[Gut] Imaging mass spectrometry flow cytometry helps the topological analysis of hepatocellular
Time of Update: 2021-08-27
stages . Type I: Hepatocyte markers, such as pan-keratin and E-cadherin are strongly expressed. The main liver structures, including sinusoids and hepatic cord, are preserved intact
Full-spectrum flow cytometry Cytek creates a new ecology of multi-color cell analysis and sorting
Time of Update: 2021-08-03
Cytek cFluor TM immunoprofiling kit Strong market outlook Cytek Biosciences is headquartered in Silicon Valley, USA, and is committed to the development of innovative flow cytometry technology to provide solutions for life science research and clinical diagnosis and treatment .
Assay of Intracellular Hydrogen Peroxide Generation in Activated Cytometry Individual Neutrophils by Flow
Time of Update: 2021-03-01
It provides a sensitive assay to measure intracellular ROS in individual neutrophils ( 2 , 3 ).
Analysis of Receptor Tyrosine Kinase Internalization Using Flow Cytometry
Time of Update: 2021-02-27
To understand how cMet signaling for normal cell growth is controlled by endocytosis and how these mechanisms are dysregulated in metastatic cancers, we developed flow cytometry-based assays to examine cMet internalization.
Examination of Bacillus anthracis Spores by Multiparameter Flow Cytometry
Time of Update: 2021-02-24
anthracis strains are fully virulent and the ability to determine the potential virulence of the endospore is also important.
Analysis of the DNA of Parasitic Protozoa by Flow Cytometry
Time of Update: 2021-02-20
Parasitic protozoa, generally, present as highly disperse popula tions with complex life cycles.
Isolation of Carotenoid Hyperproducing Mutants of Xanthophyllomyces dendrorhous (Phaffia rhodozyma) by Flow Cytometry and Cell Sorting
Time of Update: 2021-02-19
Approaches for improving astaxanthin yields inXanthophyllomyces dendrorhous include optimization of fermentation conditions and generation of hyperproducing mutants through random mutagenesis using chemical or physical means.
In Situ Analysis of Chromatin Proteins During Development and Cell Differentiation Using Flow Cytometry
Time of Update: 2021-02-19
For instance, duringXenopus laevis early development, the midblastula transition (MBT) is characterized as a period after which the somatic replication-dependent type of H1 starts to accumulate and the embryonic H1 (B4) and HMG1 decrease dramatically ( 7 ).
Viability of Amoebae, Fungal Conidia, and Yeasts: Rapid Assessment by Flow Cytometry
Time of Update: 2021-02-17
Rapid detection and viability measurements of microorganisms in homogenous and heterogenous microbial populations have been greatly enhanced by recent advances in the use of fluorescent stains in flow cytometry (FCM) ( 1 – 5 ).
Enumeration of Bacteriophages Using Flow Cytometry
Time of Update: 2021-02-17
The present paper describes an optimized protocol for the enumeration of bacteriophages using a standard benchtop flow cytometer.
Flow Cytometry in Environmental Microbiology: A Rapid Approach for the Isolation of Single Cells for Advanced Molecular Biology Analysis
Time of Update: 2021-02-16
We describe here the combination of fluorescent in situ hybridization and cell sorting for the detection and isolation of Candidate Division TM7 bacteria from an enriched soil sample.
Detection of Infectious Rotaviruses by Flow Cytometry
Time of Update: 2021-02-13
However, the sensitivity of these procedures is not high enough to detect the low number of viral particles sometimes present in the environment ( 3 ).
Immunological Techniques: ELISA, Flow Cytometry, and Immunohistochemistry
Time of Update: 2021-02-01
A number of techniques are used extensively by researchers investigating humoral and cellular immune responses to oral organisms both in local oral tissues and fluids and systemically in peripheral blood.
Analysis of Heat-Shock Protein Localisation Using Flow Cytometry
Time of Update: 2021-01-03
As a result, flow cytometry is becoming the leading technique used in this area of research.
Analysis of Subcellular Components by Fluorescent-Lectin Binding and Flow Cytometry
Time of Update: 2021-01-02
Because of their extensive availability and the wide spectrum of carbohydrates that may be specifically bound, lectins havebecome essential reagents for detection and quantitation of glycoconjugates in solution and in cell surfaces, identificationand separation of cells, and functional studies based on membrane properties ( 1 , 2 )
Assessment of Cellular Uptake and Cytotoxicity of Carbon Nanotubes Using Flow Cytometry
Time of Update: 2020-12-29
The assay described here allows for rapid screening of CNT cellular uptake in vitro and assessing the acute cytotoxicity simultaneously.
Using Fluorescence-Activated Flow Cytometry to Determine Reactive Oxygen Species Formation and Membrane Lipid Peroxidation in Viable Boar Spermatozoa
Time of Update: 2020-12-17
Fluorescence-activated flow cytometry analyses were developed for determination of reactive oxygen species (ROS) formation and membrane lipid peroxidation in live spermatozoa loaded with, respectively, hydroethidine (HE) or the lipophilic probe 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid, C 11 BODIPY 581/591 (BODIPY).
Micro-scale flow cytometry-based and biochemical analysis of lipid signaling in primary B cell subpopulations
Time of Update: 2020-12-14
Additionally, utilizing flow cytometric techniques, we determined levels of phosphatidylinositol bisphosphate and calcium mobilization in T1 and T2 cells, as well as mature follicular and marginal zone B cells using less than 1�10 6 primary B cells.
Detection of Reactive Oxygen Species by Flow Cytometry
Time of Update: 2020-11-30
Oxidant stress is involved in the pathogenesis of many cardiovascular diseases and is closely related to vascular endothelial dysfunction ( 1 ).