-
Li Long's group from the School of Life Sciences reveals the dynamic changes of the phospholipid transporter flippase in the cell membrane
Time of Update: 2022-04-19
This paper reports the conformational changes of phospholipid transferase (lipid flippase) in different lipid membranes, and discovers the resting state of this protein family for the first time, which provides an important molecular basis for understanding the formation of cell membrane asymmetry .
-
Li Xinjian's team revealed that the glucose transporter GLUT1 maintains its plasma membrane positioning and promotes...
Time of Update: 2021-10-22
On October 7, 2021, the Li Xinjian research team of the Institute of Biophysics, Chinese Academy of Sciences published an online publication in the journal Nature Communications entitled "DHHC9-mediated GLUT1 S-palmitoylation promotes glioblastoma glycolysis and tumorigenesis" (DOI: 10.
-
of the protein machine LolCDE, systematically revealing the recognition and transport mechanism of the outer membrane lipoprotein of Gram-negative bacteria
Time of Update: 2021-04-14
Responsible Editor | xi At present, the infection of pathogenic microorganisms or super bacteria with multi-drug resistance has brought a major threat to human health. Among them, gram-negative bact
-
Nature: Reveal the mechanism of action of glutamate transporters in cell membranes
Time of Update: 2021-03-24
Professor Renae Ryan, the co-corresponding author of the paper and the University of Sydney School of Medicine and Health, said, “Using cryo-EM, we have uncovered for the first time how glutamate transporters perform multi-tasking---performing dual functions, that is, in the cell membrane.
-
Noninvasive Microelectrode Ion Flux Estimation Technique (MIFE) for the Study of the Regulation of Root Membrane Transport by Cyclic Nucleotides
Time of Update: 2021-03-11
These changes are mediated by the activity of plasma-membrane based transport proteins many of which are controlled by cyclic nucleotides and/or other signaling molecules.
This chapter details basic protocols enabling the application of the MIFE technique to study regulation of root membrane transport in general and cyclic nucleotide mediated transport in particular.
-
Electrogenic Bromosulfalein Transport in Isolated Membrane Vesicles: Implementation in Both Animal
Time of Update: 2021-01-16
The spectrophotometric assay of electrogenic bromosulfalein transport in membrane vesicles, isolated from various mammalian organs or tissues, enables to specifically measure the transport activity of bilitranslocase (TCDB 2.A.65.1.1).
-
Measurement of the Energetics of Protein Transport Across the Chloroplast Thylakoid Membrane
Time of Update: 2021-01-01
Protein transport across cellular membranes represents an unknown, possibly significant drain on the total energy pool.
The methods derive from spectroscopic techniques, well known in the field of thylakoid energetics, to monitor the light-dependent ΔpH across the membrane and the rate of proton flux through the thylakoid lumen, combined with those to measure the rate of protein transport across the thylakoid membrane.
-
Fluorescence Assays for Measuring Fatty Acid Binding and Transport Through Membranes
Time of Update: 2020-12-03
In this chapter, detailed methods are provided on how to monitor the transport of FA through protein-free model membranes, and some of the fluorescent artifacts that may arise with the use of these probes are addressed.
-
Saturation-Recovery Electron Paramagnetic Resonance Discrimination by Oxygen Transport (DOT) Method for Characterizing Membrane Domains
Time of Update: 2020-12-01
The discrimination by oxygen transport (DOT) method is a dual-probe saturation-recovery electron paramagnetic resonance approachin which the observable parameter is the spin-lattice relaxation time ( T 1 ) of lipid spin labels, and the measured value is the bimolecular collision rate between molecular oxygen and the nitroxidemoiety of spin labels.
-
Tracking the Transport of E-Cadherin to and From the Plasma Membrane
Time of Update: 2020-11-24
The loss of E-cadherin from the plasma membrane is an early indication of EMT and a marker of poor prognosis in many cancers making the trafficking of E-cadherin an area of great interest.
Additionally, immunofluorescence data demonstrated that cells lacking PIPKIγ lost E-cadherin at the plasma membrane.
-
Genome-Wide Analysis of Membrane Transport Using Yeast Knockout Arrays
Time of Update: 2020-11-24
The transport of membrane-bound proteins through post-Golgi compartments depends on the coordinated function of multiple genes that direct the recognition and routing of protein cargoes to their final cellular destination.
The potential of this approach is limited only by the availability of transport assays suitable for the high-throughput screening of yeast colony arrays.
-
Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays
Time of Update: 2020-11-18
In addition to the isolation of thylakoids, this chapter also describes the experimental steps of both protein translocation across the thylakoid membrane and protein integration into the membrane.
Protein translocation and integration can be analysed by the radioactive labelling of substrate proteins using an in vitro transcription and translation system.
-
Transcriptome Analysis of Membrane Transporters in Response to Salinity Stress
Time of Update: 2020-11-11
This chapter describes a protocol using oligo-microarrays to compare salinity treated (50 mM NaCl) and non-treated rice roots, presenting protocols for growth, RNA isolation, c DNA synthesis and labeling, and a summary of data collection, analysis, and interpretation.
Although focused on rice root tissue, the described procedures can be applied to many different treatments, tissues, and plant species.
-
Study on the trans-membrane transporting of different types of ECF transport proteins.
Time of Update: 2020-09-09
researchers from the Institute of Plant Physiology and Ecology of the Shanghai Institute of Life Sciences of the Chinese Academy of Sciences selected CbiMNQO, a typical representative of the specialized ECF transport protein, to carry out structural and physiological research on the subjects.
-
A new type of crystal structure of abc transporter LptB2FG membrane protein complex.
Time of Update: 2020-09-07
Huang Yihua's team in 2014 analyzed the high-resolution crystal structure of 2.4 E of the membrane protein complex LptD-LptE, which is responsible for transporting and assembling lipid polysaccharies on bacterial outer membranes, and initially clarified the molecular structure of how lipid polysaccharid molecules enter the outer membrane of bacteria (Nature, 2014).