Determination of the molecular weight of DNA-binding proteins using UV-crosslinking and SDS-PAGE
Time of Update: 2021-03-01
Following irradiation with a UV light source, the DNA/protein complex is subjected to SDS-PAGE and its molecular weight determined by comparison with appropriate protein standards.
An SDS-PAGE-Based Enzyme Activity Assay for the Detection and Identification of Histone Acetyltransferases
Time of Update: 2021-02-15
Posttranslational acetylation of the core histone amino-terminal tails correlates with both chromatin assembly and gene expression. This energy-intensive and reversible process is mediated by the opposing activities of histone
Purification of Bacteriophages and SDS-PAGE Analysis of Phage Structural Proteins from Ghost Particles
Time of Update: 2021-02-13
These particles should be preferred to the entire bacteriophages for one-dimensionalSDS -PAGE analysis of phage structural proteins, since running of the phage proteins through the gel is not disturbed by the presence of the phage DNA.
Ceruloplasmin Detection by SDS-PAGE, Immunoblotting, and In Situ Oxidase Activity
Time of Update: 2020-12-05
Ceruloplasmin is an acute-phase reactant that functions as a ferroxidase, oxidizing Fe ++ to Fe +++ , the latter being able to combine with apotransferrin to form transferrin, the primary iron transport protein in the plasma.
Horizontal SDS-PAGE for IPG-Dalt
Time of Update: 2020-11-30
For vertical setups, the equilibrated IPG gel strips are loaded on top of vertical SDS polyacrylamide gels and embedded in agarose ( see Chapter 26 ).
SDS-PAGE-Based Quantitative Assay for Screening of Kidney Stone Disease
Time of Update: 2020-11-26
We developed aSDS -PAGE-based method to quantify Tamm Horsfall glycoprotein (THP) for screening of kidney stone disease.
Comparative Analysis of Cytoplasmic Membrane Proteomes of Escherichia coli Using 2D Blue Native/SDS-PAGE
Time of Update: 2020-11-23
Immobilization prevents distortion of BN-gel lanes when they are transferred to the 2nd dimension, which lowers variation and greatly improves reproducibility of 2D BN/SDS-gels.
SDS-PAGE Separation of Proteins Produced by Beans During Development
Time of Update: 2020-11-22
/> Proteins produced during the process of seed germination in beans will be separated using SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE in this laboratory). Beans will be allowed to germinate for different periods of time ranging from zero to ten
Analysis of Rice Proteins Using SDS-PAGE Shotgun Proteomics
Time of Update: 2020-11-19
In this chapter we describe the workflow used in our laboratory to analyze rice leaf samples using label-free shotgun proteomics based onSDS -PAGE fractionation of proteins.
SDS-Polyacrylamide Gel Electrophoresis (PAGE)
Time of Update: 2020-10-27
, experimental purpose: 1. Learn the principle of SDS -PAGE separation protein ; II, experimental principles: 1, electrophoresis: (1) definition: refers to the charged particles
SDS-PAGE, protein transfer, western Blot reagent preparation.
Time of Update: 2020-10-20
(see kara company Catalog-N1) 1 mol/L Tris (PH6.8) 1.
Preparation and operation of SDS-PAGE experimental reagent
Time of Update: 2014-08-01
3. Ph8.9 separation gel buffer: tris 36.3g, add 1mol / L HCl 48ml, add 80ml of steaming water to make it dissolve, adjust ph8.9, fix the volume to 100ml, and store at 4 ℃.
SDS-PAGE problems and common problems analysis
Time of Update: 2014-05-15
What's the principle of lamination (or concentrate)? Weak acids in buffer system, such as glycine, seldom dissociate at ph6.7, and their effective swimming rate is very low, while