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Transient Gene Expression Analysis in Electroporated Maize Protoplasts
Time of Update: 2021-01-15
DirectDNA transformation is currently the method of choice for obtaining transformed cereals, such asZea mays , owing to the inherent host limitations ofAgrobacterium tumefaciens . This soil bacterium
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Blood: Fc-RIIA expression aggravates lupus nephritis and promotes plate plateboard resuscing
Time of Update: 2021-01-15
in a newly established mouse model based on plate activation of IC-Fc-RIIA mediated human SLE, bone marrow cell expression Fc-RIIA significantly aggravated lupus nephritis and accelerated mouse death.
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Transient Expression of Foreign Genes in Tissues of Arabidopsis thaliana by Bombardment-Mediated Transformation
Time of Update: 2021-01-15
Since the pioneering studies of Klein et al.
( 2 ) bombardment-mediated transformation has become a useful method for dehvermg foretgn genes mto plant cells ( 3 ).
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Transient Gene Expression in Medicago truncatula Leaves via Agroinfiltration
Time of Update: 2021-01-15
Transient expression is a powerful method for the functional characterization of genes. In this chapter, we outline a protocol for the transient expression of constructs inMedicago truncatula leaves usingAgrobacterium
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Xenopus Oocytes as a Heterologous Expression System
Time of Update: 2021-01-15
Oocytes of the South African clawed toad,Xenopus laevis , have proved a versatile and powerful heterologous expression system for eukaryotic genes ( 1 ). Xenopus oocytes were first used to express plant
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Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections
Time of Update: 2021-01-15
The use of more diverse methods to study gene function in plants is increasing the probability of successfully identifying and characterizing host factors affecting virus replication in plant systems.
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Transgenic Plants Expressing Toxins from Bacillus thuringiensis
Time of Update: 2021-01-14
In conventional plant breeding, one must usually find the trait of interest within the same or closely related plant species.
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Purification of Rubisco Activase from Leaves or after Expression in Escherichia coli
Time of Update: 2021-01-14
Rubisco activase is a molecular chaperone that modulates the activation state of Rubisco by catalyzing the ATP-dependent removal of tightly-bound inhibitory sugar-phosphates from Rubisco’s catalytic sites. This chapter reports methods developed for the purification of native and recombinant Rubisco activase from leaves and bacterial cells, respectively.
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Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues
Time of Update: 2021-01-14
In this chapter, we discuss specific considerations for studying gene expression by using qRT-PCR, such as the identification of suitable reference genes for the experimental setup used.
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Screening for Bioactive Small Molecules by In Vivo Monitoring of Luciferase-Based Reporter Gene Expression in Arabidopsis thaliana
Time of Update: 2021-01-13
Thereby screening for small molecules affecting different signaling pathways and/or phenotypic responses is possible.
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Heterologous Expression in Budding Yeast as a Tool for Studying the Plant Cell Morphogenesis Machinery
Time of Update: 2021-01-13
participating in cell morphogenesis; outline the strategies of experiment planning, yeast strain selection (or construction), and expression vector choice; and provide detailed protocols for yeast transformation, transformant selection, and phenotype evaluation.
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Expression Library Screening
Time of Update: 2021-01-08
When trying to identify a clone within a c DNA library, which may contain a coat protein (CP) gene, one useful technique may by immunological screening, using antibodies raised against either purified virus or isolated CP.
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"Pharmaceutical Express" Jiangsu announced the new crown vaccine procurement catalog MSD sell-off Mod...
Time of Update: 2021-01-07
December 3, 2020 / Medical Information List: BMS Odivo New Adaptive Disease Listing Application was accepted by CDE; Merca East sold Moderna shares; Henrenoli glycopeptide injection clinical trial approved; Stone Pharmaceutical Group "first
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Comparative Quantitation of mRNA Expression in the Central Nervous System Using Fluorescence In Situ Hybridization
Time of Update: 2021-01-07
It has been difficult to measure relative changes in gene expression within these regions because of the poor cellular resolution afforded by radioactively labeled probes and problems associated with densitometric analysis by counting silver grain deposition.
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RNA Probes for the Analysis of Gene Expression
Time of Update: 2021-01-06
Third, the enzymes are highly processive, allowing the efficient synthesis of very long transcripts from DNA templates.
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"Pharmaceutical Express" Astellas reached a $600 million partnership for the first domestic P...
Time of Update: 2021-01-06
11, Merca East announced that the European Medicines Agency CHMP on the new adaptation of K drug received positive advice, recommended the approval of Keytruda as a microsatellite highly unstable or mismatched repair defective colorectal cancer
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Immunological Detection of Gene Expression in Recombinant Clones
Time of Update: 2021-01-06
This may be applicable in primary cloning if no specificDNA probe is available, but is more generally used in subsequent studies of expression of specific foreign genes.
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Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes
Time of Update: 2021-01-06
The transfer or “transfectron” of genetic material into tissue-culture cell lines and the subsequent analysis of its RNA andprotein products, has become a key and central technology in contemporary cell biology, molecular biology, and protein chemtstry. Transfectron of genes provides a functronal end-point assay for experiments addressing a number of is sues including
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"Pharmaceutical Express" and Platinum Pharmaceuticals listed breaking AbbVie JAK inhibitors up to Phase 3...
Time of Update: 2021-01-06
interest in related antibody products. this is also the fourth antibody project developed by Acus, a pharmaceutical company. (A.T.) Saishang Pharma completed a $100 million round of A-round financing on December 8, Saisan Pharma announced
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Expression of Mammalian Cytochromes P450 in Yeast
Time of Update: 2021-01-05
Recent extensive research on cytochrome P450 (P450) has clarified that P450 constitutes a superfamily of hemoproteins, which are believed to have evolved from a common ancestor. The versatility of P450 is mainly owing to the existence
