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Detecting mRNAin Tissue Sections with Digoxigenin-Labeled Probes
Time of Update: 2021-03-06
, digoxigenin can be used to label either RNA,DNA , or oligonucleotide probes. There have been different opinions on the sensitivity of detection of digoxigenin probes, but recent publications have shown that the sensitivity of radiolabeled and nonradioactive probes is comparable ( 3 ).
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Fluorescent Sample Labeling for DNA Microarray Analyses
Time of Update: 2021-03-06
The third method is based on an amplification of antisense RNA by in vitro transcription subsequent to first- and second-strand complementary DNA synthesis.
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On-Column Labeling Reaction for Analysis of Protein Contents of a Single Cell Using Capillary Electrophoresis With Laser-Induced Fluorescence Detection
Time of Update: 2021-03-05
A custom-made CE instrument with laser-induced fluorescence (LIF) detection, used for the analyses, is described.
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Improving image analysis in 2DGE-based redox proteomics by labeling protein carbonyl with fluorescent hydroxylamine
Time of Update: 2021-03-03
In this study, we present a new method which allows detection of protein oxidation and total protein on the same gel to improve matching in image analysis.
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Sequence-Specific DNA Labeling Using Methyltransferases
Time of Update: 2021-03-03
DNA methyltransferases (MTases) naturally possess these two functions and transfer a methyl group from the cofactorS -adenosyl- L -methionine (AdoMet) to adenine or cytosine residues within specific DNA sequences, typically ranging from two to eight base pairs.
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End-Labeling of DNA Fragments
Time of Update: 2021-03-03
If double-stranded DNA is used, both ends are labeled, but single-end labeled fragments can be produced by further restriction enzyme digestion.
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Takeda HyQiva has been approved by the European Union to update the label
Time of Update: 2021-03-02
Takeda recently announced that the European Commission (EC) has approved a label update for HyQvia (10%), recombinant human hyalurase, expanding its use to make it the first and only easy-to-use subsurface immunoglobulin replacement therapy
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Hybridization of Horseradish Peroxidase-Labeled Probes and Detection by Enhanced Chemiluminescence
Time of Update: 2021-03-02
This is shown by the diversity of applications in which this labeling and detection system has been used.
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Analyzing folding and degradation of metabolically labelled polypeptides by conventional
Time of Update: 2021-03-02
Efficient protein folding and quality control are essential for unperturbed cell viability.
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Preparation of Direct, Enzyme-Labeled DNA Probes
Time of Update: 2021-03-02
The methodology was combined with enhanced chemiluminescence ( 2 ) (a light-producing HRP catalyzed reaction based on luminol) ( see Chapter 26 ) allowing the detection of specific hybrids on membranes ( 3 ).
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Arachidonic Acid Labeling
Time of Update: 2021-03-01
6) Remove label by washing cells 3 X with ice cold media.
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Enzyme-Labeled DNA Probes
Time of Update: 2021-03-01
The presence of certain types of human papillomavirus in cervical smears can be used as an indicationof neoplasia ( 2 ).
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Fluorescent Labeling of Proteins
Time of Update: 2021-02-27
Many single-molecule experimental techniques exploit fluorescence as a tool to investigate conformational dynamics and molecular interactions or track the movement of proteins in order to gain insight into their biological functions.
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The EU has expanded the label for the use of GSK/Innoviva's slow-stop pulmonary inhalers
Time of Update: 2021-02-26
The European Commission has expanded glaxoSmithKline/Innoviva's once-a-day Trelegy Ellipta label to make it the first single-inhaled triple therapy for patients with moderate to severe slow-blocking lungs (LCPs), who are treated with corticosteroids
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Microwave Polymerization in Thin Layers of London Resin White Allows Selection of Specimens for Immunogold Labeling
Time of Update: 2021-02-26
The most time-consuming step in immunogold labeling does not begin until the sections are viewed inside the transmission electron microscope (TEM), specifically, locating regions that have been labeled with gold particles.
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One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and Identification of 3H-Labeled RNA by Fluorography
Time of Update: 2021-02-26
Possible applications include examining products of in vitro synthesis, hybrid-selected RNAs from total cellular nucleic acids, and Northern blots.
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Biosynthetic labeling
Time of Update: 2021-02-25
If, however, you want to look at the steady-state abundance of phosphate in proteins, lipids, or RNA, an over-night labeling period may be the best.
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Hybridization of Digoxigenin-Labeled Probes to Southern Blots and Detection by Chemiluminescence
Time of Update: 2021-02-25
Probes prepared with either digoxigenin- or biotin-modified nucleotides can be hybridized to Southern blots to detect target nucleic acid sequences.
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Phosphoinositidase C Activation Assay I: Cell Labeling, Stimulation, and Recovery of Cellular [3H]Phosphoinositides and [3H]Phosphoinositols
Time of Update: 2021-02-24
For phosphoinositols, abbreviations are in the form Ins(x,y,z )Pn , wherex, y , andz refer to the positions of the phosphate groups on the myo-inositol ring andn refers to the total number of phosphates.
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BASF has added Onecide P EC (Serifluoro herbicide) to its portfolio of soy herbicides
Time of Update: 2021-02-24
OnecideP is a selective leaf herbicide containing the active ingredient pyridoxine, which effectively controls one-year and perennate weeds.
