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    endothelial cells nitric oxide

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    • High-yield method for isolation and culture of endothelial cells from rat coronary blood vessels

      Time of Update: 2020-11-23
      The main advantages of our technique are: (i) good reproducibility, (ii) accurate sterility that can be maintained throughout the isolation procedure and (iii) high yield of pure endothelial cells, mainly due to microperfusion and temperature-controlled incubation with collagenase which allow an optimal distribution of this enzyme within the coronary vascular bed.

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