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Preparation of Direct, Enzyme-Labeled DNA Probes
Time of Update: 2021-03-02
The methodology was combined with enhanced chemiluminescence ( 2 ) (a light-producing HRP catalyzed reaction based on luminol) ( see Chapter 26 ) allowing the detection of specific hybrids on membranes ( 3 ).
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Enhanced DNA transfer into fish bone cells using polyethylenimine
Time of Update: 2021-03-02
Its efficiency in transferring DNA intoa wide range of cell types, including non-mammalian and hard-to-transfect cells, in addition to a low cost, show that PEI is a reagent of choice for nonviral vector transfection.
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Indian scientists have found that certain areas of DNA are protected from radiation
Time of Update: 2021-03-02
researchers say the results show that the G-four-strand structure avoids radiation-induced DNA fractures and is more resistant to radiation, and that G-four-stranded DNA is an important factor in the differences in radiation sensitivity in the human genome.
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UV-Induced DNA Damage and DNA Repair in Ribosomal Genes Chromatin
Time of Update: 2021-03-02
nucleosomes rDNA, that the formation of CPDs promotes changes in the active rDNA chromatin, and that NER is coupled to rRNA genes transcription.
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Advances have been made in the study of tumor vaccines based on DNA nanoma machines
Time of Update: 2021-03-02
feature a programmeable design that further optimizes the delivery of functional components such as virus-related antigens and ad adhesives, promising to provide a new platform for building antiviral vaccines. (National Nano-Center)
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Relationship between ultra-short DNA low methylation region and chromatin 3D structure
Time of Update: 2021-03-02
By developing novel calculation methods, this study detects the functional single base sparse conservative low methylation CpG (scUMC) and integrates the chromatin advanced structural data to analyze its potential regulatory functions in depth.
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Determination of the molecular weight of DNA-binding proteins using UV-crosslinking and SDS-PAGE
Time of Update: 2021-03-01
Following irradiation with a UV light source, the DNA/protein complex is subjected to SDS-PAGE and its molecular weight determined by comparison with appropriate protein standards.
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One-Step One-Lane Chemical Sequencing of DNA
Time of Update: 2021-03-01
There are several chemical methods for sequencing DNA . The classical method, introduced in 1977 by Maxam and Gilbert ( 1 ), uses four different chemical reactions to attack, modify, and open the heterocyclic bases
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Cancer cells use DNA replication error to 'live'
Time of Update: 2021-03-01
Specifically, the researchers began analyzing potential drivers of drug resistance by analyzing biopsy samples from cancer patients before and after receiving targeted cancer treatment.
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Enzyme-Labeled DNA Probes
Time of Update: 2021-03-01
The presence of certain types of human papillomavirus in cervical smears can be used as an indicationof neoplasia ( 2 ).
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How tumor metabolites block DNA repair
Time of Update: 2021-03-01
Recently, the journal Nature revealed how several metabolites that accumulate at higher levels in tumor cells inhibit the mechanisms of DNA repair, suggesting a direct link between metabolic changes and genomic instability caused by DNA damage.
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Detection of DNA Sequences Using Biotinylated Probes
Time of Update: 2021-02-28
Figure 1 shows the structure of biotin-11-dUTP, a commonly used substrate for biotinylation by the method of nick translation (2 ).
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A novel method for preparing single-stranded DNA for pyrosequencing
Time of Update: 2021-02-28
In this article, an enzymatic liquid-phase method for the generation of high-quality, single-stranded DNA, and its novel use for Pyrosequencing™ are described.
The method has also been fully automated.
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Our country has built the first DNA database of volunteer martyrs
Time of Update: 2021-02-28
identified the remains of unknown volunteer martyrs through DNA technology. Reporters learned on the 13th, to undertake this task is military medical research institute Wang Shengqi team. They broke through international challenges
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Repairing human DNA damage Scientists have found new clues in plants
Time of Update: 2021-02-28
, including us humans, are mutated daily by ultraviolet radiation, freekids and other chemicals, causing damage to the DNA of genetic material in the body. In the process of REPAIRing DNA damage, a cross-shaped DNA connection, the Hollidi
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Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes
Time of Update: 2021-02-28
The identification of specific DNA fragments is possible since their relative positions are maintained during the transfer from the gel to the filter.
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DNase I Footprinting of Small Molecule Binding Sites on DNA
Time of Update: 2021-02-27
DNase I is a convenient endonuclease for detecting and locating the position of actinomycin-binding sites within GC-rich sequences.
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DNA Rescue by the Vectorette Method
Time of Update: 2021-02-27
Several methods have been used to this end, includingPCR with vector-specific primers in combination with primers designed either for repetitive elements, such asAlu sequences ( 3 ), or in combination with random nonspecific primers ( 4 ).
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Cao Xuetao and others found the first in-cell DNA immunometric recognition subject
Time of Update: 2021-02-26
the early hours of July 19th, the latest issue of Science published online the papers of Cao Xuetao, a member of the Chinese Academy of Engineering and president of Nankai University, in the form of a long-form study. According
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Chinese scientists "draw" the most genome-wide map of soybeans
Time of Update: 2021-02-26
through the traditional linear genome storage form, the first time in the plant to achieve the construction of a graphical structure-based genome, will lead a new generation of genomics research ideas and methods, the reviewers called "the milestone
