-
Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells
Time of Update: 2021-03-03
Here, we describe the use of safranine as a fluorescent indicator of Δ ψ in isolated mitochondria and digitonin-permeabilized cells.
-
Preparation of Single-Stranded Antisense cDNA Probes by Asymmetric PCR
Time of Update: 2021-03-03
The most commonly used approach is a technique called random priming ( 1 , 2 ), which uses random hexomers or nanomers to prime abundant quantities of purified template to extend into short complementary copies.
-
Methods to Isolate, Culture, and Study Osteoblasts
Time of Update: 2021-03-03
Primary cells are often desirable over clonal cell lines, because they are more likely to retain the presence and activity of certain enzymes and proteins that are often lost in clones.
-
A Practical Method of Chronic Ethanol Administration in Mice
Time of Update: 2021-03-03
In mice, it has proven to be necessary to administer ethanol daily for up to 32 wk or longer to observe all the immune abnormalities that occur in middle-aged alcoholic humans.
-
Analytical Methods for the Study of O-GlcNAc Glycoproteins and Glycopeptides
Time of Update: 2021-03-03
limited, in large part, because of the difficulty and the amount of protein required for mapping sites of glycosylation as a prerequisite to molecular manipulations such as site-directed mutagenesis. The primary method for mapping sites ofO
-
Methods for Measuring Class I Membrane-Bound Hyaluronan Synthase Activity
Time of Update: 2021-03-03
Three radioactive and nonradioactive assay methods are described that can give the amount of HA made with or without information about the distribution of product sizes.
-
Methods designed for the identification and characterization ofin vitro andin vivo chromatin assembly mutants inSaccharomyces cerevisiae
Time of Update: 2021-03-03
Additional modifications are described that allow for a rapid analysis and an in vivo characterization of yeast chromatin assembly mutants, as well as any other mutant of interest.
-
A fast, simple, and reliable high-yielding method for DNA extraction from different plant species
Time of Update: 2021-03-03
A rapid and versatile protocol for extracting high-quality DNA from different plant species is described.
The quality of the DNA was suitable for PCR amplification.
-
Methods for Analysis of Unusual Forms of O-Glycosylation
Time of Update: 2021-03-03
With these methods, we can determine both stoichiometry and the structure of the glycans on the expressed proteins.
-
Preparation of Direct, Enzyme-Labeled DNA Probes
Time of Update: 2021-03-02
The methodology was combined with enhanced chemiluminescence ( 2 ) (a light-producing HRP catalyzed reaction based on luminol) ( see Chapter 26 ) allowing the detection of specific hybrids on membranes ( 3 ).
-
A new method of more accurate epidemiological modeling
Time of Update: 2021-03-02
In the paper, scientists say standard epidemic models mistakenly believe that the rate at which infectious diseases spread depends on a simple product of the number of infected and susceptible populations.
-
Gene Probes
Time of Update: 2021-03-02
, with wideapphcatlons in molecular biology, medical, and environmental sciences. By defimtlon, a probe 1s aDNA molecule with a strongaffinity for a specific target, and the hybrid (probetarget combmatlon) can be revealed when an appropriate
-
Hybridization of Horseradish Peroxidase-Labeled Probes and Detection by Enhanced Chemiluminescence
Time of Update: 2021-03-02
This is shown by the diversity of applications in which this labeling and detection system has been used.
-
Methods for the study of ionic currents and Ca2+-signals in isolated colonic crypts
Time of Update: 2021-03-02
often are caused by an inadequate activation of intestinal anion secretion, are one of the major lethal diseases of children or young animals. The aim of this paper is to describe a method for the isolation of intact colonic crypts, e.g
-
Sales soared and companies such as Johnson and Johnson used this method
Time of Update: 2021-03-01
May 14, according to the National Health and Health Commission, on the 13th of this month, 0-24 hours, three new confirmed cases, all local cases (2 cases in Liaoning, 1 case in Jilin); We can see that China
-
Enzyme-Labeled DNA Probes
Time of Update: 2021-03-01
The presence of certain types of human papillomavirus in cervical smears can be used as an indicationof neoplasia ( 2 ).
-
A method for rapidly screening functionality of actin mutants and tagged actins
Time of Update: 2021-02-28
We therefore have developed a method to rapidly screen the folding capacity and functionality of actin variants, by combiningin vitro expression of labelled actin with analysis on native gels, band shift assays or copolymerization tests.
-
Precise large deletions by the PCR-based overlap extension method
Time of Update: 2021-02-28
The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the PCR -based method of gene splicing by overlap extension ( 1 ). This method is technically simple and less
-
Detection of DNA Sequences Using Biotinylated Probes
Time of Update: 2021-02-28
Figure 1 shows the structure of biotin-11-dUTP, a commonly used substrate for biotinylation by the method of nick translation (2 ).
-
A novel method for preparing single-stranded DNA for pyrosequencing
Time of Update: 2021-02-28
In this article, an enzymatic liquid-phase method for the generation of high-quality, single-stranded DNA, and its novel use for Pyrosequencing™ are described.
The method has also been fully automated.
