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Linkers, Resins, and General Procedures for Solid-Phase Peptide Synthesis
Time of Update: 2021-03-07
The chapter finishes with robust protocols for general solid-phase peptide synthesis, i.e., the standard operations.
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An Approach Combining Rapid cDNA Amplification and Chemical Synthesis for the Identification of Novel, Cathelicidin-Derived, Antimicrobial Peptides
Time of Update: 2021-02-21
Host defense in mammals involves a cooperative effort by a variety of antimicrobial peptides ( 1 ), which are produced by both phagocyte precursors and some epithelia. They are called on to combat invading pathogens at sites
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What are the advantages and disadvantages of NEB's M13 phage random peptide library and NOVAGE's T7 phage peptide library?
Time of Update: 2021-01-24
s good, everyone! I have a few questions about PHAGE DISPLAY that I would like to ask you. Could you tell me about the advantages and disadvantages of Ph.D and and M13 c dna and peptide library? Under what circumstances
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Rapid Liquid-Phase Combinatorial Synthesis of Heterocyclic Libraries
Time of Update: 2021-01-03
In the past two years, both academic institutes and pharmaceutical companies have developed novel combinatorial techniques for library synthesis equally.
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PGVL Hub: An Integrated Desktop Tool for Medicinal Chemists to Streamline Design and Synthesis of Chemical Libraries and Singleton Compounds
Time of Update: 2021-01-03
PGVL Hub is an integrated molecular design desktop tool that has been developed and globally deployed throughout Pfizer discovery research units to streamline the design and synthesis of combinatorial libraries and singleton compounds.
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Tea Bag Synthesis of Positional Scanning Synthetic Combinatorial Libraries and Their Use for Mapping Antigenic Determinants
Time of Update: 2020-12-20
Positional scanning synthetic combinatorial libraries (PS-SCLs) are useful in identifying the amino acid sequences of antigenic determinants recognized by monoclonal antibodies (MAbs).
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Synthesis of Lipidated Peptides
Time of Update: 2020-12-08
Despite their essential role on protein function, the synthesis of fully posttranslationally modified proteins has been challenging.
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Synthesis of Oligonucleotide-Peptide and Oligonucleotide-Protein Conjugates
Time of Update: 2020-12-05
These hybrid molecules can be used deliver chemical functionality to specific sequences within deoxyribonucleic acid and ribonucleic acid.
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Production of Double-Stranded cDNA for Gene Library Synthesis
Time of Update: 2020-12-05
The quality and length of the cDNA product largely depends on the quality of the mRNA used as the starting material.
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Synthesis of Coumarin-Based, Esterase-Sensitive Cyclic Prodrugs of Opioid Peptides with Enhanced Membrane Permeability and Enzymatic Stability
Time of Update: 2020-12-02
In this chapter, we wish to describe a third method of making esterase-sensitive cyclic prodrugs of peptides using DADLE, an opioid peptide ( 4 – 7 ), as an example.
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SNAr-Based Cycloetherification Methodology: Application in the Synthesis of Heterodectic
Time of Update: 2020-12-01
The endo aryl-aryl and aryl-alkyl ether bonds exist in a number of biologically important macrocyclic natural products, such as vancomycin family glycopeptide antibiotics (1 –3 ), antitumor series RA I-XIV (4
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Mimotopes of tumor-associated T-cell epitopes for cancer vaccines determined with combinatorial peptide libraries
Time of Update: 2020-11-28
The identification of tumor-associated epitopes for these cells, therefore, has become a key aspect of the development of cancer vaccines.
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Synthesis, Characterization, and Evaluation of Radiometal-Containing Peptide Nucleic Acids
Time of Update: 2020-11-28
Peptide nucleic acids (PNAs) have very attractive properties for applications in nuclear medicine.
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Microwave-Assisted Solid-Phase Peptide Synthesis Using the Biotage Syro Wave
Time of Update: 2020-11-25
Fast and precise heating by microwave irradiation during solid-phase peptide synthesis (SPPS) can reduce reaction times as well as provide better purities and greater yields for the synthesis of difficult peptides.
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Epitope Mapping Using Multipin Peptide Synthesis
Time of Update: 2020-11-25
Which amino acids in the epitope are in contact with the antibody/TCR/MHC molecule?
What variants of the epitope are still recognized by the antibody/TCR/MHC molecule?
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Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library
Time of Update: 2020-11-23
We have identified a peptide substrate for molecular chaperone Hsp40 Ydj1 by utilizing the combination of phage display library screening and isothemol titration calirimetry (ITC).
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The principle of peptide synthesis
Time of Update: 2020-11-01
in order to avoid side reactions, we need to protect unreactable aminos in advance so that they do not participate in the synthesis process, thus avoiding the generation of by-products.
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Synthesis of solid-phase peptides.
Time of Update: 2020-10-29
a small amount of flocs insoluble, resulting in the addition of too many solvents to form an unsaturated solution, so that the crystal yield is low or non-crystallization. For this purpose, the crystalline solution needs filtering to remove floc
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Common questions and answers for peptide synthesis.
Time of Update: 2020-10-29
These methods of insoluble peptides require DMF (dimethyl methamide), niacin, guanidinium (salt), chloride (chloride), or acetonitrile (acetylene) to dissolve, and these solvents may have side effects in some experiments.
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The beginning of peptide chain synthesis.
Time of Update: 2020-10-29
any codon (N for any nucleotie). Note.The ribosome also has an exit site not show in the diagram. This site will be discussed later. the effect of eIF-5, it is able to combine Met-tRNAfmet, GTP and mrNAR40S sub-sub-base with 60S large
