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Measurements of Ca2+ Concentration with Recombinant Targeted Luminescent Probes
Time of Update: 2021-03-04
The GFP-based Ca 2+ probes and aequorin are widely employed in the study of intracellular Ca 2+ homeostasis.
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Conductive coatings are paved with "guide zebra wire" instead of uneven guide bricks
Time of Update: 2021-03-04
", hope to have the opportunity to cooperate. Mingyue, director of education at the University of Hong Kong, led Gao's life, using cross-disciplinary imagination in the "Digital Reading" course to solve the problems that will be faced
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Measurement of Nitric Oxide-Related Enzymes in the Brain by In Situ Hybridization
Time of Update: 2021-03-04
We have developed a nonradioactive ISH protocol allowing the simultaneous coupling of immunohistochemistry to follow gene expression at the protein level on the same section as the mRNA detection.
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Methods for Measuring Class I Membrane-Bound Hyaluronan Synthase Activity
Time of Update: 2021-03-03
Three radioactive and nonradioactive assay methods are described that can give the amount of HA made with or without information about the distribution of product sizes.
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Measurement of Nitric Oxide Synthase Activity In Vivo and In Vitro by Gas Chromatography-Mass Spectrometry
Time of Update: 2021-03-02
Thus, the GC-MS nitrite/nitrate assay provides information about biologically relevant NOS activity.
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Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In
Time of Update: 2021-03-02
By combining a series of cell biological and biochemical approaches, and taking advantage of the enzyme DSN that specifically cuts double-stranded DNA and releases the telomeric overhangs, we have developed a method to monitor telomerase action during one cell cycle.
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Measurement of Calcium and Movement in Heart Cells
Time of Update: 2021-03-01
Preparations of calcium-tolerant cardiac myocytes for studies on intracellular calcium ([Ca 2+ ] i ) signaling and contraction have been difficult owing to the susceptibility of these cells to the enzymatic digestion process.
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applications of electroporation of adherent cellsIn Situ, on a partly conductive slide
Time of Update: 2021-03-01
This is applied in the presence of the fluorescent dye, Lucifer yellow, causing its penetration into the cells growing on the conductive half of the slide.
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Cancer cells use DNA replication error to 'live'
Time of Update: 2021-03-01
Specifically, the researchers began analyzing potential drivers of drug resistance by analyzing biopsy samples from cancer patients before and after receiving targeted cancer treatment.
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Guangxi six measures to strengthen the prevention and treatment of birth defects
Time of Update: 2021-02-28
the Programme has developed 16 specific indicators that are required for the prevention and treatment of birth defects at all stages of marriage, pregnancy, pregnancy, newborns and children.
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Sphingomyelin Mass Measurement
Time of Update: 2021-02-28
7) Vortex hard and spin samples at 3,000 rpm (in the tabletop centrifuge) for 5 minutes.
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Measurement of [3H]PN200-110 and [125I]-Conotoxin MVIIA Binding
Time of Update: 2021-02-27
In addition, many guanine nucleotide protein (G) coupled receptors couple to voltage-sensitive Ca 2+ channels to reduce Ca 2+ influx ( 9 , 10 ).
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Expanded Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)
Time of Update: 2021-02-27
Expanded newborn screening of inborn errors of metabolism (IEM) based on tandem mass spectrometry technology has emerged as one of the most successful preventative healthcare initiatives for presymptomatic diagnosis and treatment of rare yet treatable genetic diseases.
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The "sharp weapon" that captures the code of life can also measure whether the kebab is authentic
Time of Update: 2021-02-27
it is difficult to control liquid contamination, movement. In any case crude and inaccurate, in December 1983, Mullis saw the first PCR fragment of 49bp length after 10 cycles using isotope marking, a "0-1" breakthrough that made the PCR era.
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Measuring Ubiquitin Conjugation in Cells
Time of Update: 2021-02-26
Here, we describe in detail the steps to follow in order to determine whether a particular protein might become ubiquitinated, or deubiquitinated, and we offer warnings about pitfalls to avoid in antiubiquitin immunoblotting.
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Eur Radiol: Can the measurement of spleen volume evaluate the high risk of high-risk varicose veins in the compensation of viral cirrhosis B?
Time of Update: 2021-02-25
, it is recommended that patients with cirrhosis have a routine endoscopy to prevent the occurrence of varicose vein rupture bleeding. , however, more than half of patients with cirrhosis do not have gastroesoesopal varicose veins, resulting in a lower
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Measurement of Lipid Forces by X-Ray Diffraction and Osmotic Stress
Time of Update: 2021-02-25
Interlamellar spacing is determined by the balance of attractive van der Waals (charge fluctuation) and repulsive forces.
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Measurement of Receptor Endocytosis and Recycling
Time of Update: 2021-02-25
Numerous transmembrane receptors undergo clathrin-mediated endocytosis, followed by sorting in the early endosome.
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Measurement of Interleukins by ELISPOT Assay with Particular Application to Dual-Color Analysis (Stardust Assay)
Time of Update: 2021-02-24
However, this approach provides only cumulative amounts in a fixed time, thus limiting the sensitivity of the technique, particularly when relatively few cells are producing lymphokines of interest.
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The National Institutes of Health (NIH) conducted a study to assess the safety of Redsiway in COVID-19 pregnancy patients
Time of Update: 2021-02-24
study, called IMPAACT 2032, will be conducted by the International Network of Clinical Trials on Maternal, Child and Adolescent AIDS (IMPAACT) funded by the National Institutes of Health (NIH).
