-
Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format
Time of Update: 2020-12-03
Historically, the identification of such organisms has been done using conventional culture methods and biochemical techniques.
-
DNA Damage by Carbon Nanotubes Using the Single Cell Gel Electrophoresis Technique
Time of Update: 2020-12-01
In this technique, cells are embedded in agarose, lysed, subjected to electrophoresis, and stained with a fluorescent DNA-binding dye.
-
RNA Electrophoretic Mobility Shift Assay Using a Fluorescent DNA Sequencer
Time of Update: 2020-11-29
In this method, RNA was prepared by in vitro transcription using Texas Red-labeled nucleotides.
-
A Sensitive Sandwich DNA Array Using Fluorescent Nanoparticle Probes
Time of Update: 2020-11-26
An ultrasensitive sandwichDNA array using highly fluorescent and photostable dye-doped silica nanoparticles is described.Compared to traditional sandwich arrays in which fluorophores have been used to signal target DNA molecules, the developednanoparticle probes provide a much stronger fluorescent emission.
-
Radiolabeling of DNA Using Polynucleotide Kinase
Time of Update: 2020-11-26
Labeling ofDNA fragments at the 5′ end with polynucleotide kinase is one of the methods currently employed to obtain highly labeled DNA for sequencing purposes. This end-labeled DNA is used in the partial chemical degradation method for sequencing DNA(see Chapter 51 and ref. 1 ).
-
Synthesis of DNA Using a New Two-Step Cycle
Time of Update: 2020-11-26
For the first time a new, two-step method is described for synthesizing deoxyribonucleic acid. The approach uses 5′-carbonate protected 2′-deoxynucleoside-3′-phosphoramidites as synthons and a peroxy anion buffer that removes the carbonate
-
S1 Mapping Using Single-Stranded DNA Probes
Time of Update: 2020-11-23
In addition, it digests partially mismatched double-stranded molecules with such sensitivity that even a single base-pair mismatch can be cut and hence detected.
-
Functional Characterization of Rice Genes Using a Gene-Indexed T-DNA Insertional Mutant Population
Time of Update: 2020-11-20
Gene-indexed mutants with flanking sequences around inserted T-DNA are valuable resources for accelerating functional characterizations of rice genes.
-
Analysis of Mutation/Rearrangement Frequencies and Methylation Patterns at a Given DNA Locus Using Restriction Fragment Length Polymorphism
Time of Update: 2020-11-20
Since DNA can be digested with methylation sensitive enzymes, the assay can also be used to analyze a methylation pattern of a particular locus.
-
Construction of Infectious Clones of Double-Stranded DNA Viruses of Plants Using Citrus Yellow Mosaic Virus as an Example
Time of Update: 2020-11-06
Double-strandedDNA (dsDNA) viruses of plants are believed to be plant pararetroviruses.
-
Electroporation of Tobacco Leaf Protoplasts Using Plasmid DNA or Total Genomic DNA
Time of Update: 2020-11-05
For example, the strictly seed-specific gene encoding soybean β-conglycinin is expressed in tobacco mesophyll protoplasts, but the sequences responsible for that expression are different from those controlling expression in stable-transformation experiments ( 10 ).
-
Tips for using the legal amount of vitamin B2 in fluorescent phosphorescence
Time of Update: 2020-11-01
970CRT fluorescence photophoometer (Shanghai Analysis Instrument Factory) 5 mL straw 1, 2 mL straw 1, 50 mL bottle 7 10.0-mL-1VB2 standard solution (stored in the dark), ice acetic acid (AR), multidimensional glucose powder sample c.
-
DNA probes mark the preparation of commonly used reagents.
Time of Update: 2020-10-26
(16) TE buffer (Tris/EDTA): Tris, 10mmol/l pH7.4 (0.5ml 2mol/L storage), 1.0mmol/l ED-TA, pH8.0 (20 sl 0.5mol/L) storage, plus water to 100ml, room temperature.
-
The most commonly used tool enzyme in DNA recombination techniques.
Time of Update: 2020-10-26
DNA restrictive endoenzymes sticky ends are paired and recombined sexual cleavage enzymes and there are some different sources of restriction enzymes that identify the same nucleotide target sequence, which is called isolytic enzymes.
-
Tips for using art wall paint.
Time of Update: 2020-10-05
yellow , the most distinctive color, can give a warm feeling, because it is as prominent and eye-catching as red, so the excessive use of yellow on large areas of items, may make people restless.
-
The use of "Internet Plus", big data and other technical means - "food security" such as guarding the tip of the tongue security.
Time of Update: 2020-09-29
supervision system, etc. have made preliminary exploration, and achieved some results. February 3, the Executive Meeting of the State Council adopted the "13th Five-Year Plan" national food safety plan clearly, to strengthen the whole process
-
Nanchang agricultural products wholesale market is now using "a glass of citric acid" white lotus.
Time of Update: 2020-09-06
on the lotus. in another shop, the reporter saw a wholesale boss also sprinkled the same crystal on the lotus. The boss said it would be much better to have this crystal lotus. Reporters noted that the crystal particles in the red basin
-
DNA nanotechnology: Use information in DNA to build objects.
Time of Update: 2020-08-28
example, GATTAquant DNA Nanotechnology, based in Brunswick, Germany, uses DNA origami structures and fluorescent molecules to make nanoscales to validate ultra-high resolution microscopes.
-
Ultra-precision DNA compilation technology can also be used in biological weapons in addition to attacking human cancer cells.
Time of Update: 2020-08-26
Sotos, Intel's chief medical officer, points out that effective cancer treatment requires ultra-sophisticated DNA codification technology that can be used to overcome the challenges of developing biological weapons.
-
German media: Scientists use the DNA of glowing mushrooms to invent bioluminescent plants.
Time of Update: 2020-08-24
we didn't expect to see mature plants glow with the naked eye," Sargsan said in a statement.
