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Pine (Pinus radiata)
Time of Update: 2021-01-15
This chapter describes the transformation ofPinus radiata using organogenic cotyledon explants rather than the more common somatic embryogenesis methods for conifers.
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DNA Extraction from Vegetative Tissue for Next-Generation Sequencing
Time of Update: 2021-01-15
In this chapter we describe a simple two-reagent DNA extraction protocol which yields a high quality and quantity of DNA which can be used for different applications including NGS.
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Tymovirus Isolation and Genomic RNA Extraction
Time of Update: 2021-01-15
The generic acronym, tymovirus, comes fromt urnip yellowmo saic virus (TYMV), the name of the first to be described ( 1 ), now the type species.
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Trichovirus Isolation and RNA Extraction
Time of Update: 2021-01-15
The genus Trichovirus, a newly established plant virus genus ( 1 ), contains five viral species (including three tentative members), with similar biological, morphological, physicochemical, and ultrastructural properties. Apple
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Tobravirus Isolation and RNA Extraction
Time of Update: 2021-01-14
The tobraviruses have a genome consisting of two positive-sense, single-stranded RNA molecules. The two genomic RNAs, RNA-1 and RNA-2, are encapsidated separately in rod-shaped particles with lengths of 180–215 nm (L particles) and 46–115 nm
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Ilarvirus Isolation and RNA Extraction
Time of Update: 2021-01-14
The genus Ilarvirus belongs to the familyBromoviridae , together with three other generaBromovirus, Cucumovirus , andAlfamovirus ( 1 ). The ilarvirus genus includes at present 15
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Geminivirus Isolation and DNA Extraction
Time of Update: 2021-01-13
Geminiviruses, named for their unique geminate capsid morphology, have small single-stranded (ss) circular DNA genomes that replicate in the nuclei of infected cells via a double stranded (ds) DNA intermediate. They are responsible
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Under the influence of the epidemic, the top ten trends in the plant extract market in 2021
Time of Update: 2021-01-12
. the impact on the nutrition and health industry under such a scenario? What are the future trends in the industry? What are the uncertainties and fluctuations? On December 17, the 15th Natural Extract Innovation and Development Forum
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Solid Phase Micro-Extraction GCMS Analysis of Natural Volatile Components in Melon and Rice
Time of Update: 2021-01-11
The natural fragrance compounds produced by plants play key roles in the long-term fitness and survival of these plants as well as being of direct/indirect benefit to man.
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Mitochondrial extraction and observation
Time of Update: 2021-01-08
and tissues , a large number of mitochondrials are extracted from these organ tissues, and when there are fewer samples used (e.g. observations of electronscopes and photonscopes) can be extracted from tissue cultured cells
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Extraction of chlorophyte pigment
Time of Update: 2021-01-08
containing water, so 95% alcohol and 80% acetone extraction, or with alcohol, acetone and water mixture. Chlorophylic pigment is susceptible to light oxidation, the extraction of pigment should be carried out in low light, and avoid light
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Supercritical Fluid Extraction and High Performance Liquid Chromatography Determination of Carbendazim in Bee Larvae
Time of Update: 2021-01-06
To control beehive treatment and establish the therapeutic dose for the product, it is compulsory to get reliable procedures to evaluate the residues of the fungicide on larvae.
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The Role of Inositol and the Principles of Labelling, Extraction, and Analysis of Inositides in Mammalian Cells
Time of Update: 2021-01-06
In this chapter, we establish a broad foundation for the rest of the book by covering some important principles of inositide methodologies.
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Extraction of RNA from Plants
Time of Update: 2021-01-06
Many of these plant products are not removed during conventional phenol extraction procedures, such as the one described previously in this series ( 1 ).
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Orange peel extract can be synthesized into polycarbonate (pictured)
Time of Update: 2021-01-05
, who has played an important role in synthesis, said: "There are few restrictions on the selective development of new PLimC-based materials.
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Extraction of Total RNA from Tissues and Cultured Cells
Time of Update: 2021-01-03
The rsolatron of intact, high quality, total cellular RNA is often the starting point for many molecular biologrcal procedures (Fig. 1 ) There are numerous general methods for the rsolation of total cellular RNA ( 1 – 9 ).
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Supercritical Fluid Extraction of Flumetralin from Tobacco Samples
Time of Update: 2021-01-03
These extractions may require several hours or even days to perform, use large volumes of ultrapure solvents, and often fail to yield quantitative extraction and recovery of target analytes.
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Application of Membrane Extraction with Sorbent Interface for Breath Analysis
Time of Update: 2021-01-02
The feasibility and validity of the MESI system was tested with real human breath samples.
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The Supercritical Fluid Extraction of Drugs of Abuse from Human Hair
Time of Update: 2021-01-02
Since 1979, hair has been used to document chronic human drug exposure ( 1 ).
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Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues
Time of Update: 2021-01-01
Thus the partitioning of leukotrienes C 4 , D 4 , and E 4 and their metabolites between these two phases differs from that of other eicosanoids.