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Agarose Gel Electrophoresis of Nucleic Acids
Time of Update: 2021-03-01
DNA fragments may be separated by gel electrophoresis in a gel composed of agarose.
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Mapping Epitopes on Antigens by Immunodiffusion in Gel
Time of Update: 2021-02-28
The method is based on the fact that a single MAb generates soluble immune complexes with an antigen, but two MAbs to different epitopes, when tested in appropriate conditions, generate insoluble complexes that are detected as a precipitin line.
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Staining of Preparative 2-D Gels: Coomassie Blue and Imidazole-Zinc Negative Staining
Time of Update: 2021-02-28
Fig. 1. Preparative 2-D gels stained by the imidazole-zinc negative stain (left) and colloidal Coomassie blue G-250 stain (right).
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Characterizing Proteins from 2-DE Gels by Internal Sequence Analysis of Peptide Fragments: Strategies for Microsample Handling
Time of Update: 2021-02-27
Considerable effort has also been expended to interface in situ digestion with micro-liquid chromotography (LC), peptide sequencers, and mass spectrometers.
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Single-Cell Gel Electrophoresis or Comet Assay of Intestinal Epithelial Cells Using Manual Scoring and Ridit Analysis
Time of Update: 2021-02-24
The single-cell gel electrophoresis or comet assay was initially reported in 1984 as a novel technique to directly visualize DNA damage in individual cells ( 1 ).
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Use of three-dimensional collagen gels to study mechanotransduction in t47d breast epithelial cells
Time of Update: 2021-02-24
This paper details several protocols we have developed for analyzing breast cell biology in 3D matrices, including culturing cells in 3D collagen gels, immunostaining cellular structures, and performing biochemical procedures directly from cells embedded in collagen gels.
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Analysis of Interleukin-2 Signaling Using Affinity Precipitations and Polyacrylamide Gel Electrophoresis
Time of Update: 2021-02-21
The γ- chain is shared with a number of other cytokines such as IL-3 and IL-7, which also play a role in cell growth ( 2 ).
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Application of Denaturing Gradient Gel Electrophoresis to Microbial Ecology
Time of Update: 2021-02-21
Denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) are techniques based on the separation of polymerase chain reaction ( PCR )-amplified gene fragments, not according to size, but owing to variation in the targeted nucleotide sequences.
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Detection and Molecular Epidemiology of Rotavirus by RNA Gel Electrophoresis
Time of Update: 2021-02-20
Electrophoretic separation of the segmented genome has gained popularity as a method not only for detection of rotavirus but also for molecular epidemiological studies (1 ,2 ).
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Pulsed Field Gel Electrophoresis for Mycobacteria
Time of Update: 2021-02-20
by a combination of new techniques for the extraction of intact chromosomalDNA in agarose blocks and electrophoresis using varying electrical fields.
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Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)
Time of Update: 2021-02-20
The application of molecular biology approaches holds the advantage of including culture-difficult and as-yet-uncultivated phylotypes in the profiles, providing a more comprehensive picture of the microbial community.
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Determination of Bacteriophage Genome Size by Pulsed-Field Gel Electrophoresis
Time of Update: 2021-02-17
Standard agarose gel electrophoresis is extensively used to resolveDNA fragments from 0.2 to 40–50 kb. Larger fragments of genomic DNA or whole viral genomes can only effectively be resolved by pulsed-field gel electrophoresis (PFGE), which extends the range of molecular separation from 200 bp to 12 Mb.
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White walnut gel is expected to be used as a food additive
Time of Update: 2021-02-15
Ingose extracted from processed vegetables such as walnuts and beets is expected to be a stable latex, while providing interesting new ingredients for emulsion-based foods.
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Gel and Substitutes (foodaily)
Time of Update: 2021-02-09
Albert Cianci , product line manager for Cargies North America's functional systems and hydrophobic collage, said, The first use of lemons, limes and oranges always follows the principle of 'fresh fruit first, peel second'.
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Fissin gel has been approved by the FDA for breakthrough therapy for low-risk upper urinary tract skin cancer
Time of Update: 2021-02-06
are delighted that UGN-101 has been designated as a breakthrough therapy with the potential to provide patients with less invasive organ retention therapies. We look forward to working with the FDA and preparing to send a new drug application (NDA) later
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Proteomic Analysis of Saliva: 2D Gel Electrophoresis, LC-MS/MS, and Western Blotting
Time of Update: 2021-01-31
Saliva harbors a wide spectrum of proteins that may reflect the health/disease status in the human body.
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Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates
Time of Update: 2021-01-24
The method involves running a first-dimension gel in order to separate restriction-digested replication intermediates of different mass.
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Cell: Heavy found that chromosomes are a gel that helps explain the spread of cancer
Time of Update: 2021-01-14
continue to overturn previous perceptions. , for example, on December 13, 2018, Harvard University's Adam Cohen, Shi Zheng, and others published a research paper in cell magazine entitled Cell Membranes Resist Flow (Cell Membrane Anti-Flow).
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Non-linear fluid behavior of non-versal emulsion gels made from natural supermolecule gels was studied by large amplitude oscillating shearing
Time of Update: 2021-01-12
experiments to study the nonlinear rheumogenic behavior of emulsion gel; osmotic emulsion gel has high elasticity and resistance to large deformation. The Food Protein and Collusion Laboratory, School of Food Science and Engineering, South
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Monrust sodium improvement new drug appears! The first external gel preparation was applied for clinical use
Time of Update: 2021-01-11
According to the patent statement, local preparations containing a combination of Monrust and mussel adhesive proteins can be used directly locally to treat inflammatory, inflammatory and/or inflammatory symptoms, including wounds, burns, psoriasis, acne and adiotic dermatitis.
