-
Primer Selection and Design for Polymerase Chain Reaction
Time of Update: 2021-03-04
Amplification of 200–400 bp DNA is the most efficient and, in these cases, one may design efficient primers simply by following a few simple rules described in this chapter.
-
Enzyme Stabilization via Bio-templated Silicification Reactions
Time of Update: 2021-03-03
A silica sol-gel process based upon biological mechanisms of inorganic material formation (biomineralization) supports protein immobilization reactions within minutes.
-
Aggregate reactions
Time of Update: 2021-03-01
For example, aggregation reaction that occurs in two or more monomers is called a co-polymerization reaction.
-
Construction of Synthetic Genes by Polymerase Chain Reaction
Time of Update: 2021-02-28
The method described in this chapter utilizes the thermostableTaq polymerase and allows for the generation of synthetic genes in as little as 1 d.
-
JACI: Study reveals molecular mechanisms for severe allergic reactions
Time of Update: 2021-02-28
lead to death. reactions can be caused by a variety of causes, such as allergic reactions to food, drugs, or insect venom. molecular mechanisms that cause such reactions to be severe are still unknown. study, led by researchers
-
Multiplex Polymerase Chain Reaction
Time of Update: 2021-02-27
When these assays must be performed repetitively on multiple samples, the multiplex PCR, which allows for simultaneous amplification of 2–50 different targets ( 13 – 15 ), is potentially very useful from a perspective of time, and money.
-
BMJ: Milk is the most common cause of fatal allergic reactions in children in the UK
Time of Update: 2021-02-26
, there was an increase in hospital admissions for food-related allergic reactions in the UK, but a decrease in the number of deaths. in school-age children, milk allergies are the single most common cause of fatal allergic reactions.
-
Real-Time Quantitative Polymerase Chain Reaction Analysis of Mitochondrial DNA Point Mutation
Time of Update: 2021-02-24
In this chapter, we describe the detailed procedures regarding the application of fluorescent probes, and real time quantitative PCR in the molecular diagnosis of mitochondrial DNA disorders.
-
Cell: Innovative nanoparticle candidate new crown vaccines trigger super-effective meso-antibody reactions
Time of Update: 2021-02-24
The production schematic shows how coronavirus proteins were added to a computer-designed nanoparticle platform to create a candidate vaccine for COVID-19.
-
Six-flavored yellow pill modification instruction manual Adverse reactions increased by 13 items
Time of Update: 2021-02-24
Pharma Network February 20, recently, the State Drug Administration issued a notice on the revision of the six-flavored yellow preparation instructions, which added 13 adverse reactions. The State Drug Administration reminds the relevant drug
-
Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism Monitoring of Genes
Time of Update: 2021-02-24
Considerable effort has led to the development and application of a number of molecular procedures to profile the diversity of microbial sequences in environments.
-
Allergy: New immunotherapy can treat cat allergic reactions
Time of Update: 2021-02-23
"We're trying to explore new ways to increase AIT anti-inflammatory activity with CpG, a known immunomodulator, but at a higher safe dose than previously used for this type of therapy," explains allergy and immunologist Dr. Cathy Léonard.
-
Guizhou insurance compensation vaccine abnormal reaction
Time of Update: 2021-02-22
Through commercial insurance, the province will provide basic insurance services for 1.6 million vaccinated children for the three years from 1 January 2018 to 31 December 2020.
-
From Metabolic Reactions to Networks and Pathways
Time of Update: 2021-02-21
This chapter introduces well-known topological transformations from metabolic reactions to a graph, and discusses their advantages and disadvantages.
-
Rapid Detection and Identification of Dengue Viruses by Reverse Transcriptase/Polymerase Chain Reaction
Time of Update: 2021-02-18
Introduction of the polymerase chain reaction ( PCR ) technique as a rapid and sensitive method for amplification ofDNA has resulted in development of new specific nucleic acid-based techniques for clinical diagnosis of infections ( 1 ).
-
Quick and reliable method to analyze meiotic segregation patterns in Coprinus cinereus using the polymerase chain reaction
Time of Update: 2021-02-18
to permit the destruction of contaminating amplified DNAs. If contamination is detected in a negative control, uracil N-glycosylase can be used since this enzyme cleaves sequences which contain dUTP. The advantages of our method include: 1
-
Guangdong vaccination abnormal reaction compensation is included in the commercial insurance
Time of Update: 2021-02-17
or the second type of vaccine purchased under basic insurance will be compensated by commercial insurance. In addition, parents can voluntarily purchase supplemental insurance for their babies at their own expense. is the most direct, economical
-
Analysis of Human Cytomegalovirus Using the Polymerase Chain Reaction
Time of Update: 2021-02-17
Similarly, reverse transcription-PCR ( RT-PCR ) allows the study of targeted gene expression, by reverse transcription of RNA to complementary DNA ( cDNA ), followed by amplification of target DNA using predetermined primers.
-
Virus Genome Detection by the Polymerase Chain Reaction (PCR)
Time of Update: 2021-02-16
Since the polymerase chain reaction ( PCR ) technique was developed in 1986 ( 1 ), it has found wide application in molecular biology and is now regarded as an irreplaceable tool in many laboratories. This chapter will deal with its use for the detection of virus genomes for diagnostic and experimental purposes.
-
Detection of Escherichia coli O157:H7 by Immunomagnetic Separation and Multiplex Polymerase Chain Reaction
Time of Update: 2021-02-15
coli (VTEC) have been identified as the cause of increasing numbers of cases of serious human illness ( 1 , 2 ).
