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Real-Time Quantitative Polymerase Chain Reaction Analysis of Mitochondrial DNA Point Mutation
Time of Update: 2021-02-24
Molecular diagnosis of the disease requires both qualitative detection of the mutation and quantitative analysis of the mutant heteroplasmy.
In this chapter, we describe the detailed procedures regarding the application of fluorescent probes, and real time quantitative PCR in the molecular diagnosis of mitochondrial DNA disorders.
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Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction
Time of Update: 2021-01-16
Thermal asymmetric interlaced polymerase chain reaction (TAIL- PCR ) is an efficient tool for the recovery ofDNA fragments adjacent to known sequences.
The amplified products are suitable as templates for direct sequencing, or for cloning in vectors.
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Direct DNA Sequencing of Polymerase Chain Reaction Products Using Magnetic Beads
Time of Update: 2020-12-08
The use of magnetic particles in many fields of biochemistry, molecular biology, and medicine has been well documented and several magnetic particles are now available for diagnostic and cell separation purposes.
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Progress has been made in the design of G-four-chain/hemin DNA structure and in the study of catalytic activity.
Time of Update: 2020-09-15
The G-four-stranded body is a DNA secondary structure with four strands of nucleotide chains connected by one or more rich G sequences through the Hoogsteen hydrogen bonds within or between molecules,
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Scientists have discovered the molecular mechanism by which bacteria argonaute proteins generate and load DNA-guided chains.
Time of Update: 2020-09-11
On March 2, the journal Molecular Cell published online the latest research by Wang Yanli of the Institute of Biophysics of the Chinese Academy of Sciences and his collaborators on the independent generation and loading of DNA-guided chains by the bacteria Argonaute (Ago) protein, entitled Autoomous Generation and Loading of DNA Guides by Bacterial Argonaute.
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TtAgo is able to independently generate and selectively load functional DNA guide chains.
Time of Update: 2020-09-11
On March 2, the journal Molecular Cell published online the latest research by Wang Yanli of the Institute of Biophysics of the Chinese Academy of Sciences and his collaborators on the independent generation and loading of DNA-guided chains by the bacteria Argonaute (Ago) protein, entitled Autoomous Generation and Loading of DNA Guides by Bacterial Argonaute.
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The RNA anti-chain exchange guided by the congener recombinant protein Rad52 repairs the damaged DNA.
Time of Update: 2020-08-31
process of recombination, which exchanges genetic information between two DNA molecules, plays a crucial role in DNA repair, but specific genetic mutations can disrupt gene stability.
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NMR structure of the V-shaped G-quad-chain complex between DNA molecules.
Time of Update: 2020-08-06
Recently, Zhang Sodium, a researcher at the Center for Strong Magnetic Field Science at hefei Material Science Institute of the Chinese Academy of Sciences, used nuclear magnetic resonance (NMR) technology to analyze the intermolecular V-shapeD G-tetra-chain composite structure formed by the telomere DNA sequence d (G2T4G4T4G4) and the short-chain d (TG4A) probe for the first time, and found that the new short-chain DNA probe can specifically identify the telomere sandaldna containing V-shape key base sequence.