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DNA Sequencing at Elevated Temperature by Capillary Electrophoresis
Time of Update: 2021-01-01
Originally carried out only in poly(acrylamide) slab gels, DNA sequencing is currently to an increasing extent performed in the capillary electrophoresis (CE) format, utilizing narrow bore columns filled with a polymer sieving matrix ( 4 ).
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Replaceable Polymers for DNA Sequencing by Capillary Electrophoresis
Time of Update: 2020-12-20
In this chapter, we examine important parameters that affect the performance of replaceable polymers currently used forDNA sequencing by capillary electrophoresis (DNASCE).
Discussion of parameters affecting DNASCE resolution will be followed by comparison of experimental conditions for four linear polymers currently used in DNASCE ( 1 – 10 ).
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7 Determining Dye-Induced DNA Mobility Shifts for DNA Sequencing Fragments by Capillary Electrophoresis
Time of Update: 2020-12-02
Over the past decade, fluorescent end-labeling ofDNA fragments has evolved into the preferred method of DNA detection for a wide variety of applications, including DNA sequencing and polymerase chain reaction ( PCR ) fragment analysis.
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DNA Sequencing by Capillary Array Electrophoresis
Time of Update: 2020-12-01
On May 9,1998, PE Biosystems announced that they would introduce a new instrument based on “breakthroughDNA analysis technology” ( 1 ).
This new instrument is based on capillary array electrophoresis (CAE) with a sheath-flow cuvet detector.
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Comparative proteomics using 2-D gel electrophoresis and mass spectrometry as tools to dissect
Time of Update: 2020-11-27
We propose two-dimensional gel electrophoresis (2-DE) and mass spectrometry to define the protein components of regulons and stimulons in bacteria, including those organisms where genome sequencing is still in progress.
The basic 2-DE protocol allows high resolution and reproducibility and enables the direct comparison of hundreds or even thousands of proteins simultaneously.
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DNA Sequencing by Capillary Electrophoresis
Time of Update: 2020-11-02
DNA Sequencing by Capillary Electrophoresis is a key technology in a number experimental workflows in the life science laboratory.
The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction.
