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Two articles by Zhejiang University scholar PNAS and others: Revealing a new mechanism for the division of cellular DNA polymerase functions
Time of Update: 2022-04-26
pressure on yeast cells by downregulating the expression level of Polε through promoter replacement , revealing for the first time a global pattern of genomic instability induced
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Nature Biotechnology: Large-scale mirror image polymerase realizes mirror DNA information storage
Time of Update: 2021-08-14
DNA information storage> With funding from the National Natural Science Foundation of China (approval numbers: 32050178, 21925702, 2175005), Zhu Ting's research group from the School of Life Sciences, Tsinghua
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Nature Communications: The mechanism by which DNA polymerase misincorporation causes genome instability
Time of Update: 2021-08-09
caused by incorrect incorporation of Pol μ is still unclear . > On June 18, 2021, the team of Professor Zhao Ye and Professor Hua Yuejin from the School of Life Sciences
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instability caused by incorrect incorporation of DNA polymerase
Time of Update: 2021-08-05
caused by incorrect incorporation of Pol μ is still unclear . > On June 18, 2021, the team of Professor Zhao Ye and Professor Hua Yuejin from the School of Life Sciences
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Real-Time Quantitative Polymerase Chain Reaction Analysis of Mitochondrial DNA Point Mutation
Time of Update: 2021-02-24
In this chapter, we describe the detailed procedures regarding the application of fluorescent probes, and real time quantitative PCR in the molecular diagnosis of mitochondrial DNA disorders.
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Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: DNA Polymerase
Time of Update: 2021-02-12
Thus, the take-home lesson in the biochemical identification of new mitochondrial activity is to develop a differential assay and to optimize the yield of the mitochondria from which extraction is pursued.
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Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction
Time of Update: 2021-01-16
The amplified products are suitable as templates for direct sequencing, or for cloning in vectors.
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Direct DNA Sequencing of Polymerase Chain Reaction Products Using Magnetic Beads
Time of Update: 2020-12-08
Furthermore, automation has been facilitated, as reaction buffers and additional reagents can be rapidly changed without centrifugation or precipitation steps.
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Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase
Time of Update: 2020-12-01
The Klenow fragment ofDNA polymerase I is a proteolytic fragment obtained by the treatment of DNA polymerase I with subtilisin. The fragment still has the polymerase and 3′–5′ exonuclease activity, but lacks the 5′–3
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E. coli DNA polymerase action and classification
Time of Update: 2020-11-03
The latter does not have the activity of 5'-rarr;3 exolytic enzymes, nor can single-stranded DNA be used as a template.
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PCR technology: Taq DNA polymerase properties
Time of Update: 2020-11-01
Based on the very low activity of salmon sperm DNA as a template, the concentration of dNTP was 0.7 to 0.8mmol/L, with different concentrations of Mg2 plus PCR reaction 10min, the result was Mgcl2 concentration at 2.2.
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E. coli DNA polymerase I
Time of Update: 2020-10-31
The latter does not have → 5' and 3 extase activity and cannot use single-stranded DNA as a template.
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DNA polymerization reactions and DNA polymerases.
Time of Update: 2020-10-26
be labeled with radioactive phosphorus (α-32PdNTP) into probes, nucleic acid molecular hybridization experiments, is an important technology of modern
molecular biology. > many experiments have confirmed that DNA polI
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Comparison of the properties of the three DNA polymerases of E. coli.
Time of Update: 2020-10-24
0cm; BORDER-BOTTOM: medium none; "valign" "top" width"101" >
single-chain molecule.
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A brief description of the function characteristics and main functions of the three DNA polymerases.
Time of Update: 2020-10-20
activity (number of nucleotides polymerized per enzyme molecule per minute at 37 degrees:D NA polymerase 1 is 600, DNA polymerase 2 is 30, and DNA polymerase 3 is 9000.
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Nat Struct and Mol Biol: Revealing how DNA polymerases ζ protect cells from DNA damage or hopefully
Time of Update: 2020-09-06
information about DNA polymerase ζ (Pol ζ), the structure and mechanism of DNA polymerase ζ that have been a mystery to scientists for years. Photo Source: CC0 Public Domain researcher Professor Anel Aggarwal says parsing the structure
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Trans-injured synthetic DNA polymerase Pol 457th sucheride can produce oxygen-to-glycosylation modification (O-GlcNAcyation)
Time of Update: 2020-08-18
not affect its ability to be recruited to the blocked replication fork and to integrate nucleotides on the opposite side of the damaged DNA template, but significantly impairs the interaction between Polium and CRL4CDT2 E3 unophine connective
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Nucleotide DNA polymerase inhibitor MIV-818 for liver cancer, awarded orphan drug title by the European Union
Time of Update: 2020-07-14
The drug has the potential to become the first liver-targeted oral drug in patients with liver cancer.
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NIH: oxidation causes human disease through DNA polymerase
Time of Update: 2014-11-27
Using a new imaging technique, researchers at the Institute of health found that the molecular mechanism for building DNA can insert molecules into DNA chains, which have been damaged by exposure to the environment.
