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Research reveals new functions of Golgi membrane protein PAQR11 on lipid metabolism and immune regulation
Time of Update: 2021-03-23
by PAQR11 via altering protein stability of phosphodiesterase 4D by Chen Yan’s research group. Protein stability affects lipolysis, thereby regulating the body's obesity symptoms.
Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex Subunit Proteins From Higher Plants
Time of Update: 2021-03-13
The OEC subunit proteins are dissociated from the PSII-enriched membranes by alkaline treatment or salt treatment, and then purified by ion-exchange chromatography using an automated high-performance liquid chromatography system.
One- and Two-Dimensional Blue Native-PAGE and Immunodetection of Low-Abundance Chloroplast Membrane Protein Complexes
Time of Update: 2021-03-12
Blue native polyacrylamide gel electrophoresis (BN-PAGE) is a powerful method for separating protein complexes from biological membranes under native conditions.
Plasma protein and clotting
Time of Update: 2021-03-06
physiological conditions, blood flows through blood vessels throughout the body, transporting substances between tissues.
Laurdan Studies of Membrane Lipid-Nicotinic Acetylcholine Receptor Protein Interactions
Time of Update: 2021-03-04
The extrinsic fluorescent probe Laurdan (6-dodecanoyl-2-dimethylamino naphthalene) exhibits extreme sensitivity to the polarity and to the molecular dynamics of the dipoles in its environment. Dipolar relaxation processes are reflected
Electron Crystallography of Membrane Proteins
Time of Update: 2021-03-02
However, sample preparations that sandwich the two-dimensional crystals between symmetrical carbon films reduce the charge-induced specimen drift, and the flatness of the preparations can be optimized by the choice of the grid material and the preparation protocol.
Membrane Insertion of Small Proteins
Time of Update: 2021-02-27
The insertion of the protein into proteoliposomes can be followed by protease digestion and Western blot analysis.
Plasma Nutrition introduces low-calorie protein supplements
Time of Update: 2021-02-20
before the product was officially launched, the University of South Florida found that 20g IoProtein - Reduced Calorie achieved the same effect as 27g standard whey separation protein.
Analysis of Bacterial Outer Membrane Proteins
Time of Update: 2021-02-16
The cell envelope of Gram-negative bacteria comprises an inner and an outer membrane separated by a layer of peptidoglycan (Fig. 1 ).
Use of a Yeast-Based Membrane Protein Expression Technology to Overexpress Drug Resistance Efflux Pumps
Time of Update: 2021-02-14
We have developed a heterologous membrane protein expression system that can be used to study the structure and function of these proteins in the non-pathogenic, genetically stable, and versatile eukaryotic model organism,Saccharomyces cerevisiae .
Neurology: Inflammation-related plasma proteins can reflect seizures
Time of Update: 2021-02-02
Accurate diagnosis of epilepsy is challenging because clinicians rarely observe actual clinical seizures outside the hospital. addition, psychotic non-epileptic seizures (PNES) can mimic seizures (ES), leading to incorrect diagnosis
Assembly of Bacterial Outer Membrane Proteins
Time of Update: 2021-02-01
Various methods that are routinely used to study the subcellular localization of membrane proteins in wild-type Gram-negative bacteria fall short in genetic studies addressing the biogenesis of outer membrane proteins (OMPs).
Preparation of Blotted Membrane for Protein Microsequencing
Time of Update: 2021-01-31
This chapter aims to provide a step-by-step procedure in preparing protein samples of limited quantities for partial sequence analysis.
Analysis of Interactions Between Viral Replicase Proteins and Plant Intracellular Membranes
Time of Update: 2021-01-19
Because many plant viruses replicate in association with the endoplasmic reticulum (ER), we also discuss two techniques to specifically analyze the interaction of viral proteins with these membranes.
Membrane and Protein Dynamics in Virus-Infected Plant Cells
Time of Update: 2021-01-16
Therefore, to meet the challenges of diverse cell biology studies that will be derived from ongoing functional genomics projects, a series of methods relevant to the characterization of membrane and protein dynamics in virus-infected cells are provided here.
Nuclear Magnetic Resonance Structural Studies of Membrane Proteins in Micelles and Bilayers
Time of Update: 2021-01-05
Nuclear magnetic resonance ( NMR ) spectroscopy enables determination of membrane protein structures in lipid environments, such as micelles and bilayers.
Purification and Proteomic Analysis of Lysosomal Integral Membrane Proteins
Time of Update: 2021-01-05
Here, we describe two comparable and easy methods to isolate lysosomes from mouse or rat liver in sufficient quantities for proteomics studies.
Surface Plasmon Resonance Spectroscopy in Determination of the Interactions Between Amyloid Proteins (A) and Lipid Membranes
Time of Update: 2021-01-04
Surface plasmon resonance (SPR) spectroscopy is emerging as a useful tool for determination of molecular interactions in real time.
Crystallization and Structure Analysis of Membrane Proteins
Time of Update: 2021-01-04
The structure of another, entirely different membrane protein, the bacterial outer membrane porin fromRhodobacter capsulatus , has now been determined by X-ray crystallography ( 6 ).