Cooperation | Jingtai Technology joins hands with Sigma Biosciences to promote major progress in the diffuse gastric cancer project
Time of Update: 2021-04-18
of "AI algorithm + expert experience + drug experiment" to formulate a full set of artificial intelligence drug discovery solutions for the project, including early-stage target information collection and screening, AI model construction and evaluation
Determination of the molecular weight of DNA-binding proteins using UV-crosslinking and SDS-PAGE
Time of Update: 2021-03-01
Following irradiation with a UV light source, the DNA/protein complex is subjected to SDS-PAGE and its molecular weight determined by comparison with appropriate protein standards.
Spectrum has released its latest beverage custom ingredient: Sigma-Beverage
Time of Update: 2021-02-17
However, by choosing a combination of stevia glycosides with different sweet tastes that result in pure, sugar-like flavors and reduced sweetness stay time, we were able to create a stevia glycoside ingredient to effectively accept the challenges of beverage applications.
An SDS-PAGE-Based Enzyme Activity Assay for the Detection and Identification of Histone Acetyltransferases
Time of Update: 2021-02-15
Posttranslational acetylation of the core histone amino-terminal tails correlates with both chromatin assembly and gene expression. This energy-intensive and reversible process is mediated by the opposing activities of histone
Purification of Bacteriophages and SDS-PAGE Analysis of Phage Structural Proteins from Ghost Particles
Time of Update: 2021-02-13
These particles should be preferred to the entire bacteriophages for one-dimensionalSDS -PAGE analysis of phage structural proteins, since running of the phage proteins through the gel is not disturbed by the presence of the phage DNA.
Professor Chen Jie, Jiangnan University: Changes in the metabolism of SD rats caused by harmful Merad reaction products and high-fat diets
Time of Update: 2021-01-29
National Key Laboratory for Food Science and Technology feed rats with high-fat feed and use acrylamide (21 mg/kg BW), halmantine (7 mg/kg BW), N Ɛ -(carboxymethyl) lysine (carboxymethyl), CML; 10mg/kg BW) treatment or the joint
Ceruloplasmin Detection by SDS-PAGE, Immunoblotting, and In Situ Oxidase Activity
Time of Update: 2020-12-05
Ceruloplasmin is an acute-phase reactant that functions as a ferroxidase, oxidizing Fe ++ to Fe +++ , the latter being able to combine with apotransferrin to form transferrin, the primary iron transport protein in the plasma.
Horizontal SDS-PAGE for IPG-Dalt
Time of Update: 2020-11-30
For vertical setups, the equilibrated IPG gel strips are loaded on top of vertical SDS polyacrylamide gels and embedded in agarose ( see Chapter 26 ).
SDS-PAGE-Based Quantitative Assay for Screening of Kidney Stone Disease
Time of Update: 2020-11-26
We developed aSDS -PAGE-based method to quantify Tamm Horsfall glycoprotein (THP) for screening of kidney stone disease.
Comparative Analysis of Cytoplasmic Membrane Proteomes of Escherichia coli Using 2D Blue Native/SDS-PAGE
Time of Update: 2020-11-23
Immobilization prevents distortion of BN-gel lanes when they are transferred to the 2nd dimension, which lowers variation and greatly improves reproducibility of 2D BN/SDS-gels.
SDS-PAGE Separation of Proteins Produced by Beans During Development
Time of Update: 2020-11-22
/> Proteins produced during the process of seed germination in beans will be separated using SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE in this laboratory). Beans will be allowed to germinate for different periods of time ranging from zero to ten
Analysis of Rice Proteins Using SDS-PAGE Shotgun Proteomics
Time of Update: 2020-11-19
In this chapter we describe the workflow used in our laboratory to analyze rice leaf samples using label-free shotgun proteomics based onSDS -PAGE fractionation of proteins.
Determination of protein molecular weight (SDS-polyacrylamide gel electrophoresis)
Time of Update: 2020-10-30
of various proteins. ethanol is a reducing agent of the two sulfur bonds in protein molecules, so that peptides components are divided into a single sub-unit. SDS breaks the hydrogen bond of a protein. Therefore, when it binds to proteins
The SDS-polyacrylamide gel electrophoresis method measures the relative molecular weight of proteins.
Time of Update: 2020-10-29
18.15 Tris (trihydroxy methamethane), plus about 80 ml of heavy steamed water, with 1mol/LHCl to pH to 8.8, diluted with heavy steamed water to the final volume of 100 ml, 4 degrees C refrigerator storage.
SDS-Polyacrylamide Gel Electrophoresis (PAGE)
Time of Update: 2020-10-27
, experimental purpose: 1. Learn the principle of SDS -PAGE separation protein ; II, experimental principles: 1, electrophoresis: (1) definition: refers to the charged particles
Yeast immunofluorescent staining -- Immunofluorescent Staining of Yeast: SDS Permeabilization Metho.
Time of Update: 2020-10-26
After diluting in PBT buffer spinsecondary in microfuge (at 4°C) for 5 min to pellet any debris. 14) SecondaryAntibodies: Jackson Immunoresearch Laboratoriesmakes high quality secondary antibodies which are good forboth single and double labeling experiments.
SDS-PAGE, protein transfer, western Blot reagent preparation.
Time of Update: 2020-10-20
(see kara company Catalog-N1) 1 mol/L Tris (PH6.8) 1.
Blood: Prognosis for hematopoietic cell transplantation treatment for Wiskott-Aldrich syndrome
Time of Update: 2020-06-24
A high proportion of donor myelin cell transplantation is essential for platelet reconstruction.
The biggest chemical purchase in history has come to an end perfectly! Merck's $17 billion acquisition of sigma Aldrich
Time of Update: 2015-11-20
Source: Biovalley November 20, 2015 Merck KGaA, a German pharmaceutical and chemical giant, recently announced that it has successfully completed the US $17 billion cash
Honeywell's € 105 million acquisition of sigma Aldrich research chemicals
Time of Update: 2015-10-23
Source: Biovalley 2015-10-23 October 23, 2015, Honeywell announced on the 20th that it will acquire sigma Aldrich research chemicals business for 105 million euros, and obtain