Tips | In addition to pressurizing and oxygenating, this trick will also get immediate results when laryngospasm is used
Time of Update: 2021-03-27
>Laryngeal spasm is the body's protective reflex to prevent foreign bodies from invading. It is a functional respiratory obstruction. Although
Generation of gene deletions and gene replacements in Escherichia coli O157:H7 using a temperature sensitive allelic exchange system
Time of Update: 2021-02-25
In this work we describe protocols for the generation of gene deletions and gene replacements using a temperature sensitive plasmid in Escherichia coli O157:H7.
Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System
Time of Update: 2021-02-22
Mobile generic elements are discreteDNA fragments encoding their own transposltlon functions, which enable them to move from one site in the DNA to another, independently of host-recombination functions (such as RecA).
Sensitive and Rapid Detection of Campylobacter jejuni and Campylobacter coli Using Loop-Mediated Isothermal Amplification
Time of Update: 2021-02-15
From the beginning ofDNA extraction to final detectionof Campylobacter jejuni andCampylobacter coli , the assay requires less than 50 and 90 min from a colony on selective media, and human feces, respectively.
The Use of Temperature-Sensitive Plasmids in Mycobacteria
Time of Update: 2021-02-01
The construction of allelic exchange mutants is one of the major strategies to decipher the function of a defined gene.
Sensitive and Rapid Detection of Cholera Toxin-Producing Vibrio cholerae Using Loop-Mediated Isothermal Amplification
Time of Update: 2021-01-27
The LAMP assay requires 12–18 min for amplification with a single colony on selective agar from cholera toxin (CT)-producingVibrio cholerae strains and less than 60 min with human feces and seafood samples.
The latest research reminds you that youth is no use! High blood pressure accelerates cognitive decline
Time of Update: 2020-12-22
of the American Heart Association (AHA), provides us with new information. that high blood pressure at any age, no matter how long it lasts, can accelerate cognitive decline. Screenshot Source: Hypertension The study, from the Brazilian team
Using Molecular Beacons for Sensitive Fluorescence Assays of the Enzymatic Cleavage of Nucleic Acids
Time of Update: 2020-12-17
The enzymatic cleavage breaks the MB into fragments and leads to the distance separation of the quencher and the fluorophore, resulting in an increase in the fluorescent signal.
Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membrane
Time of Update: 2020-12-06
Reactive-oxygen species and organic free radicals are essential metabolic intermediates and have important regulatory functions.
Synthesis of an Esterase-Sensitive Cyclic Prodrug of a Model Hexapeptide Having Enhanced Membrane
Time of Update: 2020-12-01
The clinical development of orally active peptide drugs has been limited by their unfavorable physicochemical characteristics (e.g., charge, hydrogen bonding potential, size), which prevent them from permeating biological barriers
Experimental study on the effect of measurement system characteristics on pressure-sensitive coating calibration
Time of Update: 2020-11-28
. In order to obtain a better image quality, the light emitted by the light source should be directed vertically to the surface of the calibration film, and the camera lens plane should be parallel to the surface of the experimental part to minimize
A Sensitive Sandwich DNA Array Using Fluorescent Nanoparticle Probes
Time of Update: 2020-11-26
An ultrasensitive sandwichDNA array using highly fluorescent and photostable dye-doped silica nanoparticles is described.Compared to traditional sandwich arrays in which fluorophores have been used to signal target DNA molecules, the developednanoparticle probes provide a much stronger fluorescent emission.
The cause of low-pressure failure of small cold water machines for industrial use
Time of Update: 2020-11-18
with everyone analysis and solve the cold water machine low pressure failure. can a low pressure failure of a chiller cause? 1, increased energy consumption due to low-pressure empty operation of the compressor, will cause the motor load
Water-based cold spray zinc paint construction requirements Water-based cold spray zinc paint can be constructed using high-pressure airless spray method.
Time of Update: 2020-10-12
water-based cold spray zinc for steel structure heavy anti-corrosion coating primer, galvanized repair coating, for cold coating (spray) zinc next-generation green products.
NAT BME: Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples using a pan of thermostat.
Time of Update: 2020-10-11
Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription.
by the European Union for use in highly sensitive kidney transplant patients!
Time of Update: 2020-09-29
, the company announced that the European Commission (EC) has conditions to approve the new IgG antibody degradation enzyme Idefirix (imlifidase) for highly sensitive kidney transplant patients.
Cell Death Differ: AQP1 may be used as an indicator for predicting the sensitivity of cycloviral drugs in breast cancer patients.
Time of Update: 2020-09-23
researchers also found that miR-320a-3p can reduce the chemotherapy sensitivity of cyclopenoids by inhibiting the expression of AQP1.
Clin Neurophysiol: New sensitive electroencephalogram measurements can be used for functional connections for Alzheimer's disease.
Time of Update: 2020-08-06
, the results show that after PQ912 treatment, the functional connectivity of Early AD scants using AEC-c in the alpha band has improved.
The identification and diagnosis of gene mutations is achieved by using DNA molecular regulation and synthesis of highly sensitive gold nanoparticles.
Time of Update: 2020-08-05
In living organisms, DNA guides the synthesis of proteins. Ma Xingyi, a research professor at the Institute of Integrated Chemical Systems at Korea's Koryo University, first proposed that DNA molecules be used as skeletons to guide
BMJ: How useful are dairy products? Two servings a day can significantly reduce the risk of diabetes and high blood pressure
Time of Update: 2020-06-25
Introduction: With the improvement of living conditions, more and more people suffer from lifestyle diseases, of which metabolic syndrome is one of them. Metabolic syndrome (MetS) refers to the metabolic disorders that occur