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Metal IonRNA Interactions Studied via Multinuclear NMR
Time of Update: 2021-03-06
Nuclear magnetic resonance ( NMR ) spectroscopy offers several ways to study the RNA–metal ion interactions at an atomic level.
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Electrophoretic Mobility Shift Assay for Characterizing RNAProtein Interaction
Time of Update: 2021-03-04
The use of EMSA can provide both general and specific information concerning the interaction between two macromolecules such as RNA and protein.
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Laurdan Studies of Membrane Lipid-Nicotinic Acetylcholine Receptor Protein Interactions
Time of Update: 2021-03-04
The extrinsic fluorescent probe Laurdan (6-dodecanoyl-2-dimethylamino naphthalene) exhibits extreme sensitivity to the polarity and to the molecular dynamics of the dipoles in its environment. Dipolar relaxation processes are reflected
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Two-Step Cross-linking for Analysis of ProteinChromatin Interactions
Time of Update: 2021-03-01
Eukaryotic gene regulation is controlled, in part, by inducible transcription factor-binding regulatory sequences in a tissue-specific and hormone-responsive manner. The development of methods for the analysis of transcription factor
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Capture of Intact Liposomes on Biacore Sensor Chips for ProteinMembrane Interaction Studies
Time of Update: 2021-02-28
This chapter describes the protocol for immobilization of liposomes on L1 sensor chips and discusses some of the experimental considerations.
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Nonradioactive Methods for the Detection of RNA-Protein Interaction
Time of Update: 2021-02-25
Thus, the genome of human immunodeficiency virus (HIV) encodes proteins, Tat, Rev and NC, that bind to specific viral RNA motives ( 1 , 2 ).
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Kinetic Exclusion Assays to Study High-Affinity Binding Interactions in Homogeneous Solutions
Time of Update: 2021-02-24
In contrast, a number of heterogeneous methods that do exhibit high sensitivity have been described for the quantification of high-affinity antibody-binding interactions ( refs.
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Use of surface plasmon resonance for the measurement of low affinity binding interactions between HSP72 and measles virus nucleocapsid protein
Time of Update: 2021-02-24
In the present work, BIAcore instrumentation was used to monitor binding reactions between HSP72 and naturally occurring sequence variants of the measles virus (MV) nucleocapsid protein (N), a structural protein regulating transcription/replication of the viral genome.
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Affinity Capillary Electrophoresis to Examine Receptor-Ligand Interactions
Time of Update: 2021-02-24
Subsequent Scatchard analysis using noninteracting markers realizes a binding constant.
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Ultrastructural Analysis of BacteriaHost Cell Interactions
Time of Update: 2021-02-21
Electron microscopy of bacterial pathogens and interactions between bacteria and host cells and tissues provides valuable insights into structural and molecular properties and processes involved in pathogenesis. Applications for electron
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Analysis of Protein Factors that Interact with Adenovirus Early Promoters
Time of Update: 2021-02-19
On account of their relatively small, defined genomes, viruses have long been a convenient model system in which to study eucaryotic gene expression. Usually, viral infection is followed by early gene expression, which allows the subsequent
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DNA Microarray to Analyze AdenovirusHost Interactions
Time of Update: 2021-02-19
induced by vector genomic DNA, along with the activation of specific innate immune pathways that can be ascribed to viral particles. We herein describe a detailed protocol for the use of gene chips to dissect the toxicogenomic signature of human and canine
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In Vitro and In Vivo Interactions of Nonpathogenic Bacteria With Immunocompetent Cells
Time of Update: 2021-02-16
To date, the contribution of the intestinal epithelial cell in the first-line defense against pathogenic bacteria and microbial antigens has been recognized.
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Scientists map the interaction between human and viral proteins
Time of Update: 2021-02-15
This time, Columbia University researchers developed a computational framework P-HIPSTer, using protein structure information to infer the interaction between viral proteins and human proteins, can effectively compensate for the lack of scalability of high-flux methods.
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Fluorescence Fluctuation Spectroscopy and Imaging Methods for Examination of Dynamic Protein Interactions in Yeast
Time of Update: 2021-02-15
Protein interactions are inherently dynamic.
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Interaction Between Lactic Acid Bacteria and Gastrointestinal Nematodes of Caprine Origin
Time of Update: 2021-02-15
They have generally shown that goats were more infected than sheep, as they exhibited higher worm burdens and egg excretion.
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Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast
Time of Update: 2021-02-15
The accurate replication and expression of genetic information is ultimately governed by the interaction of regulatory proteins with specific sites on chromosomes.
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Mapping DNA Interaction Sites of Chromosomal Proteins: Crosslinking Studies in Yeast
Time of Update: 2021-02-14
Only sequences specifically bound by the regulatory protein in vivo coimmunoprecipitate with this factor (the shaded area in the P1/P2 region symbolizes an interaction site) and will give rise to PCR products from the PRECIPITATE(F) .
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General M13 Phage Display: M13 Phage Display in Identification and Characterization of ProteinProtein Interactions
Time of Update: 2021-02-13
In M13 phage display, proteins and peptides are exposed on one of the surface proteins of filamentous phage particles and become accessible to affinity enrichment against a bait of interest.
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BMC Gastroenterology: The effect of the interaction between obesity and high uric acidemia on the severity of non-alcoholic fatty liver disease
Time of Update: 2021-02-10
same time, the researchers found that in obese NAFLD alone, high uric acidemia was associated with a higher risk of severe liver fat degeneration (2.02 (1.14-3.57)) and elevated ALT (2.27 (1.37-3.76)), as was not the case in non-obese subjects.
