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ET Recombination: DNA Engineering Using Homologous Recombination in E. coli
Time of Update: 2021-01-27
Because homologous recombination is a precise process of high fidelity, recombinogenic engineering generates DNA clones efficiently and with high precision.
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DNA Amplification: General Concepts and Methods
Time of Update: 2021-01-26
For example, the product of a PCR, often referred to as an amplification, can be readily sequenced for diagnostic, typing, fingerprinting, or molecular epidemiologic reasons.
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Isolation and Quantification of Genomic DNA from Mycobacterium tuberculosis
Time of Update: 2021-01-26
Next, add 100 ml of 5 M NaCl to the cell suspension and thoroughly mix the contents of the Eppendorf tube using a vortex.
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Why is it not uncommon for the "new crown Fuyang"? MIT Thriller Study: Viruses and DNA Combine!
Time of Update: 2021-01-26
on the preprinted platform bioRxiv, argues that the new coronavirus RNA can be reversed and integrated into the human genome. common point, the virus is hidden by combining itself with human DNA through reverse transcription. retrovirus
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Genome Plasticity: Insertion Sequence Elements, Transposons and Integrons, and DNA Rearrangement
Time of Update: 2021-01-26
This chapter discusses bacterial DNA transfer systems, transposable elements and integrons, and the contributions each makes towards the evolution of bacterial genomes, particularly in relation to bacterial pathogenesis.
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Analysis of DNA Sequences
Time of Update: 2021-01-25
Oligonucleotides can be labeled with radionucleotides or with chemiluminescent or fluorescent reporter molecules and used to probe nucleic acids extracted from samples ( 4 ), again giving qualitative or quantitative information regarding the occurrence of target organisms.
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Preparation of a Phage DNA Fragment Library for Whole Genome Shotgun Sequencing
Time of Update: 2021-01-25
Finally, examples will be given of how biological information about the phage genomic termini can be derived by analysis of theorganization of individual clones in the shotgun sequence assembly.
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Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates
Time of Update: 2021-01-24
The method involves running a first-dimension gel in order to separate restriction-digested replication intermediates of different mass.
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Bacterial DNA extraction scheme
Time of Update: 2021-01-21
CTAB/NaCl method extracts DNA with higher purity, fewer protein impurities, and long shelf life, and editors prefer to add the final concentration of 20 μg/ml RnaseA to step 5, so that there is no RnaseA contamination in the end.
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In Situ Analysis of DNA Methylation in Plants
Time of Update: 2021-01-20
Epigenetic changes in the plant genome are associated with differential genome methylation, histone modifications, and the binding of various chromatin-binding factors.
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Preparation of DNA from Arabidopsis
Time of Update: 2021-01-19
The classical approaches of plant DNA isolation are often designed to produce large amounts of DNA of high molecular weight with sufficient purity.
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Detection and Quantification of DNA Strand Breaks Using the ROPS (Random Oligonucleotide Primed Synthesis) Assay
Time of Update: 2021-01-19
The level of DSBs positively correlates with the activities of homologous and nonhomologous DNA repair pathways, which in turn inversely correlate with methylation levels and chromatin structure.
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Analysis of Bisulfite Sequencing Data from Plant DNA Using CyMATE
Time of Update: 2021-01-19
Several software tools were developed to assist in quantitative evaluation of bisulfite sequencing data from complex methylation patterns occurring in plants.
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Analysis of DNA Methylation in Plants by Bisulfite Sequencing
Time of Update: 2021-01-18
We describe the application of bisulfite sequencing for the analysis of DNA methylation at defined individual sequences of plant genomic DNA.
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T-DNA Mutagenesis in Arabidopsis
Time of Update: 2021-01-17
be used to generate large numbers of insertional mutants inArabidopsis thaliana . Using this protocol, transformation efficiencies of up to 5% can be achieved.
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INOVIO will publish data on the GBM trial of a combined treatment of DNA drugs with PD-1 inhibitors
Time of Update: 2021-01-17
On November 23, INOVIO announced that data from the DNA drug INO-5401 and INO-9012 in a new joint trial to treat newly diagnosed glioblastoma (GBM) in a joint trial with PD-1 inhibitor Libtayo® (Simipri monoma) will be published by Dr. David
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Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction
Time of Update: 2021-01-16
The amplified products are suitable as templates for direct sequencing, or for cloning in vectors.
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Isolation and Characterization of Plant Genomic DNA Sequences via (Inverse) PCR Amplification
Time of Update: 2021-01-16
The basic principle is that following heat denaturing of the DNA sample two oligodeoxynucleotide primers are allowed to anneal to their target DNA sequences located at the opposite DNA strand.
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Electroporation of DNA into the Unicellular Green Alga Chlamydomonas reinhardtii
Time of Update: 2021-01-16
Recently developed techniques for efficient transformation ( 2 – 8 ) and insertional mutagenesis ( 9 ) ofChlamydomonas nuclear and organelle genomes promise to accelerate the genetic analysis of these and other complex cellular processes.
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Development of an Efficient Inverse PCR Method for Isolating Gene Tags from T-DNA Insertional Mutants in Rice
Time of Update: 2021-01-16
The central goal of current genomics research in plants, as in other organisms, is to elucidate the functions of every gene.
