In Vivo Analysis of Protein Translocation to the Escherichia coli Periplasm
Time of Update: 2021-03-04
Cleavable signal sequences can be used to monitor protein export in a time frame of 15–120 s.
Assay for a Galactosyltransferase Involved in the Assembly of the O7-Antigen Repeat Unit of Escherichia coli
Time of Update: 2021-03-03
The natural undecaprenol-linked acceptor substrates are not readily available, precluding the characterization of O-chain GTs. This chapter describes an assay for a galactosyltransferase (GalT) involved in the synthesis of the O7 antigen ofEscherichia coli VW187.
Application of a colorimetric assay to identify putative ribofuranosylaminobenzene 5-phosphate synthase genes expressed with activity inEscherichia coli
Time of Update: 2021-03-01
Given the importance of RFAP synthase in H 4 MPT biosynthesis, the identification of putative RFAP synthase genes and measurement of RFAP synthase activity would provide an indication of the presence of H4MPT in untested microorganisms.
The Hong Kong University of Science and Technology has cracked the carcinogenic mechanism of E. coli
Time of Update: 2021-02-28
coli is a genotoxic compound that destroys the double helix structure of DNA in ebony cells and increases the risk of colorectal cancer, according to researchers at the University of Science and Technology.
Automated Recombinant Protein Expression Screening in Escherichia coli
Time of Update: 2021-02-27
This chapter describes the automated procedure developed within the Structural Biology and Genomics Platform, Strasbourg for performing recombinant protein expression screening inEscherichia coli .
synthesis of sedition with E. coli
Time of Update: 2021-02-26
coli Sedent acid and succeeded in obtaining the Serotonin cell factory.
Generation of gene deletions and gene replacements in Escherichia coli O157:H7 using a temperature sensitive allelic exchange system
Time of Update: 2021-02-25
In this work we describe protocols for the generation of gene deletions and gene replacements using a temperature sensitive plasmid in Escherichia coli O157:H7.
of two new pathways for IAA microbial synthesis within E. coli cells
Time of Update: 2021-02-23
the beginning synthesis path, and finally recombinant strain MG-adhE::icd with glucose as the base, the resulting IAA yield increased to 906 mg/L. In this paper, E. coli as a cell factory exogenetic expression of IAM and TAM approach to the production
Neutralization of the In Vivo Activity of E. Coli-Derived Lipopolysaccharide by Cationic Peptides
Time of Update: 2021-02-22
Lipopolysaccharide-binding and neutralization of its biologic activity can be detected and quantified by monitoring specific in vivo responses induced by intravascular introduction of lipopolysaccharide into conscious, instrumented animals.
Escherichia coli Electrotransformation
Time of Update: 2021-02-21
With electrotransformation, as many as 80% of the cells receive exogenous DNA ( 1 ).
Effects of Estrogen Administration on the Colonization Capability of Lactobacilli and Escherichia coli in the Urinary Tracts of Mice
Time of Update: 2021-02-21
The first step in designing a probiotic product is to isolate and characterize strains with some beneficial properties.
Multiplex PCR for Specific Identification of Enterohemorrhagic Escherichia coli Strains in the O157:H7 Complex
Time of Update: 2021-02-20
The “O157:H7 complex” is comprised mostly of enterohemorrhagicEscherichia coli (EHEC) strains, with serotype O157:H7 being the prototypic and predominate pathogenic strain in the complex.
Engineering Escherichia coli for Canthaxanthin and Astaxanthin Biosynthesis
Time of Update: 2021-02-20
Here, we describe methods to assemble carotenoid biosynthesis operons from carotenogenic gene clusters isolated from different bacterial sources and evaluate canthaxanthin or astaxanthin production inE.
Use of Escherichia coli Mutator Cells to Mature Antibodies
Time of Update: 2021-02-20
Repeated rounds of mutation and increasingly stringent selection (Fig. 1 ) enable recovery of Abs of substantially elevated affinity for the target.
Transfer of Episomal and Integrated Plasmids from Saccharomyces cerevisiae to Escherichia coli by Electroporation
Time of Update: 2021-02-19
Adding telomere sequences to anARS/CEN plasmid creates a yeast artificial chromosome (YAC), which is a freely replicating, low copy number linear molecule ( 7 ).
Novel Approach in the Biosynthesis of Functional Carotenoids in Escherichia coli
Time of Update: 2021-02-19
coli cells that carry heterologous mevalonate pathway-based genes.
coli cells carrying only the lycopene-producing plasmid.
Detection of Verotoxin Genes VT 1 and VT 2 in Escherichia coli O157:H7 in Minced Beef Using Immunocapture and Real-Time PCR
Time of Update: 2021-02-18
Many outbreaks involving this pathogen have been linked to minced beef.
coli O157:H7 in minced beef.
Abundance in Sewage of Bacteriophages Infecting Escherichia coli O157:H7
Time of Update: 2021-02-17
in group A streptococci ( 1 ), the cholera toxin inVibrio cholerae ( 2 ), or enterotoxin production in enterohemorrhagic (EHEC) strains ofE. coli ( 3 ).
Sensitive and Rapid Detection of Campylobacter jejuni and Campylobacter coli Using Loop-Mediated Isothermal Amplification
Time of Update: 2021-02-15
From the beginning ofDNA extraction to final detectionof Campylobacter jejuni andCampylobacter coli , the assay requires less than 50 and 90 min from a colony on selective media, and human feces, respectively.
