One-Step Purification and Biochemical Characterization of Recombinant Pancreatic Lipases Expressed in Insect Cells
Time of Update: 2021-01-05
The baculovirus expression system is very convenient to produce recombinant pancreatic lipases and mutants thereof, in substantial amounts (10–50 mg of enzyme per liter of culture) for structure-function studies ( see also
Scaly-winged insects evolved in collaboration with the bee plant through their sense of smell
Time of Update: 2020-12-23
and application innovation team systematically analyzed the function of the major agricultural pest cotton bellworm odor receptor gene family, revealing a new mechanism for scaly-winged insects to evolve in concert with quilt plants through smell
Recombinant Protein Expression in the Baculovirus-Infected Insect Cell System
Time of Update: 2020-12-16
Here, we present baculovirus-infected insect cells as an efficient expression system for eukaryotic proteins.
Inhibition of Gene Expression by Nucleic Acid Enzymes in Rodent Models of Human Disease
Time of Update: 2020-12-16
Rats with postinfarction heart failure were treated with a DNA enzyme targeting TNFa, and this led to a substantial improvement of cardiac function concomitant with a restoration of the hemodynamic status of the animals.
Reconstitution of Active and Stoichiometric Multisubunit Lysine Acetyltransferase Complexes in Insect Cells
Time of Update: 2020-12-12
useful for assembling such multisubunit complexes. Related to this, we have adopted such an approach for reconstituting active tetrameric complexes ofmo nocytic leukemiaz inc (MOZ, finger protein, recently renamed MYST3 or KAT6A
Lancet Infect Dis: The epidemiological and clinical α of the emerging insect-borne virus, the emerging insect-borne virus that causes arthritis
Time of Update: 2020-12-11
arthritis-caused α viruses for clinical symptoms and signs, and laboratory confirmation is often required, especially in areas where more than one virus may be transmitted (table 2). the characteristics and nonsexual symptoms of the virus
Evaluating Effects of EPO in Rodent Behavioral Assays Related to Depression
Time of Update: 2020-12-05
Rodent behavioral models that areresponsive to clinically used antidepressants as well as to neurotrophic compounds can be used to assess potential antidepressantproperties of EPO and EPO-like compounds.
Brain Ischemic Injury in Rodents: The Protective Effect of EPO
Time of Update: 2020-12-04
Those models allow evaluating the efficiency of therapeuticstrategy of ischemia from tissular aspect to behavioral and cognitive impairment assessment.
Cloning and Expression of Human eNOS and nNOS Using the Baculovirus-Insect Cell System
Time of Update: 2020-11-26
To date, the c DNA s and genes encoding three distinct forms of the enzyme nitric oxide synthase (NOS) have been described (reviewed inref. 1 ). The family of NOS enzymes generate nitric oxide (NO) from
Intragastric Ethanol Infusion Model in Rodents
Time of Update: 2020-11-22
dissect the interfaces of these interactions for better understanding of the pathogenesis, it is essential to have an animal model that provides maximal control over ethanol and dietary intake and that enables a precise addition or deletion analysis
Insect Maintenance and Transmission
Time of Update: 2020-11-21
In order to fully understand the mechanisms of phytoplasma infection and spread, more insect vector and phytoplasma colonies will need to be established for research worldwide.
Elicitation of Jasmonate-Mediated Defense Responses by Mechanical Wounding and Insect Herbivory
Time of Update: 2020-11-13
Here, we describe a simple mechanical wounding procedure to effectively trigger JA responses in theArabidopsis thaliana rosette.
Capturing Insect Vectors of Phytoplasmas
Time of Update: 2020-11-12
While populations can be monitored by a number of passive techniques in the field, the capture of live insects is necessary for manipulation and study.
Insect Vector Transmission Assays
Time of Update: 2020-11-11
Following acquisition from the infected source plant, there is a latent period before the vector can transmit, so transmission assays consist of three basic steps: acquisition, latency, and inoculation.
Analysis of Insect-Induced Volatiles from Rice
Time of Update: 2020-11-05
VOCs. Here we describe three techniques, including solid-phase microextraction, closed-loop stripping system, and an open dynamic system, for sampling volatiles emitted from insect-damaged rice plants. In addition, the protocol for using gas chromatography-mass spectrometry to elucidate the chemical identities of rice volatiles is described.
Production, Delivery, and Use of Mycoinsecticides for Control of Insect Pests on Field Crops
Time of Update: 2020-11-05
In 1985, Pierre Ferron reviewed the status of a “hundred-year-old hypothesis” that the entomopathogemc fungi would one day become integral components of many insect-pest management systems. He concluded his review by stating the need
The content of fluorine insect pyrite does not change after the egg has been cooked.
Time of Update: 2020-09-28
Original title: Eggs after cooking fluorine content will not change since July 20, Belgium through the European Union Food Feed Rapid Warning System (RASFF) to inform the eggs detected fluorine, "poisonous eggs" incident intensified
The University of Western Ala invented the insect recipe.
Time of Update: 2020-09-27
Original title: University of Western Ala invented insect recipes crickets, flies, ants and aphids, which, although not common ingredients in Brazilian recipes, are edible, nutritious and delicious if properly farmed and cooked.
Finland pushed "the world's first insect bread" each containing 70 crickets.
Time of Update: 2020-09-17
Fazer says the bread contains more protein than normal, and that insects have fatty acids, calcium, iron and vitamin B12, which is definitely good for the body, but doesn't specify the source of the material.
The category and common species of insect pathogenic microorganisms.
Time of Update: 2020-09-13
Biomedical Channel News: There are many kinds of pathogenic microorganisms, there are fungi, bacteria, viruses, lek subsomes, protozoa and worms, among which fungi, bacteria and viruses are widely used.