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Analysis of Receptor Tyrosine Kinase Internalization Using Flow Cytometry
Time of Update: 2021-02-27
To understand how cMet signaling for normal cell growth is controlled by endocytosis and how these mechanisms are dysregulated in metastatic cancers, we developed flow cytometry-based assays to examine cMet internalization.
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High-temperature gas chromatography and gas chromatography-mass spectrometry of glycoprotein and glycosphingolipid oligosaccharides
Time of Update: 2021-02-27
High-temperature gas chromatography and gas chromatography-inass spectrometry for the analyses of oligosaccharides derived from glycoproteins or glycosphingolipids has been developed. Pcrmethylatcd oligosaccharides with up to about 12 sugar residues and masses up to 2500 Daltons can be analyzed. This approach is discussed and exemplified.
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Liquefied gas
Time of Update: 2021-02-26
These low-boiling gases are composed of carbon and hydrogen, both of which can burn.
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Examination of Bacillus anthracis Spores by Multiparameter Flow Cytometry
Time of Update: 2021-02-24
anthracis strains are fully virulent and the ability to determine the potential virulence of the endospore is also important.
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Chongqing sings about shale gas mining and commercial drama
Time of Update: 2021-02-23
industries to achieve the transformation and upgrading of Chongqing's old industrial base, this year's National "two sessions", members of the CPPCC from Chongqing put forward a collective proposal: to support Chongqing to build a national shale gas
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Guangxi: to open up the internal management flow barriers of the medical association
Time of Update: 2021-02-22
, Guangxi called for the integration of medical and health resources and the strengthening of capacity-building for primary health care services.
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Analysis of the DNA of Parasitic Protozoa by Flow Cytometry
Time of Update: 2021-02-20
Parasitic protozoa, generally, present as highly disperse popula tions with complex life cycles.
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In Situ Analysis of Chromatin Proteins During Development and Cell Differentiation Using Flow Cytometry
Time of Update: 2021-02-19
For instance, duringXenopus laevis early development, the midblastula transition (MBT) is characterized as a period after which the somatic replication-dependent type of H1 starts to accumulate and the embryonic H1 (B4) and HMG1 decrease dramatically ( 7 ).
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Flow Cytometric Techniques for Studying Plasmacytoid Dendritic Cells in Mixed Populations
Time of Update: 2021-02-19
Using surface and intracellular flow cytometry, phenotypic and functional aspects can be combined to accurately study PDCs in a mixed population of cells.
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Isolation of Carotenoid Hyperproducing Mutants of Xanthophyllomyces dendrorhous (Phaffia rhodozyma) by Flow Cytometry and Cell Sorting
Time of Update: 2021-02-19
Approaches for improving astaxanthin yields inXanthophyllomyces dendrorhous include optimization of fermentation conditions and generation of hyperproducing mutants through random mutagenesis using chemical or physical means.
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Enumeration of Bacteriophages Using Flow Cytometry
Time of Update: 2021-02-17
The present paper describes an optimized protocol for the enumeration of bacteriophages using a standard benchtop flow cytometer.
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Sampling Water Bodies: Tangential Flow Filtration
Time of Update: 2021-02-17
The study of microbial community structure via analysis of total communityDNA , or by the application of fluorescent oligonucleotide probes by fluorescentin situ hybridization, has become a valuable tool
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Viability of Amoebae, Fungal Conidia, and Yeasts: Rapid Assessment by Flow Cytometry
Time of Update: 2021-02-17
Rapid detection and viability measurements of microorganisms in homogenous and heterogenous microbial populations have been greatly enhanced by recent advances in the use of fluorescent stains in flow cytometry (FCM) ( 1 – 5 ).
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Flow Cytometry in Environmental Microbiology: A Rapid Approach for the Isolation of Single Cells for Advanced Molecular Biology Analysis
Time of Update: 2021-02-16
We describe here the combination of fluorescent in situ hybridization and cell sorting for the detection and isolation of Candidate Division TM7 bacteria from an enriched soil sample.
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Flow Cytometric Detection of Adenoviruses and Intracellular Adenovirus Proteins
Time of Update: 2021-02-14
Previous bioassays used to quantitate adenoviruses (such as the plaque assay or fluorescent focus assay) are time-consuming and subjective in their interpretation.
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Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid-Phase Microextraction-Gas Chromatography/Mass Spectrometry
Time of Update: 2021-02-13
Here, we describe a solid-phase microextraction-gas chromatography/mass spectrometry (SPME-GC/MS) analytical approach that identifies and analyzes volatile compounds in the headspace above a live fungal culture.
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Detection of Infectious Rotaviruses by Flow Cytometry
Time of Update: 2021-02-13
However, the sensitivity of these procedures is not high enough to detect the low number of viral particles sometimes present in the environment ( 3 ).
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Hebei Yantai Xindu District Outbreak Prevention and Control Flow Group: Do the "sharp soldiers" on the epidemic defense line
Time of Update: 2021-02-12
and Control Flow Working Group in Xindu District of Yantai City. 3rd, a new coronary pneumonia outbreak occurred in Yantai City. 6th, Yantai City launched the first round of full nucleic acid testing. 7, in order to effectively
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After 16 years in the industry painters quickly spray paint color uniform non-flow hanging
Time of Update: 2021-02-12
and service life. Paint should be regulated according to changes in weather and temperature. Badly adjusted furniture does not touch smooth, the surface has pinholes, easy to whiten. And paint according to the color of the customer to choose, some
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Automated Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry
Time of Update: 2021-02-12
The method for the determination of multiclass pesticides in water samples by headspace solid-phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC-MS) is described.
