polymerase assay

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Assay of mtDNA Polymerase from Human Tissues

 Time of Update: 2021-02-20

MitochondrialDNA (mtDNA) replication is a complex process involving over 20 proteins organized along the inner mitochondrial membrane as a multienzyme complex called the mtDNA replisome, or replication factory (1 –3 ). Figure 1 illustrates some of the protein components that participate in mitochondrial DNA replication.
Optimization of a Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Mass Assay for Low-Abundance mRNA

 Time of Update: 2020-12-25

Whereas Northern analysis is a standard procedure by which abundant levels of mRNA can be quantified and characterized by size, there is a limit to the sensitivity of this technique, even with the best probes and the use of poly(A+) mRNA enrichment ( see Chapters 28 and 30 ). At these times, it is necessary to move to more sensitive techniques.
Design and Optimization of Molecular Beacon Real-Time Polymerase Chain Reaction Assays

 Time of Update: 2020-12-10

This chapter describes the different steps necessary for establishing a molecular beacon real-time PCR assay: (1) target design, (2) primer design, (3) optimization of the amplification reaction conditions using SYBR Green, (4) molecular beacon design, and (5) molecular beacon synthesis and characterization.

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