Purification of Mitochondrial Uracil-DNA Glycosylase Using Ugi-Sepharose Affinity Chromatography
Time of Update: 2021-02-22
Separation is achieved through a highly specific, but reversible, interaction with a complementary binding substrate (ligand) that is immobilized on a solid support (matrix).
Genomic DNA Affinity Chromatography: A Technique to Isolate Interferon-Inducible DNA Binding Factors
Time of Update: 2021-01-31
This procedure permits the identification of IFN-inducible STAT complexes as well as any other IFN-inducible DNA binding factors.
An Overview of Affinity Chromatography
Time of Update: 2021-01-06
Having a new book on this methodology is eminently appropriate today.
And being able to introduce this book is our pleasure.
Dye-Ligand Affinity Chromatography for Protein Separation and Purification
Time of Update: 2020-12-29
The main disadvantage of reactive chlorotriazine dyes appears to be their moderate selectivity, which may limit their use.
Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography
Time of Update: 2020-12-25
The use of a series of immobilized lectin columns, whose sugar-binding specificities have been precisely elucidated, enables us to fractionate a very small amount of radioactive oligosaccharides or glycopeptides (∼10 ng depending on the specific activity) into structurally distinct groups.
The Purification of Poly(A)-Containing RNA by Affinity Chromatography
Time of Update: 2020-12-18
These commercially available materials consist of polymers of about 10–20 nucleotides, covalently bound to a carbohydrate support, and bind RNA containing a poly(A) tract as short as 20 residues.
with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-Step Lectin Affinity Chromatography
Time of Update: 2020-12-12
This protocol describes Zinc finger nuclease gene targeting of human cells to simplify the glycoproteome, as well as lectin chromatography and isolation of glycopeptides from total cell lysates of SimpleCells.
Isolation of mRNA by Affinity Chromatography
Time of Update: 2020-12-10
Messenger RNA (mRNA) comprises approximately 1–5% of total cellular RNA. Although the actual amount depends on the type of cell and its physiological state, at any one time approximately 12,000 genes are being transcribed with approximately
Affinity Perfusion Chromatography
Time of Update: 2020-12-07
This solvent pool leads to slow mass transport to interior binding sites, necessitating relatively low operating flow rates and long cycle times ( 30 – 33 ).
Affinityography (Affinity Chromatography)
Time of Update: 2020-11-03
It has long been recognized that proteins, enzymes and other biological large molecular substances can be combined with certain corresponding molecules single-specific and reversible, can be used for the separation and purification of biological molecules.