Escherichia coli has been studied for many years by two-dimensional polyacrylamide gel electrophoresis (2-D gels) ( 1 , 2 ). This method has provided much information about the physiology ofE. coli
Preparation of RNA Gel Blots
Time of Update: 2021-03-04
Electrophoresis of RNA through agarose gels for blotting requires complete denaturation of the RNA.
and diagonal sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Time of Update: 2021-03-02
Efficient protein folding and quality control are essential for unperturbed cell viability.
Agarose Gel Electrophoresis of Nucleic Acids
Time of Update: 2021-03-01
DNA fragments may be separated by gel electrophoresis in a gel composed of agarose.
Mapping Epitopes on Antigens by Immunodiffusion in Gel
Time of Update: 2021-02-28
The method is based on the fact that a single MAb generates soluble immune complexes with an antigen, but two MAbs to different epitopes, when tested in appropriate conditions, generate insoluble complexes that are detected as a precipitin line.
Staining of Preparative 2-D Gels: Coomassie Blue and Imidazole-Zinc Negative Staining
Time of Update: 2021-02-28
Fig. 1. Preparative 2-D gels stained by the imidazole-zinc negative stain (left) and colloidal Coomassie blue G-250 stain (right).
Characterizing Proteins from 2-DE Gels by Internal Sequence Analysis of Peptide Fragments: Strategies for Microsample Handling
Time of Update: 2021-02-27
Considerable effort has also been expended to interface in situ digestion with micro-liquid chromotography (LC), peptide sequencers, and mass spectrometers.
Single-Cell Gel Electrophoresis or Comet Assay of Intestinal Epithelial Cells Using Manual Scoring and Ridit Analysis
Time of Update: 2021-02-24
The single-cell gel electrophoresis or comet assay was initially reported in 1984 as a novel technique to directly visualize DNA damage in individual cells ( 1 ).
Use of three-dimensional collagen gels to study mechanotransduction in t47d breast epithelial cells
Time of Update: 2021-02-24
This paper details several protocols we have developed for analyzing breast cell biology in 3D matrices, including culturing cells in 3D collagen gels, immunostaining cellular structures, and performing biochemical procedures directly from cells embedded in collagen gels.
Application of Denaturing Gradient Gel Electrophoresis to Microbial Ecology
Time of Update: 2021-02-21
Denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) are techniques based on the separation of polymerase chain reaction ( PCR )-amplified gene fragments, not according to size, but owing to variation in the targeted nucleotide sequences.
Analysis of Interleukin-2 Signaling Using Affinity Precipitations and Polyacrylamide Gel Electrophoresis
Time of Update: 2021-02-21
The γ- chain is shared with a number of other cytokines such as IL-3 and IL-7, which also play a role in cell growth ( 2 ).
Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)
Time of Update: 2021-02-20
The application of molecular biology approaches holds the advantage of including culture-difficult and as-yet-uncultivated phylotypes in the profiles, providing a more comprehensive picture of the microbial community.
Pulsed Field Gel Electrophoresis for Mycobacteria
Time of Update: 2021-02-20
by a combination of new techniques for the extraction of intact chromosomalDNA in agarose blocks and electrophoresis using varying electrical fields.
Detection and Molecular Epidemiology of Rotavirus by RNA Gel Electrophoresis
Time of Update: 2021-02-20
Electrophoretic separation of the segmented genome has gained popularity as a method not only for detection of rotavirus but also for molecular epidemiological studies (1 ,2 ).
Determination of Bacteriophage Genome Size by Pulsed-Field Gel Electrophoresis
Time of Update: 2021-02-17
Standard agarose gel electrophoresis is extensively used to resolveDNA fragments from 0.2 to 40–50 kb. Larger fragments of genomic DNA or whole viral genomes can only effectively be resolved by pulsed-field gel electrophoresis (PFGE), which extends the range of molecular separation from 200 bp to 12 Mb.
White walnut gel is expected to be used as a food additive
Time of Update: 2021-02-15
Ingose extracted from processed vegetables such as walnuts and beets is expected to be a stable latex, while providing interesting new ingredients for emulsion-based foods.
