> - methanol - water (16:9:2)>Check the color development of 10% sulfuric acid ethanol
Food Chem. Fast LC-MS/MS method for detecting 7 kinds of animal meat in meat products
Time of Update: 2021-12-01
content of some meat products may be too small for proteomics analysis . The amount of trypsin only has a slight effect on the content of peptides. 30 μL of trypsin solution (1 mg/mL
Label-Free LC-MS Method for the Identification of Biomarkers
Time of Update: 2021-03-07
Quantifying differential levels of proteins from complex biological samples like plasma or cerebrospinal fluid is one specific approach being used to identify markers of drug action, efficacy, toxicity, etc.
Analysis of Microdissected Cells by Two-Dimensional LC-MS Approaches
Time of Update: 2021-03-02
We combined the LCM with isotope-coded affinity tag (ICAT) technology and two-dimensional liquid chromatography to investigate the qualitative and quantitative proteomes of hepatocellular carcinoma (HCC).
Proteomic Analysis of Saliva: 2D Gel Electrophoresis, LC-MS/MS, and Western Blotting
Time of Update: 2021-01-31
Saliva harbors a wide spectrum of proteins that may reflect the health/disease status in the human body.
Data (Pre-)processing of Nominal and Accurate Mass LC-MS or GC-MS Data Using MetAlign
Time of Update: 2021-01-17
This paper gives a step-by-step account of how to install, set up, and run MetAlign software, which can be downloaded freely ( ).
Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS
Time of Update: 2021-01-10
Here, we describe a sensitive and specific quantification method for c-di-AMP and c-di-GMP by HPLC-coupled tandem mass spectrometry.
Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues
Time of Update: 2021-01-06
The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS).
Open-Source Platform for the Analysis of Liquid Chromatography-Mass Spectrometry (LC-MS) Data
Time of Update: 2021-01-03
The analysis of protein mixtures by liquid chromatography-mass spectrometry (LC-MS) requires tools for viewing and navigating LC-MS data, locating peptides in LC-MS data, and eliminating low-quality peptides. msInspect, an open source
Quantification of Pentose Phosphate Pathway (PPP) Metabolites by Liquid Chromatography-Mass Spectrometry (LC-MS)
Time of Update: 2020-12-29
Here we describe a simple method for comprehensive quantitative analysis of pentose phosphate pathway intermediates.
Processing and Analysis of GC/LC-MS-Based Metabolomics Data
Time of Update: 2020-12-27
Data processing forms a crucial step in metabolomics studies, impacting upon data output quality, analysis potential and subsequent biological interpretation.
Common Screening Approaches for Efficient Analytical Method Development in LC and SFC on Columns
Time of Update: 2020-12-25
. In this chapter, we will discuss simplified screening protocols and straightforward approaches to achieve chiral separations in HPLC and SFC using the column series CH IR ALPAK™ IA, IB, IC, and ID in reasonable time frame and with limited experimental work and a high success rate.
Simultaneous Analysis of Multiple Lipid Oxidation Products In Vivo by Liquid Chromatographic-Mass Spectrometry (LC-MS)
Time of Update: 2020-12-13
Quantification of F 2 -IsoPs has been regarded as the “gold standard” to assess oxidative stress status in various human diseases.
LC-MS-Based Method for the Qualitative and Quantitative Analysis of the Novel PPAR Agonist KR-62980
Time of Update: 2020-11-22
A sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for a novel peroxisome proliferator-activatedreceptor γ (PPARγ) agonist, KR-62980, in rat plasma.
Shotgun Proteomics of Plant Plasma Membrane and Microdomain Proteins Using Nano-LC-MS/MS
Time of Update: 2020-11-20
Shotgun proteomics allows the comprehensive analysis of proteins extracted from plant cells, subcellular organelles, and membranes.
The results obtained are easily applicable to label-free protein semiquantification.
Medicago truncatula Proteomics for Systems Biology: Novel Rapid Shotgun LC-MS Approach for Relative
Time of Update: 2020-11-19
In plant systems biology, several comparative studies have been carried out using liquid chromatography shotgun mass spectrometry (LC-MS/MS) and database-dependent protein identification analyses in combination with the spectral count for relative quantification.
Back to Osborne. Sequential Protein Extraction and LC-MS Analysis for the Characterization of the Holm Oak Seed Proteome
Time of Update: 2020-11-19
carried out (plant, yeast, fungi, or bacteria), the best protein extraction protocol yielded less than 20 % of the total amount of proteins, as determined by the Kjeldahl method. For this reason, protein cataloguing and the whole proteome
Making a Protein Extract from Plant Pathogenic Fungi for Gel- and LC-Based Proteomics
Time of Update: 2020-11-09
Phytopathogenic fungi, due to its particular cellular characteristics, can be considered as a recalcitrant biological material, which makes it difficult to obtain quality protein samples for proteomic analysis.
Detection and Quantification of Plant Sphingolipids by LC-MS
Time of Update: 2020-11-08
To date, the only method with the sensitivity, dynamic range, and specificity to measure all sphingolipids in a plant extract is liquid chromatography coupled to mass spectrometry.
Excellent product recommendation: Teda chemical building coatings with hydrophobic agent LC-808.
Time of Update: 2020-08-22
The original title: Excellent product recommendation: Teda chemical building coatings with hydrophobic agent LC-808 Teda chemical hydrophobic agent LC-808 for milky white low tension emulsion, added to this product can make
