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    dideoxy dna sequencing

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    • DNA Sequence Determination Using Dideoxy Analogs

      Time of Update: 2020-12-05
      In the presence of the four nucleoside triphosphates (dNTPs), one of which is radioactively labeled, and the Klenow fragment of DNA polymerase, primed synthesis occurs across the region of interest.
    • Direct Dideoxy DNA Sequencing

      Time of Update: 2020-11-30
      The procedure requires a plasmid DNA template that is denatured to separate the DNA strands and a primer complementary to one of the strands of the plasmid vector adjacent to the cloning site that has been used.

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