA in bovine retina ( 4 ).
-
Measuring Ubiquitin Conjugation in Cells
Time of Update: 2021-02-26
Here, we describe in detail the steps to follow in order to determine whether a particular protein might become ubiquitinated, or deubiquitinated, and we offer warnings about pitfalls to avoid in antiubiquitin immunoblotting.
-
Improved Cellular Uptake of Antisense Peptide Nucleic Acids by Conjugation to a Cell-Penetrating Peptide and a Lipid Domain
Time of Update: 2021-02-26
Although chemical conjugation to a variety of CPPs significantly improves the cellular uptake of PNAs, the bioavailability (and hence antisense activity) of CPP–PNA �conjugates is still highly limited by endocytotic entrapment.
-
Bilirubin and Its Carbohydrate Conjugates
Time of Update: 2021-02-26
Important cyclic tetrapyrroles in nature related to heme are chlorophylls, which contain magnesium and are derived from protoporphyrin-IX, and vitamin B 12 , a corrinoid derived from uroporphyrinogen-III.
-
Effects of Estrogen Administration on the Colonization Capability of Lactobacilli and Escherichia coli in the Urinary Tracts of Mice
Time of Update: 2021-02-21
The first step in designing a probiotic product is to isolate and characterize strains with some beneficial properties.
-
Two Efficient Methods for the Conjugation of Smooth-Form Lipopolysaccharides with Probes Bearing
Time of Update: 2021-02-16
This chapter presents a conjugation method for coupling probes bearing hydrazine or primary amino groups to a smooth(S)-form lipopolysaccharide (LPS).
-
Scientists have found hidden special estrogen-like estrogen in breast epithal cells
Time of Update: 2021-02-12
In a recent study published in the international journal Nature Communications, scientists from the Federal Institute of Technology (EPFL) in Lausanne, Switzerland, studied the discovery of hidden special estrogen-like polyps in breast epithal cells
-
Br J Cancer: CYP3A7 rs45446698 SNP: Factors associated with pre-menopaan estrogen and progesterone
Time of Update: 2021-02-12
estrogen-3-glucosides and progesterol-3-glucosides are urine metabolites of estrogen and progesterone, respectively, in a study designed to identify genetic mutations associated with levels of sex hormones in the urine and the risk of breast cancer.
-
Effect of curing agent dosage on the performance of water-based epoxy coatings
Time of Update: 2021-02-06
/> Figure 6 is a port diagram of 30d soaked in 3.5% Of the NaCl aqueous solution under different curing agent dosages. Figure 6 Port diagram as can be seen from Figure 6, at different frequencies, the law of change of AC impedance value
-
Conjugation of Haptens
Time of Update: 2021-02-01
/>epitope and must be of sufficient size to initiate lymphocyte activation necessary for an antibody response. In practice, small chemical compounds (haptens) are generally not good immunogens. However, when attached to macromolecules (carriers
-
Nat Med: Androgen-like: anti-cancer factor for estrogen-positive breast cancer
Time of Update: 2021-01-30
gland pathlines. androgen therapy strategy was eventually terminated due to toxic side effects and the emergence of drugs that directly targeted ER. currently, ER inhibitors are the standard treatment for ER-plus breast cancer
-
Two Efficient Methods for the Conjugation of Smooth-Form Lipopolysaccharides with Probes Bearing
Time of Update: 2021-01-27
Minnesota with dansyl hydrazine and horseradish peroxidase yields labeling ratios above 110 nmol dansyl/mg LPS, with nearly no loss of the original endotoxic activity.
-
Quantifying Plasmid Copy Number to Investigate Plasmid Dosage Effects Associated with Directed Protein Evolution
Time of Update: 2021-01-26
We have observed that when random mutations are not restricted to the coding sequence of the target genes, directed evolution results in a strong positive selection of plasmid origin of replication ( ori ) mutations.
-
Effect of dispersant structure and dosage on the performance of latex paint (2)
Time of Update: 2021-01-21
1 shows that of the 3 dispersants selected, the S110 preparation coating has the smallest color difference and the best water resistance before and after immersion under the premise of the same dosage.
-
AstraZenecon has submitted a clinical application for a new generation of oral estrogen-influenced antagonists
Time of Update: 2021-01-15
AZD9833 belongs to a new generation of oral selective estrogen inhibitors (ER alpha) antagonists and degradation agents (SERDs).
-
Single-Step Conjugation of Antibodies to Quantum Dots for Labeling Cell Surface Receptors in Mammalian Cells
Time of Update: 2021-01-05
We describe herein methods that allow conjugation of antibodies to QDs in a single step without the formation of side products.
-
Chemical Microarrays Constructed by Selective Attachment of Hydrazide-Conjugated Substances to Epoxide Surfaces and Their Applications
Time of Update: 2021-01-04
The microarrays constructed by this immobilization method are used to evaluate protein binding to carbohydrates, peptides, and small molecules.
-
The fourth batch of national drug collection officially started! 90 dosage forms shortlisted
Time of Update: 2021-01-01
by the fourth batch of national organizations was officially opened. The following is the full text of the notice: Pharmaceutical-related enterprises: in order to further do a good job in the fourth batch of national organization of centralized
-
An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage
Time of Update: 2020-12-29
To measure rates of sumoylation and isopeptidase cleavage in vitro, we developed an enzyme assay that is based on fl uorescence resonance energy transfer (FRET).
