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Detection and Quantification of DNA Strand Breaks Using the ROPS (Random Oligonucleotide Primed Synthesis) Assay
Time of Update: 2021-01-19
The level of DSBs positively correlates with the activities of homologous and nonhomologous DNA repair pathways, which in turn inversely correlate with methylation levels and chromatin structure.
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Detection of Changes in Global Genome Methylation Using the Cytosine-Extension Assay
Time of Update: 2021-01-18
Although there are various methods of methylation analysis, most of them are either laborious or expensive, or both.
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Pull-Down Assays for Plant Hormone Research
Time of Update: 2021-01-17
Hormonal signals are transduced (and sometimes perceived) by protein–protein interactions.
Understanding these interactions is therefore crucial to understanding the network of signalling components as a whole.
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Chloramphenicol Acetyl Transferase Assay
Time of Update: 2021-01-16
The chloramphenicol acetyltransferase(cat) gene, isolated from transposon Tn9 ofEscherichia coli ( 1 ), is a convenient genetic marker for studies of transformation.
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Low VOC metal finish with concentrated aluminum strips
Time of Update: 2021-01-16
This paper focuses on Benda-Lutz®COMPAL WS, a new type of non-critical concentrated aluminum strip specifically designed for water-based coatings and inks with long-term stability.
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Analysis of Locus-Specific Changes in Methylation Patterns Using a COBRA (Combined Bisulfite Restriction Analysis) Assay
Time of Update: 2021-01-15
Methylation analysis at a given locus allows one to evaluate levels of chromatin packaging, gene expression, and even homologous recombination.
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Enzyme-Linked Immunosorbent Assay for the Detection and Identification of Plant Pathogenic Bacteria
Time of Update: 2021-01-15
Enzyme-linked immunosorbent assay (ELISA) is the most commonly used serological diagnostic technique. A number of different ELISA formats can be used for the detection of bacterial plant pathogens and in particularErwinia amylovora andClavibacter michiganensis subsp. sepedonicus .
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Enzyme-Linked Immunosorbent Assay Detection of Ralstonia solanacearum in Potatoes: The South African Experience
Time of Update: 2021-01-14
In this chapter, the development of an enzyme-linked immunosorbent assay (ELISA) for the detection ofRalstonia solanacearum including antibody production, the ELISA method itself, potato sample preparation for testing, ELISA result validation and interpretation, and confirmation of infection are discussed.
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Analysis of Plant Regulatory DNA Sequences by Transient Protoplast Assays and Computer Aided Sequence Evaluation
Time of Update: 2021-01-14
The orchestrated regulation of hundreds of genes responding in a temporal, spatial, and conditional expression is in part mediated by the transient binding of transcription factors to their specific DNA motifs.
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Reporter Genes and Transient Assays for Plants
Time of Update: 2021-01-14
A series of DNA plasmids containing reporter genes encoding readily assayed enzymes are available for this purpose.
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Transient Assays for the Analysis of miRNA Processing and Function
Time of Update: 2021-01-13
For small RNA analysis in plants, the most widely used method, commonly named agroinfiltration, makes use ofAgrobacterium tumefaciens to deliver transgenes into leaf cells ofNicotiana benthamiana .
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Primer Extension Assay
Time of Update: 2021-01-10
. Primer extension assays have been used mainly in mapping of 5′ termini of RNA transcripts, but have also been of value, in, for example, analysis of nuclear pre-mRNA splicing ( 1 – 3 ), and in the detection of low abundance RNA
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Phosphoinositidase C Activation Assay III: HPLC Analysis of Cellular Phosphoinositides and Phosphoinositols
Time of Update: 2021-01-06
However, in reality, the metabolism of the phosphoinositols is complex ( 1 ) leading to the formation of several isomeric forms in each class.
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Chimeric Templates and Assays Used to Study Physarum Cotranscriptional Insertional Editing In Vitro
Time of Update: 2021-01-06
This chapter describes in vitro transcription systems in which mtTEC RNAs are elongated on repositioned parts of the genome or exogenous DNA, in order to investigate how the nontemplated insertions are directed.
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Assay of Spermidine and Spermine Synthases
Time of Update: 2021-01-03
C. 2.5.1.16), which is present in virtually all organisms, transfers the aminopropyl group from dcAdoMet to putrescine forming spermidine.
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Assay of Phospholipid Hydroperoxides by Chemiluminescence-Based High-Performance Liquid Chromatography
Time of Update: 2021-01-03
. One of the most advanced methods for the detection of LOOH is a chemiluminescence-based high-performance liquid chromatography (HPLC) assay ( 1 – 5 ) as shown in Fig. 1 . The reaction sequence leading to the emission
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Quantitative and Robust Assay to Measure CellCell Contact Assembly and Maintenance
Time of Update: 2021-01-02
However, we find that this method has limitations on the quantification (dynamic range), ability to detect partial phenotypes and to differentiate between the participation of a given regulatory protein in assembly and/or maintenance of cell–cell contacts.
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Studying Kinesins Enzymatic Cycle Using a Single-Motor Confocal Motility Assay, Employing Frster Resonance Energy Transfer
Time of Update: 2021-01-01
It is the smallest motor protein known that converts free energy obtained from ATP hydrolysis into mechanical work, by stepping along microtubules.
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The Burton Assay for DNA
Time of Update: 2021-01-01
The Burton assay forDNA is a colorimetric procedure for measuring the deoxyribose moiety of DNA.
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Characterization of ProteinMembrane Binding Interactions via a Microplate Assay Employing Whole Liposome Immobilization
Time of Update: 2020-12-30
Herein, we describe a microplate-based method for the detection of protein–membrane binding that employs whole liposome immobilization using a biotin anchor.
