c DNA clones of genes expressed in small amounts of material can be hard to obtain because the construction of conventionalcDNA libraries requires microgram amounts of poly (A) + RNA ( 1
Preparation of Single-Stranded Antisense cDNA Probes by Asymmetric PCR
Time of Update: 2021-03-03
The most commonly used approach is a technique called random priming ( 1 , 2 ), which uses random hexomers or nanomers to prime abundant quantities of purified template to extend into short complementary copies.
Enzyme Stabilization via Bio-templated Silicification Reactions
Time of Update: 2021-03-03
A silica sol-gel process based upon biological mechanisms of inorganic material formation (biomineralization) supports protein immobilization reactions within minutes.
Aggregate reactions
Time of Update: 2021-03-01
For example, aggregation reaction that occurs in two or more monomers is called a co-polymerization reaction.
Construction of Synthetic Genes by Polymerase Chain Reaction
Time of Update: 2021-02-28
The method described in this chapter utilizes the thermostableTaq polymerase and allows for the generation of synthetic genes in as little as 1 d.
JACI: Study reveals molecular mechanisms for severe allergic reactions
Time of Update: 2021-02-28
lead to death. reactions can be caused by a variety of causes, such as allergic reactions to food, drugs, or insect venom. molecular mechanisms that cause such reactions to be severe are still unknown. study, led by researchers
Quantitative TaqMan Real-Time PCR: Diagnostic and Scientific Applications
Time of Update: 2021-02-28
As soon as resources are exhausted, however, the so-called plateau phase of PCR reaction is reached, making quantification very unreliable.
Precise large deletions by the PCR-based overlap extension method
Time of Update: 2021-02-28
The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the PCR -based method of gene splicing by overlap extension ( 1 ). This method is technically simple and less
PCR-Based Construction of Long Hammerhead Ribozymes
Time of Update: 2021-02-27
More recently, there have been numerous reports that the incorporation of either hairpin or hammerhead ribozyme motifs (catalytic antisense) into such RNAs increases their effectiveness ( 1 – 3 ).
PCR in the genetic diagnosis of viral infection
Time of Update: 2021-02-27
total RNA extraction using reverse transcriptase synthesis c DNA PCR reaction: reactor tissue: reaction buffer template (synthetic cDNA
Multiplex Polymerase Chain Reaction
Time of Update: 2021-02-27
When these assays must be performed repetitively on multiple samples, the multiplex PCR, which allows for simultaneous amplification of 2–50 different targets ( 13 – 15 ), is potentially very useful from a perspective of time, and money.
BMJ: Milk is the most common cause of fatal allergic reactions in children in the UK
Time of Update: 2021-02-26
, there was an increase in hospital admissions for food-related allergic reactions in the UK, but a decrease in the number of deaths. in school-age children, milk allergies are the single most common cause of fatal allergic reactions.
In Situ PCR Amplification of Intracellular mRNA
Time of Update: 2021-02-26
However, conventional RT-PCR cannot be used either to quantitate the frequency of cells expressing a particular mRNA or determine the cellular origin of the amplified signal.
An effective method of completely removing contaminating genomic DNA from an RNA sample to be used for PCR
Time of Update: 2021-02-25
This method produces an RNA sample that is negative for genomic DNA byPCR .
