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Purification of Mitochondrial Uracil-DNA Glycosylase Using Ugi-Sepharose Affinity Chromatography
Time of Update: 2021-02-22
Separation is achieved through a highly specific, but reversible, interaction with a complementary binding substrate (ligand) that is immobilized on a solid support (matrix).
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Genomic DNA Affinity Chromatography: A Technique to Isolate Interferon-Inducible DNA Binding Factors
Time of Update: 2021-01-31
This procedure permits the identification of IFN-inducible STAT complexes as well as any other IFN-inducible DNA binding factors.
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An Overview of Affinity Chromatography
Time of Update: 2021-01-06
Having a new book on this methodology is eminently appropriate today.
And being able to introduce this book is our pleasure.
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Dye-Ligand Affinity Chromatography for Protein Separation and Purification
Time of Update: 2020-12-29
The main disadvantage of reactive chlorotriazine dyes appears to be their moderate selectivity, which may limit their use.
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Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography
Time of Update: 2020-12-25
The use of a series of immobilized lectin columns, whose sugar-binding specificities have been precisely elucidated, enables us to fractionate a very small amount of radioactive oligosaccharides or glycopeptides (∼10 ng depending on the specific activity) into structurally distinct groups.
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The Purification of Poly(A)-Containing RNA by Affinity Chromatography
Time of Update: 2020-12-18
These commercially available materials consist of polymers of about 10–20 nucleotides, covalently bound to a carbohydrate support, and bind RNA containing a poly(A) tract as short as 20 residues.
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with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-Step Lectin Affinity Chromatography
Time of Update: 2020-12-12
This protocol describes Zinc finger nuclease gene targeting of human cells to simplify the glycoproteome, as well as lectin chromatography and isolation of glycopeptides from total cell lysates of SimpleCells.
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Isolation of mRNA by Affinity Chromatography
Time of Update: 2020-12-10
Messenger RNA (mRNA) comprises approximately 1–5% of total cellular RNA. Although the actual amount depends on the type of cell and its physiological state, at any one time approximately 12,000 genes are being transcribed with approximately
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Affinity Perfusion Chromatography
Time of Update: 2020-12-07
This solvent pool leads to slow mass transport to interior binding sites, necessitating relatively low operating flow rates and long cycle times ( 30 – 33 ).
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Affinityography (Affinity Chromatography)
Time of Update: 2020-11-03
It has long been recognized that proteins, enzymes and other biological large molecular substances can be combined with certain corresponding molecules single-specific and reversible, can be used for the separation and purification of biological molecules.
