Experiment 22 Exchange and absorption of ammonium ions from the root of the exosome
Time of Update: 2021-01-09
Take another triangular bottle and add 30 ml of ammonium sulfate solution, but do not put the root system for a more positive.
Analysis of F2-Isoprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization
Time of Update: 2020-12-24
Thus, it has long been recognized that there has been a need for a reliable method to assess oxidative stress status in vivo in humans.
Analysis of Glycolytic Intermediates with Ion Chromatography- and Gas Chromatography-Mass Spectrometry
Time of Update: 2020-12-20
In the next step, a silylation withN -methyl- N -trimethylsilyltrifluoroacetamide (MSTFA) is performed, giving TMS-MOX derivatives of the glycolytic intermediates.
with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-Step Lectin Affinity Chromatography
Time of Update: 2020-12-12
This protocol describes Zinc finger nuclease gene targeting of human cells to simplify the glycoproteome, as well as lectin chromatography and isolation of glycopeptides from total cell lysates of SimpleCells.
Organic Acids by Ion Chromatography
Time of Update: 2020-12-07
The presence of increased levels of various organic acids in physiological fluids such as serum, plasma, and urine has been correlated with a variety of diseases ( 1 ). Although some are rare, others such as lactic acidosis
Measurement of Isofurans by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization
Time of Update: 2020-12-04
Many methods have been developed to assess oxidative stress status in vivo, which include products of lipid, protein, and DNA oxidation.
Measurement of NOS Activity by Conversion of Radiolabeled Arginine to Citrulline Using Ion-Exchange Separation
Time of Update: 2020-11-26
This chapter describes the measurement of NOS-functional activity by determining the NOS inhibitor-sensitive conversion of radiolabeled ( 14 C or3 H) arginine to citrulline, with separation of the labeled product from substrate by ion-exchange techniques.
Metabolite Profiling of Plant Tissues by Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Time of Update: 2020-11-21
Here, we describe a detailed protocol for untargeted metabolite profiling in which methanol extracts of jasmonate-treated plant tissues are analyzed by reversed-phase liquid chromatography coupled to negative-ion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (MS).
Peptide-Based Phosphoproteomics With Immobilized Metal Ion Chromatography
Time of Update: 2020-11-14
IMAC has been useful to identify several in vivo phosphorylation sites of individual proteins, but is easier to apply to complex mixtures.
Ion exchange layering separates amino acids
Time of Update: 2020-11-02
Reagents and equipment 1, reagents and materials sodium styrene sulfonate resin (strong acid 1×8,100-200 eyes); Standard amino acid solutions: Winterline, lysine and histamine are all made into 2mg/mL of 0.1mol/L hydrochloric acid solution.
British drifter PUROLITE series ion exchange resin.
Time of Update: 2020-10-26
7, A510, large hole strong alkali styrene resin, high cross-working capacity, very high mechanical strength and permeability resistance, suitable for desalination, fluidized beds and continuous switching systems.
Ion exchange resin layering separates mixed amino acids.
Time of Update: 2020-10-26
Continue to add pH4.2 citric acid buffer to the top of the column to wash, so that the outflow pH is 4.2, close the column outlet, keep the liquid surface about 1cm above the resin surface.
Ion exchange separates amino acids.
Time of Update: 2020-10-26
(4) identification of amino acids: add 1 ml of hydrated tritone colorant to each tube collection solution and mix well, heat15 minutes in a boiling water bath, cold to room temperature to determine the light density value.
The concepts, principles and classifications of layering technology (gel and ion exchange)
Time of Update: 2020-10-20
1. Classification by physical state of the two phases gas phase layering: gas-liquid layering (fixed phase is liquid) and gas solid layering (fixed phase is solid), and flow phase is gas.
Classification of diatom mud: ion exchange
Time of Update: 2020-04-03
It is suggested to place a basin of cold water in the air conditioning room, so that the wall of diatom mud can breathe effectively.
Reject sodium cyclamate and detect the false "sweet" in liquor by Ion Chromatography
Time of Update: 2020-01-07
"No wine, no ceremony, no banquet, no wine, no custom" wine culture is a holiday atmosphere assistant for family reunion, visiting relatives and friends
Step pharmaceutical replied to the inquiry letter of Shanghai Stock Exchange late at night and disclosed the details of 8 billion sales expenses!
Time of Update: 2019-05-23
In the announcement, step pharmaceutical mainly explained the problems of high sales cost, low salary of R & D personnel, and adverse reactions of Danhong injection.
The Application of SepaFlash® Strong Anion Exchange Chromatography Columns in the Purification of Acidic Compounds
Time of Update: 2018-11-09
Separation spectrum of sample on normal phase silica gel column Figure 4.
The Application of SepaFlash® Strong Cation Exchange Chromatography Columns in the Purification of Alkaline Compounds
Time of Update: 2018-09-13
Imagine another situation: if the target product to be collected is neutral component A, the following purification strategy can be adopted: connect the SCX separation column with another positive phase silica gel column in series (see Figure 5); 3.
Stepping into a new era: new chapter of Listing Rules of biotechnology companies announced by Hong Kong Stock Exchange
Time of Update: 2018-04-25
Source: R & D customer on April 24, 2018, the Hong Kong Stock Exchange published a summary of the advisory opinions on the listing system of Companies in emerging and innovative