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Preparation of Tris-hydrochloric acid buffer (0.05M, 25 degrees C).
Time of Update: 2020-10-28
Tris - hydrochloric acid buffer (0.05M , 25 degrees C) 50 ml 0.1M trihydroxymethymethane (Tris) solution mixed with X ml 0.1N hydrochloric acid, diluted to 100 ml.
pHX (ml) pHX (ml) 7.107.20.
0. The 1M solution is 12.114 g/L.
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Preparation of Tris buffers with various pH values of commonly used buffers.
Time of Update: 2020-10-20
Tris buffers with various pH values . the preparation of Tris buffers of various pHs required pH (181" 25 degrees C) 0.1mol/L HCl volume 7. 145. 77. 244. 77. 343. 47. 442. 07. 540. 37. 638. 57. 73
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1× method for the preparation of TATE buffer.
Time of Update: 2020-10-19
2. Add about 600mL of deionized water to the beech and stir to dissolve.
3. Add 57.1mL of acetic acid and stir well.
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Preparation method for PBS and D-PBS buffer solutions.
Time of Update: 2020-10-19
(2) D-PBS formula: sodium chloride 8 g, potassium chloride 0.2 g, phosphate d.15 g, potassium phosphate 0.2 g, calcium chloride 0.1 g, magnesium chloride containing 6 crystalline water 0.1 g dissolved in 1 L ddH2O.
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Preparation of phosphate buffer (PB).
Time of Update: 2020-10-17
1. A solution (0.2M sodium dihydrophosphate): NaH2 PO4 - H2 O 27.6g, dissolved in distilled water, diluted to 1000ml.
3. The preparation of different pH phosphate buffer (PB) buffers A liquid X ml (see table below), add B liquid Y ml, 0.2M petabytes.