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Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant
Time of Update: 2020-12-13
to measurement of the activity of each complex and analysis of their subunit compositionand provides information on the interactions between different complexes.
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Diabetologia: Complex interactions between an empty stomach's blood sugar, body mass index, age
Time of Update: 2020-12-13
The aim of this work was to check whether BMI and perinatal blood sugar (FBG) were associated with mortality and to identify high mortality FBG-BMI clusters by sex and age.
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JAMA Surg: B colonectomy is used to improve the quality of life of patients with relapse, complexity, or persistent painful diverticulitis
Time of Update: 2020-12-13
study concluded that B colonectomy was better than conservative treatment in improving the quality of life of patients with relapse, complexity or persistent painful diverticulitis, but 10% of patients had severe adverse events after surgery.
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Reconstitution of Active and Stoichiometric Multisubunit Lysine Acetyltransferase Complexes in Insect Cells
Time of Update: 2020-12-12
useful for assembling such multisubunit complexes. Related to this, we have adopted such an approach for reconstituting active tetrameric complexes ofmo nocytic leukemiaz inc (MOZ, finger protein, recently renamed MYST3 or KAT6A
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Rho GTPases: Deciphering the Evolutionary History of a Complex Protein Family
Time of Update: 2020-12-05
Second, we discuss the obstacles in reconstructing the history of gene duplications giving rise to the extant diversity of Rho paralogs in different species, and point to numerous limitations posed by the current phylogenetic methodology.
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Peptide Affinity Purification for the Isolation and Identification of GPCR-Associated Protein Complexes
Time of Update: 2020-12-05
Several GPCR-associated protein complexes (GAPCs), particularly those binding to the intracellular carboxyl-terminus (C-terminus), have been identified over the last 20 years.
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Purifi cation of Multiprotein Histone Acetyltransferase Complexes
Time of Update: 2020-12-04
These methods have been used to purify and characterize different human GCN5 HAT complexes that retain activity toward their physiological substrates in vitro.
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Sample Preparation for SFM Imaging of DNA, Proteins, and DNAProtein Complexes
Time of Update: 2020-12-03
We also describe special considerations for combined fluorescence and topographic imaging of molecular complexes.
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Use of Residual Dipolar Couplings in Structural Analysis of Protein-Ligand Complexes by Solution NMR Spectroscopy
Time of Update: 2020-12-01
Recent advances in NMR methodology such as transverse relaxation–optimized spectroscopy (TROSY) and residual dipolar couplings (RDCs) measured in liquid crystalline alignment medium, offer a viable alternative to traditional nuclear Overhauser enhancement (NOE)-based approaches for structure determination of large protein complexes.
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Analysis of 2-Aminoacridone-Derivatized Complex Oligosaccharides Using Micellar Electrokinetic
Time of Update: 2020-12-01
The clinical efficacy of a therapeutic glycoprotein may also be affected by the nature of its carbohydrate.
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Native Fractionation: Isolation of Native Membrane-Bound Protein Complexes from Porcine Rod Outer Segments Using Isopycnic Density Gradient Centrifugation
Time of Update: 2020-11-28
In this chapter we describe a protocol for native fractionation of membrane-bound protein complexes from isolated porcine rod outer segments (ROSs).
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Enhancing Antigen Cross-Presentation and T-Cell Priming by Complexing Protein Antigen to Recombinant Large Heat-Shock Protein
Time of Update: 2020-11-27
Therefore, reconstituted chaperone complexes of large HSPs and protein antigen may be exploited for augmentation of an antigen-specific immune response.
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Human Telomere POT1-TPP1 Complex and Its Role in Telomerase Activity Regulation
Time of Update: 2020-11-24
Through structural and biochemical studies, we have �demonstrated that human TPP1 is the missing human homolog of the β subunit of protozoan telomere end-binding-protein-complex (TEBPα-TEBPβ).
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Small-Angle Scattering and Neutron Contrast Variation for Studying Bio-Molecular Complexes
Time of Update: 2020-11-24
Further, exploitation of the different neutron-scattering properties of the stable isotopes of hydrogen ( 1 H and2 H) can be used to enrich the information available from the small-angle scattering data, especially for bio-molecular complexes.
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Epitope Mapping Antibody-Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy
Time of Update: 2020-11-24
and intramolecular interactions within the bound peptide ( 3 ). Alternatively, in the case of tight binding and slow exchange, isotope filtering methods can be applied ( 4 ). Tsang et al. ( 5 ) used this technique to elucidate
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Purification of Arabidopsis Argonaute Complexes and Associated Small RNAs
Time of Update: 2020-11-21
Argonaute (AGO) proteins recruit small RNAs to form effector complexes of RNA interference (RNAi), collectively termed RNA-induced silencing complexes (RISCs). Here, we describe detailed protocols for the purification of AGO complexes and their associated small RNAs, using Arabidopsis AGO1 as an example.
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Modified Bimolecular Fluorescence Complementation Assay to Study the Inhibition of Transcription Complex Formation by JAZ Proteins
Time of Update: 2020-11-20
Here we describe a modified BiFC experimental procedure to study the inhibition of the formation of the bHLH (GL3)–MYB (GL1) complex by JAZ proteins.
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The spray advantage of coating electrostectrectrecture powder friction spray gun on complex parts
Time of Update: 2020-11-20
With the continuous improvement of powder production level, more and more powder manufacturers produce spray powder suitable for high-pressure and friction gun applications.
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Extraction, Separation, and Analysis of Plant Phosphoinositides and Complex Glycolipids
Time of Update: 2020-11-19
Progress in the understanding of how cells perceive signals and how the perception of specific signals leads to cellular responses has been dramatic over the last decade.
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Isolation of Photosystem II Reaction Center Complexes From Plants
Time of Update: 2020-11-18
Methods to isolate and purify 6- and 5-chlorophyll (Chl) D1/D2/Cytb 559 photosystem (PS) II reaction center complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed.
