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Measuring Soluble Ion Concentrations (Na+, K+, Cl) in Salt-Treated Plants
Time of Update: 2021-03-07
The maintenance of a high uptake of K + is also essential, so measurements of Na + , K + or Cl − are frequently used to screen for genetic variation in salinity tolerance.
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The General Administration of Market Supervision issued the Measures for the Administration of the Approval of Biological Products
Time of Update: 2021-03-07
consist of eight chapters and 48 articles, the main amendments include: First, to clarify the division of responsibilities for the issuance of issues and the duties of the issuing agencies, and improve the procedures
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Measuring solution viscosity and its effect on enzyme activity
Time of Update: 2021-03-07
According to Kramers theory, solvent viscosity results in friction against proteins in solution, and this should result in decreased motion, inhibiting catalysis in motile enzymes.
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Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR)
Time of Update: 2021-03-06
To a large extent, the advent of fluorescent indicators of free calcium ion concentration that could be loaded into cells in a nondisruptive manner has been responsible for our current knowledge of cellular calcium homeostasis ( 2 ).
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Measurement of S-Glutathionated Hemoglobin in Human Erythrocytes by Isoelectric Focusing Electrophoresis
Time of Update: 2021-03-06
Because an increase in cellular glutathione (GSSG) level is correlated with the formation of S-glutathionated protein, a mixed-disulfide exchange reaction has been proposed to be responsible for this protein modification ( 2 – 5 , 9 – 10 ).
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Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration
Time of Update: 2021-03-05
TIRF is particularly powerful when combined with electrophysiology so that ion channel activity can be related simultaneously to the local subplasma membrane and bulk average [Ca 2+ ] c .
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Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells
Time of Update: 2021-03-05
In this chapter, I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential.
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Calcium Measurements from Whole Heart Using Rhod-2
Time of Update: 2021-03-05
Here, we describe the optical apparatus and the method to record intracellular calcium transients.
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Measurements of Ca2+ Concentration with Recombinant Targeted Luminescent Probes
Time of Update: 2021-03-04
The GFP-based Ca 2+ probes and aequorin are widely employed in the study of intracellular Ca 2+ homeostasis.
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Measurement of Nitric Oxide-Related Enzymes in the Brain by In Situ Hybridization
Time of Update: 2021-03-04
We have developed a nonradioactive ISH protocol allowing the simultaneous coupling of immunohistochemistry to follow gene expression at the protein level on the same section as the mRNA detection.
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Methods for Measuring Class I Membrane-Bound Hyaluronan Synthase Activity
Time of Update: 2021-03-03
Three radioactive and nonradioactive assay methods are described that can give the amount of HA made with or without information about the distribution of product sizes.
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Measurement of Nitric Oxide Synthase Activity In Vivo and In Vitro by Gas Chromatography-Mass Spectrometry
Time of Update: 2021-03-02
Thus, the GC-MS nitrite/nitrate assay provides information about biologically relevant NOS activity.
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Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In
Time of Update: 2021-03-02
By combining a series of cell biological and biochemical approaches, and taking advantage of the enzyme DSN that specifically cuts double-stranded DNA and releases the telomeric overhangs, we have developed a method to monitor telomerase action during one cell cycle.
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Measurement of Calcium and Movement in Heart Cells
Time of Update: 2021-03-01
Preparations of calcium-tolerant cardiac myocytes for studies on intracellular calcium ([Ca 2+ ] i ) signaling and contraction have been difficult owing to the susceptibility of these cells to the enzymatic digestion process.
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Guangxi six measures to strengthen the prevention and treatment of birth defects
Time of Update: 2021-02-28
the Programme has developed 16 specific indicators that are required for the prevention and treatment of birth defects at all stages of marriage, pregnancy, pregnancy, newborns and children.
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Sphingomyelin Mass Measurement
Time of Update: 2021-02-28
7) Vortex hard and spin samples at 3,000 rpm (in the tabletop centrifuge) for 5 minutes.
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Measurement of [3H]PN200-110 and [125I]-Conotoxin MVIIA Binding
Time of Update: 2021-02-27
In addition, many guanine nucleotide protein (G) coupled receptors couple to voltage-sensitive Ca 2+ channels to reduce Ca 2+ influx ( 9 , 10 ).
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The "sharp weapon" that captures the code of life can also measure whether the kebab is authentic
Time of Update: 2021-02-27
it is difficult to control liquid contamination, movement. In any case crude and inaccurate, in December 1983, Mullis saw the first PCR fragment of 49bp length after 10 cycles using isotope marking, a "0-1" breakthrough that made the PCR era.
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Measuring Ubiquitin Conjugation in Cells
Time of Update: 2021-02-26
Here, we describe in detail the steps to follow in order to determine whether a particular protein might become ubiquitinated, or deubiquitinated, and we offer warnings about pitfalls to avoid in antiubiquitin immunoblotting.
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Measurement of Lipid Forces by X-Ray Diffraction and Osmotic Stress
Time of Update: 2021-02-25
Interlamellar spacing is determined by the balance of attractive van der Waals (charge fluctuation) and repulsive forces.
