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Capillary electrophoresis instrument-failure analysis seven
Time of Update: 2021-11-01
Possible cause: The buffer in the capillary flows out, causing current leakage .
Solution: Replace the capillary, and clean the optical fiber head and detection window with water .
Possible cause: The humidity in the experimental environment is too high .
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Capillary electrophoresis instrument-failure analysis eight
Time of Update: 2021-11-01
Possible cause: There is liquid at the neck of the bottle, which makes the stopper slippery .
Possible cause: There is a problem with the pressure system .
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Capillary electrophoresis instrument-failure analysis 6
Time of Update: 2021-11-01
Check result: Change the type of buffer, the peak shape and peak time are stable .
2. Inspection result: changing the type of buffer, the peak shape and peak time are still unstable .
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Capillary electrophoresis instrument-failure analysis 9
Time of Update: 2021-11-01
Inspection result: Re-cut the capillary inlet and the problem is solved .
Possible cause: The capillary inlet incision is not flat .
Solution: Re-cut the capillary inlet .
2. Inspection result: Re-cut the capillary inlet, the problem still exists .
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Determination method of glucocorticoid drug residue analysis technology-capillary electrophoresis
Time of Update: 2021-09-20
Li Boxiang and others used reversed-phase pCEC-UVD technology to establish an efficient and simple GCs analysis method, which is suitable for the detection of GCs in hair .
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Analysis of Environment-Induced DNA Damage by Capillary Electrophoresis
Time of Update: 2021-03-06
Using CE to detect small radiation-induced fragments of DNA and conformational changes in large DNA fragments and plasmids provides a faster and more efficient alternative to other techniques such as standard and pulsed-field agarose gel electrophoresis.
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On-Column Labeling Reaction for Analysis of Protein Contents of a Single Cell Using Capillary Electrophoresis With Laser-Induced Fluorescence Detection
Time of Update: 2021-03-05
This chapter presents methods for capillary electrophoresis (CE) fingerprinting of proteins in a cell extract and in single cells.
More than 20 relevant publications are cited in this chapter to assist the reader with adopting the presented methods.
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Glycoprotein Analysis by Capillary Zone Electrophoresis-Electrospray Mass Spectrometry
Time of Update: 2021-02-26
The study of glycoproteins as intact molecules or as smaller fragments generated by chemical or enzymatic digestion is wellsuited to the technique of capillary zone electrophoresis-electrospray mass spectrometry (CZE-ESMS).
In particular, CID of glycopeptides is characterized by fragment ions corresponding to cleavage at each glycosidic bond.
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Analysis of Fungicides in Fruits and Vegetables by Capillary Electrophoresis-Mass Spectrometry
Time of Update: 2021-02-15
A method based on solid-phase extraction (SPE) and capillary electrophoresis-mass spectrometry (CE-MS) for determining fungicides in fruits and vegetables is described.
The target compounds are isolated by solid-phase extraction (SPE) on C8, eluted with dichloromethane, evaporated to dryness, and reconstituted in buffer.
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Overview: The Application of Capillary Electrophoresis for DNA Polymorphism Analysis
Time of Update: 2021-01-06
Significant developments of other miniaturized electro-separation devices including molecular electrophoresis sieves and dielectric trapping using microelectrodes ( 13 , 14 ) have been described, which may be able to be integrated with CE to create micro-analytical or preparative devices.
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Analysis of Modified Oligonucleotides with Capillary Gel Electrophoresis
Time of Update: 2021-01-06
Short segments ofDNA , which have been synthesized chemically, are commonly known as synthetic oligonucleotides (ODNs).
Chemical modifications may be selectively placed at various locations within the molecule including the backbone, the heterocyclic base, or the sugar moiety ( 1 , 2 ).
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Amplification Refractory Mutation System Analysis of Point Mutations by Capillary Electrophoresis
Time of Update: 2021-01-04
The field of molecular diagnosis is evolving rapidly, and currently, it broadly comprises genetic disease analysis, paternitytesting, forensic studies, assessment of genetic risk, and so on.
A number of molecular techniques have been developed duringthe last 10–15 yr that greatly improve diagnostic and prognostic capabilities, allowing carrier detection, and prenatal diagnosis.
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Use of Capillary Electrophoresis for Concentration Analysis of Phosphorothioate Oligonucleotides
Time of Update: 2021-01-04
The rapid advance of phosphorothioate oligonucleotides in clinical development has accelerated the need for accurate quantitation of these compounds for quality control and bioanalytical applications ( 1 ).
This can be attributed to many factors including, superior resolution as a result of 10–100-fold higher electric fields, on line detection, and automation.
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High-Resolution Proteome/Peptidome Analysis of Body Fluids by Capillary Electrophoresis Coupled with MS
Time of Update: 2021-01-03
Here we provide protocolsfor sample preparation of various body fluids, namely, urine, plasma, and cerebrospinal fluid, and how to obtain maximum sensitivityand selectivity of the small proteins and peptides under investigation by CE-MS.
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Capillary Electrophoresis for the Analysis of Glycosaminoglycan-Derived Disaccharides
Time of Update: 2021-01-03
CE coupledto laser-induced fluorescence (LIF) detection shows an approximately 100 times higher sensitivity than traditional UV detectionat 232 nm.
Outlined in this chapteris a protocol of analyzing glycosaminoglycan-derived disaccharides by CE-LIF with AMAC derivatization.
